机车轴端光电转速传感器优化设计

针对原有基于TB/T 2760—1999标准设计生产的光电式转速传感器在机车速度提高后不断发生故障的现象,采用高速编码器等新技术、新工艺、新材料对产品整体进行有针对性的优化设计,并对优化设计后传感器的总体结构和主要技术特点进行了介绍。试验验证以及在各机务段的批量装车考核表明,新产品性能优良,各项性能指标均很好地满足了设计要求。     

基于模糊约束的并联混合动力系统优化匹配研究

针对扭矩复合型式的并联混合动力电动汽车,在多能源系统能量控制策略确定的前提下,提出了以汽车性能要求模糊化和电池能量平衡为约束条件,对其动力传动系速比进行优化匹配以使燃油消耗达到最小。最后,通过一个具体实例车型加以验证和说明。     

交通量的灰包络预测

利用灰色预测法进行交通量预测有着满足贫数据序列,并可以处理波动明显的数据序列的优点。本文尝试运用灰色包络线预测的方法来预测交通量,可在预测远景交通量的同时,预测出其所在的区间,以保证预测的可信度。实践证明该方法具有较高的精度。     

蛋白质结构预测二维连续模型研究

利用模拟退火算法解NP完全问题的有效性，对新近提出的蛋白质结构预测问题的二维连续模型作了另解，并基于平面表达的清晰与美观将原模型作了相应的修改．最后，比较了模拟退火算法与原拟物算法的优劣，指出了另解具有的几个优点：去掉了物理背景，只作为一个纯数学问题来求解，有利于在计算机上实现；模拟退火算法收敛速度更快且精度更高。     

放射诱导p16基因胰腺癌靶向性表达的研究

目的　构建 pcDNA3.1 Egr.1p-p16重组质粒并检测其在人胰腺癌 JF305细胞中的辐射诱导表达。方法　将人p16 cDNA基因连接到有辐射诱导特性的早期反应因子 Egr.1p的下游,构建成 pcDNA3.1 -Egr.1p- p16 重组质粒,利用脂质体介导转染人胰腺癌细胞系 JF305 细胞;采用 RT- PCR方法和 Western blot方法检测不同剂量 X射线照射后,被转染细胞中 p16 的转录和表达水平。结果　酶切鉴定证实 pcDNA3. 1 Egr. 1p -p16 重组质粒构建正确。被pcDNA3.1 Egr.1p -p16重组质粒转染的人胰腺癌 JF305细胞经不同剂量 X射线照射后,p16 基因表达均高于未照射组。结论　X射线可诱导 pcDNA3.1 -Egr.1p -p16重组质粒在人胰腺癌 JF305细胞中表达增强。     

TGF-β1对成人骨髓CD105~+间充质干细胞增殖与分化的影响

目的分离并鉴定成人骨髓间充质干细胞(mesenchymalstemcells,MSCs),研究TGFβ1对骨髓MSCs增殖与分化的影响。方法分离成人骨髓单个核细胞;MACS磁珠分选CD105+细胞;FACS检测其免疫表型;诱导分选的CD105+细胞向脂肪及成骨细胞分化,油红O染色、VonKossa染色及RTPCR检测脂肪细胞及成骨细胞;观察TGFβ1对分选出的CD105+细胞分化与增殖的影响;免疫荧光染色检测诱导后的脂肪细胞CD105的表达情况;MTT法检测TGFβ1对CD105+细胞增殖的影响。结果成人骨髓来源的CD105+细胞体外可向脂肪及成骨细胞分化;在向脂肪细胞分化过程中,加入1～50ng/mLTGFβ1后,成脂诱导体系中无脂肪细胞出现;在向成骨诱导过程中,加入20ng/mLTGFβ1后,钙化基质沉淀明显降低;在脂肪诱导体系中,MTT染色检测显示TGFβ1对CD105+细胞的增殖有明显促进作用(P<0.05)。结论TGFβ1抑制成人骨髓源CD105+MSCs向脂肪及成骨细胞分化,在向脂肪诱导过程中,TGFβ1促进MSCs的增殖。     

含串补电容电气化铁路馈线故障测距算法

含串联补偿电容的电气化铁路牵引电网线路的电抗一距离关系不是单调的，按常规的电抗查表测距法，每个故障点会对应2个或多个定位点．通过对含串补电容馈线故障模型特性的分析，提出了一个新的判别方法．对不同的故障角和过渡电阻的ATP仿真以及MATLAB程序分析结果验证，该方法能够判断出故障与区间串补电容的相对位置．给出了含2个串补电容的电气化铁路馈线故障分段查表测距算法．     

电控发动机故障诊断技术

从分析电控发动机故障排除的基本原则及故障诊断的基本步骤与方法入手,通过对故障征兆模拟试验的举例,对常见故障诊断方法与技巧进行论述。     

ARTIFICIAL IMMUNE ALGORITHM OF MULTICELLULAR GROUP AND ITS CONVERGENCE

Objective To find out more extrema simultaneously including global optimum and multiple local optima existed in multi-modal functions. Methods Germinal center is the generator and selector of high-affinity B cells, a multicellular group＇s artificial immune algorithm was proposed based on the germinal center reaction mechanism of natural immune systems. Main steps of the algorithm were given, including hyper-mutation, selection, memory, similarity suppression and recruitment of B cells and the convergence of it was proved. Results The algorithm has been tested to optimize various multi-modal functions, and the simulation results show that the artificial immune algorithm proposed here can find multiple extremum of these functions with lower computational cost. Conclusion The algorithm is valid and can converge on the satisfactory solution set D with probability 1 and approach to global solution and many local optimal solutions existed.     

THE CHANGE OF SERUM TUMOR NECROSIS FACTOR-α LEVEL AND ITS CLINICAL SIGNIFICANCE DURING THE TREATMENT OF CHRONIC HEPATITIS B WITH LAMIVUDINE

Objective To evaluate the effect of lamivudine on immunity of chronic hepatitis B by observing the sequential changes of serum TNF-α and HBV-DNA level. Methods 31 CHB patients with elevated serum ALT/AST level and HBVDNA level higher than 106 copies/mL were treated with lamivudine (100mg/day) for one year. The sequential serum samples, which were taken at the 0, 3rd, 6th, 12th month after initiation of therapy, were used to detect serum level of TNF-α and quantity of HBV-DNA respectively. Results ① The serum TNF-α levels were higher than normal value before treatment in all patients; ② At In the 3rd month of treatment, The the serum HBV-DNA level began to decline and became negative in the 54.9% of all patients. At the end of treatment, HBV-DNA was negative in 48.4% of all patients; ③ The decrease of TNF-α level was later than HBVDNA level drop. TNF-α level began to decline after 6 months treatment. At the end of treatment, TNF-α level was lower than that at in 6th month, TNF-α level returned to normal in the 38.7% of all patients; ④ The TNF-α level decreased significantly after 6 months treatment in the patients with ALT>80IU/L at the beginning of treatment. But in the patients with ALT≤80IU/L, the TNF-α level decreased just after 12 months treatment; ⑤ TNF-α level fell obviously and early in patients whose HBVDNA became negative at in the 3rd month. Conclusion Lamivudine can suppress the replication of HBVDNA quickly, and decrease TNF-α level in the serum TNF-α level. It suggests that lamivudine can modulate immune response directly or indirectly. The changes of serum TNF-α level may be used to evaluate the clinical efficacy of lamivudine.