浙江省B2C电子商务企业物流模式探讨

B2C电子商务企业能取得多大的成功很大程度上依赖于实际物流的操作,B2C电子商务企业必须根据自身情况,正确选择和采用企业自营物流模式或是第三方物流模式。本文根据实地调研的数据,就浙江省B2C电子商务企业的物流模式存在的问题进行分析探讨,寻求成本低廉、需求响应快速、安全可靠且适宜的物流模式。     

基于ASP的在线考试系统的设计及应用

一个完整的在线考试系统应该包括考试用户注册系统、在线考试系统、分级账户及试卷管理系统、用户成绩查询系统、帮助系统等。本文提出了一种基于Asp的在线考试系统解决方案,具有移植性好、易于实现等优点。     

浅析呼和浩特西站计算机联锁改造

本文介绍了呼和浩特西站(DS6-K5B 计算机联锁系统)与呼和浩特站增加三线工程改造施工的相关过程和经验,重点阐述了大型站场含控制台改造需要注意的问题。     

Methylation status of the interferon-gamma gene promoter in chronic hepatitis B

Objective To evaluate the methylation status at CpG site -55 in the interferon-gamma (IFN-7) gene promoter and its effect on IFN-7 expression in chronic hepatitis B. Method The authors recruited 30 patients with UBeAg-positive chronic hepatitis B (CHB), 30 HBeAg-negative CHB patients, and 30 healthy blood donors. Pyrosequeneing was used to determine the methylation status at CpG site -55 in the IFN-γ gene promoter following bisulfite treatment of DNA in peripheral blood mononuclear cells (PBMCs). The expression of IFN-γ was analyzed by real-time RT-PCR and ELISA. HBV DNA in PBMCs was detected by nested PCR. Results The methylation level at CpG site -55 in the IFN-γ gene promoter was significantly increased, resulting in subsequent down-regulation of the expression of this cytoldne in CHB. The methylation level at CpG site -55 was significantly higher in HBeAg-positive patients than in HBeAg-negative ones (P<0.01) and was also significantly higher in PBMCs from HBV DNA-positive patients than from HBV DNA-negative ones (P<0.01) ; the methylation level at CpG site -55 was positively correlated with the amount of HBV DNA in serum (P<0.01). Oonclusion IFN-γ gene expression appears to be regulated by methylation of the IFN-γ gene promoter in CHB; the methylation level at CpG site -55 is associated with HBV infection.     

Cathepsin B antisense oligodeoxynucleotide suppresses invasive potential of MG-63 cells

Objective To study the biological effects of cathepsin B phosporotbioated antisense oligodeoxynucleotide on human osteosarcoma cell line MG-63 after transfection. Methods A 18-mer phosphorothioate antisense oligodeoxynucleotide (ASODN) targeted against the cathepsin B mRNA was transfected into the human osteosarcoma cell line MG-63 by lipofectamine 2000. The sense and nonsense oligodeoxynucleotides to cathepsin B and blank vector were used as controls. The expression of cathepsin B mRNA was examined by RT-PCR and the expression of cathepsin B was examined by Western blot. The invasive capability of MG-63 cells was evaluated by the boydern chamber assay. Results The expression of cathcpsin B was obviously inhibited in antlsense oligodeoxynucleotide treated cells compared with the control cells. The number of invading MG-63 cells was significantly lower in the ASODN-treated groups than that in the control groups. Conclusion The cathepsin B ASODN significantly inhibits the expression of cathepsin B and invasive ability of MG-63 cell in osteosarcoma.     

间接法和捕捉法检测特异性IgM的敏感性与特异性的比较

目的比较捕捉法和间接法检测IgM时的特异性和敏感度差异,并探讨其产生差异的原因。方法分别用间接ELISA法和捕捉ELISA法检测活动性乙型肝炎和肾综合征出血热(HFRS)患者标本的IgM水平,并比较两者之间检出率、检测阈值、特异性的差异。结果检测稀释在PBS中的鼠抗IgM特异性抗体,两种方法的特异性和敏感度类似;检测活动性乙型肝炎和HFRS患者标本,间接法检测IgM的敏感度明显低于捕捉法;检测经二巯基乙醇处理后乙肝组和HFRS组标本,两种检测均为阴性,表明两种方法都具有针对IgM抗体的型特异性;交叉实验结果证明两种检测IgM的试剂均具有针对抗原的特异性。结论急性感染疾病的早期诊断、病毒的潜伏期感染和慢性感染的再次激活应使用捕捉法检测IgM,间接法检测IgM用于急性感染性疾病的诊断有待进一步探讨。     

桥梁养护管理系统的设计与开发

通过对桥梁养护数据管理、安全状态评估、养护辅助决策分析、信息共享等需求进行分析的基础上,应用Web-GIS技术和基于B/S网络结构技术对桥梁养护管理系统(BMMS)进行了设计,给出了系统结构、功能模块和养护评估模型;应用面向对象的软件开发技术研制了基于区域性管理的桥梁养护管理系统。将所设计的方案和研制的软件应用于江苏省汾灌高速公路桥梁养护的实际工程中,结果表明,桥梁缺损状况的评估结果与江苏高速公路工程养护有限公司技术工程师的评估结果一致,证实了所开发系统(BMMS)的可操作性,并实现了各个职能部门的信息共享和桥梁养护的区域性管理。     

路网环境下高速公路机电设备维护管理系统的研究

机电系统涉及通信技术、计算机技术、供电技术、控制技术、管理科学等多个领域,其管理技术难度较大,同时．系统设备发布在路网各路段沿线,其空间的分布性也给设备管理带来了很大的困难。因此,研究路网环境下高速公路机电设备维护管理的特点．利用先进的信息技术．建立基Intranet和GIS机电系统设备维护管理信息系统十分必要。     

基于均衡控制的双电源电动客车驱动系统设计

提出一种“B＋C”双电源纯电动客车驱动系统,即以能量型超级电容（C）为主要储能元件,辅以一定量的功率型锂电池（B）;根据车辆运行工况的特点,设计了相应的驱动系统均衡控制策略,通过Matlab/Simulink对系统及控制策略进行仿真分析。     

实验室教学管理系统的设计与应用

为了提高实验教学质量、管理水平和服务水平,加强实验室资源的开放与共享,针对实验室的实际情况,开发出了基于B/S架构的实验室教学管理系统。重点介绍了系统的规划和功能设计,该系统目前投入正常使用,效果较好。