SS3B固定重联机车的研制

系统介绍了由2节SS3B机车经过技术改造成12轴重载货运SS3B固定重联机车；重点介绍了机车的主要结构、性能参数和总体布置，同时简述了机车的主要系统和特点。试验表明，机车的改造是成功的。     

南京长江第三大桥钢塔柱设计与加工

南京长江三桥钢混结合区置于索塔下横梁顶面。为避免涡激振动和驰振,通过10种切角方案和3种切角形式断面不同攻角情况下风洞试验对塔柱进行选型,并在塔端设置一组制振设备,以控制索塔架设过程中的振动。节段连接采用高强度螺栓。使用ANSYS程序进行结构有限元分析,对最不利的施工状态进行检算。在工厂制造板块和锚箱,在工地组焊钢塔柱节段,在桥位进行钢塔柱节段安装。通过焊接评定试验,确定焊接工艺和焊接顺序,采用大型数显端面加工落地铣镗床和精密测量设备,确保加工精度。     

3ds网格模型技术在航海上的应用

在读取3ds文件中的网格模型数据时,通常认为只读取编辑块内部的数据即可再现模型,其实不然。本文介绍如何利用关键帧块中的数据真实再现三维网格模型,并应用到航海上。     

道路整体三维模型构建方法的研究

建立道路与地面的整体三维模型是实现道路三维可视化的关键。系统分析了现有的道路三维模型构建方法,针对其存在的不足,提出了一种将道路设计面模型与地形表面模型融为一体的整体模型构建方法,该方法基于在约束Delaunay三角网内插入点和约束边的理论,巧妙地解决了剔除落在道路设计面区域内的地形点的问题,实现了道路设计面与地形表面的整体建网。应用道路整体模型可以实现道路景观的三维漫游,评价三维立体线形以及与周围地形的配合情况。     

08-32型正线捣固车和08-475型道岔捣固车拨道系统几何原理分析

详细分析了08－32型正线捣固车和08－475型道岔捣固车三、四点法检测拨道的几何原理和实现方法，阐述了08－475型道岔捣固车拨道测量弦随动系统的补偿几何原理，并推导出了各参数之间的几何关系。为拨道系统的电气控制及电气调试提供了理论依据。     

地铁车站单双层过河段沉降计算分析

探讨地铁车站单双层地铁站过河段沉降差,采用土的Coulomb-Mohr破坏与屈服准则建立模拟施工过程(包括土体自重变形、开挖、回填等)的三维有限元力学模型,得到施工过程引起的周围岩土应力变化、沉降变形以及地铁单双层过河段的沉降差值.单双层车站最大沉降控制在3 cm以内,最大沉降差控制在1 cm以内,能够满足设计要求.对二维计算结果进行校核,车站单双层的底板、顶板、侧板与中柱在施工过程中各个工况均满足设计要求.     

Urban form,travel time,and cost relationships with tour complexity and mode choice

The primary purpose of this study was to investigate how relative associations between travel time, costs, and land use patterns where people live and work impact modal choice and trip chaining patterns in the Central Puget Sound (Seattle) region. By using a tour-based modeling framework and highly detailed land use and travel data, this study attempts to add detail on the specific land use changes necessary to address different types of travel, and to develop a comparative framework by which the relative impact of travel time and urban form changes can be assessed. A discrete choice modeling framework adjusted for demographic factors and assessed the relative effect of travel time, costs, and urban form on mode choice and trip chaining characteristics for the three tour types. The tour based modeling approach increased the ability to understand the relative contribution of urban form, time, and costs in explaining mode choice and tour complexity for home and work related travel. Urban form at residential and employment locations, and travel time and cost were significant predictors of travel choice. Travel time was the strongest predictor of mode choice while urban form the strongest predictor of the number of stops within a tour. Results show that reductions in highway travel time are associated with less transit use and walking. Land use patterns where respondents work predicted mode choice for mid day and journey to work travel.

Modeling children’s school travel mode and parental escort decisions

Understanding of the activity-travel patterns of children is becoming increasingly important to various policy makers. Further, there is also a growing recognition that intra-household interactions need to be explicitly accommodated in travel models for realistic forecasts and policy evaluation. In the light of these issues, this paper contributes towards an overall understanding of the school-travel behavior of children and the related interdependencies among the travel patterns of parents and children. An econometric model is formulated to simultaneously determine the choice of mode and the escorting person for children’s travel to and from school. The 2000 San Francisco Bay Area Travel Survey (BATS) data are used in the model estimation process. Empirical results indicate that the characteristics of child like age, gender, and ethnicity, and employment and work flexibility characteristics of the parents have strong impacts on the mode choice decisions. In addition, the impacts of some of these attributes on the choice of mode to school are different from the corresponding impacts on the choice of mode from school. The distance between home and school is found to strongly and negatively impact the choice of walking to and from school, with the impact being stronger for walking to school. Several land-use and built-environment variables were explored, but were found not to be statistically significant predictors.

三维植被网护坡技术在哈同公路的应用

介绍了边坡生态防护技术和三维植被网护坡技术在哈同公路的应用。     

多房棘球绦虫重组Bb-Em14-3-3疫苗的构建及其表达效率

目的构建多房棘球绦虫(Em)重组双歧杆菌(Bb)-Em14-3-3疫苗,并研究Em14-3-3分子在大肠埃希菌BL21(DE3)中的表达效率。方法通过PCR扩增Em14-3-3抗原编码基因;将该基因定向克隆于含有谷胱甘肽-S-转移酶(GST)基因的大肠埃希菌-双歧杆菌穿梭表达载体pGEX-1λT,构建重组质粒pGEX-Em14-3-3;用电穿孔法将该质粒导入Bb,构建多房棘球绦虫重组Bb-Em14-3-3疫苗。经PCR和酶切鉴定后以IPTG诱导表达Em14-3-3/GST融合蛋白;SDS-PAGE及Western blot对表达产物进行鉴定。结果PCR成功扩增出530 bp的Em14-3-3基因;双酶切证实Em14-3-3基因插入pGEX-1λT中;PCR证实重组Bb-Em14-3-3疫苗构建成功;SDS-PAGE及Western blot分析显示重组质粒转化宿主菌在IPTG诱导下高效表达了Em14-3-3/GST融合蛋白,表达效率为23%。结论成功构建了多房棘球绦虫重组Bb-Em14-3-3疫苗,重组质粒pGEX-Em14-3-3在大肠杆菌中获得了高效表达,Western blot结果提示表达出的Em14-3-3重组蛋白具有特异抗原性。