表达性艺术——藏族舞蹈锅庄对心理咨询与治疗的启示

表达性心理治疗是一种新兴的心理治疗的技术,它通过游戏、活动、音乐、绘画、舞蹈、戏剧等艺术媒介对来访者进行心理咨询与治疗.锅庄舞蹈是表达性艺术,与表达性心理治疗具有异曲同工之妙,对心理放松与治疗具有一定的辅助作用,适合于团体心理治疗使用,研究藏族舞蹈锅庄的心理治疗作用,有助于建立一种本土化的团体心理辅导的模式.     

对剽窃行为认定的路径

剽窃行为在侵害著作权人利益的同时,也破坏了广大受众的信赖利益,极不利于学术发展和文化传播。我国现行法律并没有对剽窃行为的要件进行规定,只得依靠司法实践中的各种方法对剽窃进行认定。为促进裁判结果统一,提高认定效率,本研究综合已存在的各种认定方法,提出了确定原告权利基础、考察剽窃可能性、排除部分作品内容、比较剩余相似部分等先后四个环节的认定路径。     

关于“航线”一词的英语表达

2002年第6期<集装箱化>杂志上刊登了署名为王家斌的<浅析船行"路线"的英语表达>一文,其中提到,"line一词是船公司使用频率最高的一词.人们口头常说的‘美东线’、‘澳新线’等均使用line或tradeline……".看过后,笔者也有兴趣根据多年从事航运工作的实践,尤其是依据个人近年来与主要国际知名航运企业交往和对国外权威航运出版物的研究,对"航线"一词的英语表达,谈一点自己的观点.     

斯利姆·埃卢：挨骂的世界新首富

卡洛斯·斯利姆·埃卢——一个从墨西哥走出的符号式人物．似乎并没有像盖茨那样成为众人传唱的英雄，相反由于其特殊的财富成长背景和非常另类的观点表达而招来了不小的非议。因此．等待斯利姆·埃卢的也许是前行路上更加严峻的考验与挑战。[第一段]     

Eukaryotic expression and biological activity analysis of neuroprotective peptide [Gly14]-Humanin

Objective To investigate the expression of neuroprotective peptide [Gly14]-Humanin (HNG) in eukaryotic cells by gene engineering technique and analyze its biological activity. Methods By means of asymmetrical primer/template, double stranded cDNA of HNG with FLAG in its C-terminal was obtained, which was cloned into the plasmid pcDNA3.1(-), and the resultant recombinant vector pcDNA3.1(-)/HNG-FLAG was transfected into PC12 cells. At the same time, the recombinant vector pcDNA3.1(-)/EGFP was transfected to control the efficiency of transfection. The expression of HNG in the cells was determined by immunocytochemistry. In order to analyze the biological activity of the expressed HNG, 25μM Aβ25-35 peptide was added to the culture medium of the transfected cells for 24h, then cell morphology, MTT assay and Hoechst 33258 staining were observed. Results The eukaryotic expression vector of pcDNA3.1(-)/HNG-FLAG was identified by enzyme digestion and sequencing. HNG was highly expressed in PC12 cells. After exposure of PC12 cells to 25μM Aβ25-35 for 24h, cell viability decreased to (65.8±5.3)%, and the dystrophic changes of neuritis and nuclei condensation were obvious. When cells were pre-transfected with pcDNA3.1(-)/HNG-FLAG, Aβ25-35-induced cell death and morphological changes of cells and nuclei were suppressed. In contrast, pre-transfected with empty vector did not protect cells from Aβ25-35-induced toxicity. Conclusion The eukaryotic expression vector for FLAG-tagged HNG was successfully constructed and expressed in PC12 cells. Expressed HNG has biological activity.     

Construction of lentivirus vectors carrying alphastatin gene and its secretion expression in human umbilical vein endothelia cells

Objective To construct lentivirus vectors carrying alphastatin gene, test its secretion expression in human umbilical vein endothelia cells (HUVECs) and observe its effects on growth, migration and tube formation of HUVECs. Methods We constructed recombinant lentivirus vectors of NT4-alphastatin fusion gene containing neurotrophin-4 signal peptide, pro-region sequences and alphastatin, then transfected the recombinant lentivirus vectors into HUVECs to obtain secretory protein alphastatin and test its anti-angiogenic activities in vitro. Results Our data showed that recombinant self-inactivating lentivirus vectors of NT4-alphastatin were successfully constructed, and stable NT4-alphastatin transduced HUVECs were capable of sustainably secreting alphastatin which significantly suppressed HUVECs migration and differentiation but not VEGF-induced proliferation. Conclusion This report represents the first time on the use of lentivirus-based vectors to deliver alphastatin, the endogenous angiogenesis inhibitor, and reveals the potential utility of anti-angiogenic gene therapy with lentivirus vectors for treating cancer.     

HBV preS1基因酵母表达载体的构建及表达

目的 构建HBV preS1基因的酵母表达载体,探讨HBV preS1蛋白的功能.方法 以HBV ayw亚型全长质粒PCP10为模板,聚合酶链反应(polymerase chain reaction, PCR)扩增HBV preS1基因,克隆到pGEM-T载体中命名为T-preS1,以NcoⅠ和MluⅠ双酶切T-preS1后回收与酵母表达载体pSos连接,对重组质粒进行序列测定后命名为pSos-preS1,经醋酸锂法将其转化酵母菌cdc25(a),提取酵母蛋白质进行Western免疫印迹分析.结果 成功构建了HBV preS1基因的酵母表达载体,Western免疫印迹分析显示HBV preS1基因在酵母细胞中正确表达.结论 pSos-preS1的构建为通过SOS招募系统(sos-recruitment system, SRS)筛选与HBV preS1蛋白相互作用的蛋白和进一步探讨HBV preS1蛋白在HBV致病中的作用奠定了基础.     

Glutamate enhances the expression of vascular endothelial growth factor in cultured SD rat astrocytes

Objective To study the effect of glutamate on the expression of vascular endothelial growth factor (VEGF) mRNA and protein in cultured rat astrocytes. Methods Cultured rat astrocytes were randomly divided into 6 groups: control group (C), glutamate group (G), QA group (Q), DCG-IV group (D), L-AP4 group (L) and glutanmte-FMCPG gronp (G+M). Cells were cultured under nomoxic condition (95% air, 5% CO2). RT-PCR and ELISA methods were used to detect the expression of VEGF mRNA and protein in cultured astrocytes, respectively. G+ M group was preincubated with lmM MCPG for 30 min prior to the stimulation with glutamate. There were 7 time points at 0,4,8,12,16,24 and 48 h in each group except G+M group. Results The expression of VEGF mRNA and protein did not differ significantly among D group, L group and C group. Different from that in C group, the expression of VEGF mRNA and protein could be enhanced both in a dose-dependent and time-dependent manner in G group and Q group. Meanwhile, the enhanced expression of VEGF mRNA and protein in G group was completely suppressed by MCPG after 24 h. Conclusion Glutamate can increase the expression of VEGF mRNA and protein in cultured astrocytes, which may be due to the activation of group I metabotropic glutamate receptors in astrocytes.