Rapid analysis of components in Rhizoma Anemarrhenae by HPLC-DAD-MS and HPLC-DAD-TOFMS

A global quality control method based on high performance liquid chromatography (HPLC) coupled with diode array detection (DAD), single quadrupole mass spectrometry (MS) and time-of-flight mass spectrometry (TOFMS) was developed for simultaneous determination of seven major components (mangiferin, neomangiferin, timosaponin E1, timosaponin E, timosaponin BⅡ, timosaponin BⅢ, and timosaponin AⅢ) and identification of most components in extracts of Rhizoma Anemarrhenae (RA). HPLC analysis was performed on an Agilent SB-C18 column (4.6 mm×150 mm, 5 μm) by gradient elution using acetonitrile and water-acetic acid(100∶0.05, v/v) as the mobile phase. Seven major components in RA were successfully separated. This quantitative method was fully validated in respect of the following performance criteria: linearity, precision, repeatability, stability, accuracy, limits of detection (LOD) and quantification (LOQ). A formula database of known compounds in RA was established, against which, most of the reported components in this herbal extract were identified effectively based on the extract masses acquired by TOFMS. This qualitative and quantitative method was successfully used to analyze the components in 10 batches of RA samples collected from different regions in China. This global quality control method, which consisted of HPLC-DAD-MS assay of seven major components and unambiguous identification of nineteen components, is suitable for routine quantification and comprehensive quality control of RA.     

RP-HPLC method for the simultaneous determination of daidzein,genistein and formonetin in Solanum Lyratum Thunb

A rapid method for the simultaneous determination of daidzein, genistein and formonetin in solanum Lyratum Thunb by high performance liquid chromatography (HPLC) was developed. Separation was achieved on a Diamonsil C18 column (250 mm×4.6 mm, 5 μm) with isocratic elution, using a mobile phase of methanol-tetrahydrofuran-water (44∶3∶53, v/v). The wavelength was set at 260 nm and column was maintained at 35 ℃. The linear ranges of daidzein, genistein and formonetin were 1.0-40.0, 0.1-4.0 and 0.1-4.0 μg/mL, respectively. The average recoveries were between 98.4% and 101.3%. This method could be used for the quality control of Solanum lyratum Thunb due to its simplification, reliability, rapidity and excellent precision.     

大气环境中微量甲醛的色谱测定法

叙述了用色谱法测定大气环境中微量甲醛的各种方法，其中包括气相色谱法，液相色谱法和高效液相色谱法，离子色谱法，薄层色谱法和气相色谱法－质谱法，就有关的内容做了介绍。     

兔组织内吗啡的免疫组织化学研究

为建立一个从病理组织诊断吗啡中毒的方法，研究吗啡在体内的分布，应用盐酸吗啡针剂对家兔进行慢性染毒，采用免疫荧光技术，原位观察吗啡在染毒家兔肝、脾、肾及脑组织中的分布及各脏器中吗啡含量的差异，同时采用 HPLC检测同一取材组织中的吗啡含量。通过对比分析这两项指标，认为吗啡免疫荧光组织化学技术可以作为一个简便、快速、较特异地鉴定吗啡类中毒的方法。     

柴油机燃用DME的非常规排放物试验研究

用气相色谱仪测量研究了ZS195柴油机燃用DME排气中的非常规排放物甲醛和甲酸甲酯。研究表明,甲醛是非常规排放物中的主要成分;在空载工况下,甲醛和甲酸甲酯的排放量随转速上升而略有上升;在高负荷工况下,二者的排放量大幅降低;此外,适当减小供油提前角,降低喷油压力和缩小喷孔直径都有利于减少甲醛和甲酸甲酯的排放量。     

Quantification of six bioactive compounds in Zhenqi Fuzheng preparation by high-performance liquid chromatography couple

A simple and accurate high-performance liquid chromatography（HPLC）coupled with diode array detector（DAD）and evaporative light scattering detector（ELSD）was established for the determination of six bioactive compounds in Zhenqi Fuzheng preparation（ZFP）.The     

Applications of HPLC/MS in the analysis of traditional Chinese medicines

In China, traditional Chinese medicines （TCMs） have been used in clinical applications for thousands of years. The successful hyphenation of high-performance liquid chromatography （HPLC） and mass spectrometry （MS） has been applied widely in TCMs and biolo     

不同品牌DNPH管对车内醛酮类化合物测试影响的研究

研究了二硝基苯肼（DNPH）管的背景值，重点探讨了不同品牌DNPH管对甲醛、乙醛和丙醛回收率的影响以及同一品牌DNPH管对不同醛类物质回收率的影响，与采样条件对醛类物质收回率的影响，发现不同品牌对甲醛和乙醛的回收率影响较小，但对丙烯醛的回收率影响较大，同样，不同采样条件对甲醛和乙醛的回收率影响较小，对丙烯醛的回收率影响较大。