Analysis of chiral non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac binding with HSA

The protein binding of non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac with human serum albumin （HSA） was investigated using indirect chiral high performance liquid chromatography （HPLC） and ultrafiltration techniques. S-（-）-1-（1-naphthyl）- ethylamine （S-NEA） was utilized as chiral derivatization reagent and pre-column derivatization RP-HPLC method was established for the separation and assay of the three pairs of enantiomer. The method had good linear relationship over the investigated concentration range without interference. The average extraction efficiency was higher than 85% in different systems, and the intra-day and inter-day precisions were less than 15%. In serum albumin, the protein binding of etodolac enantiomers showed significant stereoselectivity that the affinity of S-enantiomer was stronger than R-enantiomer, and the stereoselectivity ratio reached 6.06; Flurbiprofen had only weak stereoselectivity in HSA, and ketoprofen had no stereoselectivity at all. Scatchard curves showed that all the three chiral drugs had two types of binding sites in HSA.     

送走2010:曼公司用高效驾驶活动画上圆满句号

2010年就像一艘航行在波涛汹涌的海面上的巨轮,在起伏中前进,坚定驶向彼岸。中国虽然又经历了一个多灾之年,但快速的经济增长带动全球从2009年的低迷中走向复苏。曼公司2010年在华形势也好于全球,特意在接近年底的11月份举办了高效驾驶活动,体现了曼公司始终以用户关怀为核心理念,并为2010年画上圆满句号。     

更节能更高效——希腊船东表示，集装箱船将从新的节能设计中获得最大利益。

“集装箱船舶将受益于节能高效的船舶设计。集装箱船无论对干散货船或者油轮来说都具有压倒性优势，在一定范围内能够产生巨大的经济效益。”希腊船东Technomar现任掌门人GeorgeYouroukos表示，新的节能设计可以为船舶节省燃油，每艘干散货船或者油轮每天能够节省大约4-6吨燃油，集装箱船则可能节省高达30吨的燃油，这是因为集装箱船引擎最为强大。     

我国船机企业技术升级亮点频现

回望2013这一年，我国船用柴油机产业亮点频现，骨干企业应对危机有方，纷纷瞄准绿色环保、节能高效等需求，在各自的细分市场加大研发创新力度，推出优化更新或研发成功的新机型，有的甚至是世界首制机型。     

山船重工多项修船工程获船东好评

<正>目前,山船重工在修船舶16艘,近期还将有12艘船舶进厂修理。据统计,仅7月份,通过修船厂和各生产单位的共同努力,公司修船完工船舶22艘。施工人员在保障节点进度的同时,严格控制工程质量,优质高效地完成了"莱芜钢铁"和"亚美6号"等船舶的机电、钢结构、涂装等工程,并获得船东好评。俄罗斯渔船受备件多为异型、不易加工和采购等因素的影响,工期一度出现紧张局面。为保证施工进     

高效管理和诚信服务：客户的选择——广东中远船务全力推进企业产品转型升级系列可报造之三

坚持以产品实现为目标，不断完善安全、质量、成本、物资、服务等专业管理体系，广东中远船务以高效的生产服务体系擦亮“COSCOSHIPYARD”华南品牌，以过硬的制造实力赢得了市场认同。     

Comparative pharmacokinetics of chlorogenic acid after oral administration in rats

The present study was aimed at the comparison of the pharmacokinetics of pure chlorogenic acid and extract of Solanum lyratum Thunb. The animals were allocated to two groups, and were administered chlo...     

曼欲做高效物流解决方案领跑者

如何应对高端商用车市场的激烈竞争,并在竞争中建立自身优势,曼商用车(中国)总裁林建兴先生在接受专访时表示,高端商用车竞争不是仅仅体现在产品、价格层面,更多的是综     

Published the papers of HPLC-MS analysis—Traditional Chinese medicine

Medicinal materials Ma Chenchen, Li Bailin, Ou Jie, Wang Jing, Zhao Junhong. Detection of N-acyl-homoserine lactones class signal molecules of quorum sensing secreted by bacteria using high performance liquid chromatography-mass spectrometry/ mass spect     

多敏灵片中咖啡因和盐酸多塞平含量的测定

目的建立高效液相色谱法,测定多敏灵片中咖啡因、盐酸多塞平的含量。方法采用高效液相色谱法,以Kromasil C18(250 mm×4.6 mm,5μm)为分析柱,流动相为甲醇-水-三乙胺(75∶25∶1),流速1 mL/min,检测波长295 nm,柱温为室温。结果咖啡因、盐酸多塞平与其他组分分离良好,且不受其他成分的干扰;咖啡因回归方程为:Y=196 604X+218 976(r=0.999 8,n=5),咖啡因在0.08~0.24μg的范围内线性关系良好;盐酸多塞平回归方程为:Y=60 158X+58 230(r=0.999 9,n=5),盐酸多塞平在0.04~0.12μg的范围内线性关系良好。方法的平均回收率分别为99.06%、96.67%,精密度(RSD)值分别为1.04%、1.13%。结论本方法操作简便、快速、准确、灵敏,适用于多敏灵片的含量测定。