基于遗传算法的重载与非重载去向开行方案比较研究

本文道德简要介绍重载运输在我国开行的现状，在此基础上建立一个重载列车去向模型。引进一个先进的算法－遗传算法求解模型，根据求得最终优化方案，比较普通直达列车和重载列车开行方案的不同，计算结果表明重载列车的开行应该重新调整现有编组计划，而不是仅仅加大原有开行方向上的编成辆数以实现重载。     

亚洲牛带绦虫成虫延伸因子-1基因的表达、纯化及免疫学分析

目的 对亚洲牛带绦虫成虫延伸因子-1(elongation factor 1,EF-1)基因进行克隆、表达和免疫学初步研究.方法 将亚洲牛带绦虫成虫EF-1克隆到原核表达质粒pET-28a( )中,在大肠杆菌BL-21/DE3中用异丙硫代-β-D半乳糖苷诱导表达,表达产物通过十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)进行鉴定,用镍离子金属螯合剂亲和层析柱进行纯化,用蛋白印迹法(Western blotting)进行免疫学分析.结果 PCR、双酶切及DNA测序结果 均表明重组质粒pET-28a( )-EF-1构建成功.重组蛋白可被感染了亚洲牛带绦虫患者血清和猪血清识别,表明其具有免疫反应性.结论 亚洲牛带绦虫成虫EF-1基因可在原核表达系统中获得具有免疫学活性的表达,为进一步研究该蛋白的功能奠定了基础.     

转K1型转向架组装工艺

介绍了转K1型转向架的结构特点和关键技术参数,着重分析了侧架导框的加工、侧架与支撑座板的组焊和转向架的组装工艺等。     

灰色线性回归组合模型在地面沉降预测中的应用

选择合适的沉降与时间的关系模型对于沉降预测而言是很重要的。根据地面沉降机理与曲线特征，对某数据序列进行了光滑性和指教规律性检验，使用线性回归和灰色模型的组合模型对该数据序列建模和预测，所得平均相对误差和均方差比值都优于GM（1，1）。研究结果表明，具有线性特征的沉降数据序列选用灰色线性回归组合模型进行短期预测效果更好。     

客运专线整孔箱梁预制裂缝控制措施

分析客运专线整孔箱梁裂缝产生的原因，阐述了控制裂缝的措施和施工过程中出现裂缝的处理方法，为今后客专900t整孔箱梁施工提供参考。     

Dynamics and fishery of the Peruvian hake: Between nature and man

Mean length in the catch of Peruvian hake (Merluccius gayi peruanus) declined drastically by 8 cm in a few months in 1992. The 1991–93 El Niño event marked the beginning of changes in bottom environmental conditions, that along with other changes in the ecosystem, were the cause of the disappearance of large sized old hake from the traditional fishing grounds and the invasion of these areas by high concentrations of small sized young hake, traditionally distributed in the southern areas. A misinterpretation of this change gave an optimistic perception of the state of the stock, leading to a large increase of the fishing fleet and therefore to an increase of the fishing pressure on this resource. Catch increased quickly to very high levels in 1996, the fishery indicators of relative abundance remained stable, while global abundance of hake was diminishing. The resource responded to these natural variations of environment and to the intense fishing pressure with changes in some aspects of its biology and ecology. The most remarkable was the decrease of the length and age at first maturity that led to spawning by 1 year old (19 cm) individuals in a species whose observed longevity was 14 years.In 2002 a total and indefinite fishery closure was established by the Peruvian government and a technical commission for the hake recovery was created, which carried out continuous monitoring of the stock. After a closure of 20 months the indicators showed signs of recovery and the fishery was reopened in 2004. However, after an encouraging beginning during 2004 and the first quarter of 2005, neither abundance nor age structure had improved by the end of 2005, and in fact were deteriorating by the start of 2006. The survival of the age 2 and 3 groups was very low and few 4+ years old individuals were observed. Several factors working at different time scales have been proposed to explain the status of hake. The impact of the fishery, variations in the abiotic environment, predation by the jumbo squid (Dosidicus gigas) and cannibalism were considered as potential causal factors of this decline.     

不同级配对沥青混合料矿料间隙率的影响

沥青混合料矿料间隙率过大或过小都会对沥青混合料的路用性能产生不利影响。矿料间隙率对沥青混合料强度、耐久性和高温稳定性有很高的敏感度，是沥青混合料设计的一个重要参数之一。主要研究了不同级配对矿料间隙率的影响，通过马歇尔正交试验分析了级配对沥青混合料AC-13矿料间隙率的影响，得出了矿料间隙率与贝雷法评价级配的参数的关系。     

地铁计算机联锁系统测试工装的研究

针对当前轨道交通铁路信号维修中任务重、要求高的特点,提出通过测试工装实现备件状态的实时掌握,减少更换模块出现的不确定现象,做到替换上就可以用。对联锁测试工装的功能和测试方法做了基本的阐述,对地铁轨道交通CBTC的整体测试工装的研制,提供一个样板。     

船坞工程防渗及止水工艺介绍

本文介绍了船坞工程施工中防渗及止水工程的工艺及防渗止水效果分析。经采用多种不同的防渗、止水结构,保证了船坞有良好的防渗、止水效果。     

基于“1+X”证书制度的汽车类专业人才培养模式创新研究——以新能源汽车技术专业为例

文章主要是在“1+X”证书制度视角下,以新能源汽车技术专业为例,将“1+X”证书制度运用到汽车类专业人才培养模式中,更有效地提高汽车类专业人才培养质量。基于“1+X”证书的人才培养方案创新是在已有的教学模式基础上,深化教育教学改革,构建实训考核平台,加强教师队伍建设,并在实训过程中重视理论与实践的结合,将学生的理论知识运用到实践中,从而培养真正优秀的新能源汽车专业人才。