公路特大型泥石流研究及防治新理念

作为我国一种严重的公路水毁类型，公路特大型泥石流灾害至今尚未解决，本文建构了该类泥石流研究及防治新理念，对于公路建筑物的安全与稳定、确保公路交通有序进行具有重要意义。研究认为，公路泥石流研究及防治的主要对象是具有大冲大淤性能、毁损作用强烈的特大型泥石流；必须遵循以泥石流发育机理研究为基础、防治技术研究为手段、防治工程研究为目的的研究原则；详细分析了公路泥石流水毁模式，概化分析了冲淤变动型沟谷泥石流的发育特性；开发了防治效果极佳的速流结构，运用运动状态函数法建构了速流结构设计理念，运用动力学建构了泥石流最大抛程计算理论。     

高压喷射注浆技术在基坑管涌处理中的运用

高压喷射注浆法是在注浆法的基础上，应用高压喷射技术而发展起来的一项新的地基加固方法。本文针对武汉市泰合广场基坑在开挖过程中形成管涌或流上而致使附近建筑物遭受严重破坏的问题，采用高压喷射注浆法施工工艺方法，杜绝了基坑壁管涌或流立现象，使基坑开挖和基础施工得以安全进行。     

城市公交线网优化的线性模型

为了对现有公交线网进行优化,有效利用现有交通资源,解决城市交通问题,运用系统科学的思想,通过对城市公交线网优化的主要内容、优化原则、优化目标以及约束条件的分析,兼顾考虑乘客出行时间、公交线网密度和公交企业的利益以及公交线网的布局对整个城市的交通系统的影响,提出了优化目标的函数表达式及相应约束条件的数学表达式,建立了公交线网优化的线性模型,并给出了运用逐步筛选法对所提出的数学模型进行求解的方法。     

预应力混凝土先简支后连续梁静、动力试验研究

简要介绍预应力混凝土先简支后连续梁1∶5模型设计、试验方法。根据弹性试验、开裂试验和破坏试验结果,研究分析该类结构的工艺特点、正常使用荷载作用下结构受力变形特征、结构特别是湿接缝截面的抗裂性、开裂区域裂缝发展分布规律以及结构极限承载力。在对模型梁体系转换前、后和先简支后连续梁破坏前、后动力测试与理论分析的基础上,校核模型梁的刚度、制作精度,最后初步探讨了先简支后连续梁的动力性能。     

双速搅拌工艺

根据传统二次搅拌理论,提出了混凝土双速搅拌工艺,介绍了双速搅拌工艺的原理及优点,并指出了该工艺的实现方案.该工艺的应用可降低生产成本,提高混凝土的强度和生产率.     

移动闭塞系统区间通过能力分析及参数计算

移动闭塞条件下“闭塞分区”呈现出“移动”和“长度变化”的特征,其区间通过能力的计算较固定闭塞系统更加复杂。本文建立了移动闭塞系统区间通过能力的数学模型,对区间通过能力与列车追踪运行速度、跟驰车距和列车性能等参数之间的相互关系进行了定性、定量分析,并就关鍵参数的计算方法进行了深入的讨论,对移动闭塞条件下的列车运行控制与行车组织有一定的参考价值。     

A data model applied to the knowledge-based ICAS

Image-guided computer aided surgery system (ICAS) contributes to safeness and success of surgery operations by means of displaying anatomical structures and showing correlative information to surgeons in the process of operation. Based on analysis of requirements for ICAS, a new concept of clinical knowledge-based ICAS was proposed. Designing a reasonable data structure model is essential for realizing this new concept. The traditional data structure is limited in expressing and reusing the clinical knowledge such as locating an anatomical object, topological relations of anatomical objects and correlative clinical attributes. A data structure model called mixed adjacency lists by octree-path-chain (MALOC) was outlined, which can combine patient's images with clinical knowledge, as well as efficiently locate the instrument and search the objects' information. The efficiency of data structures was analyzed and experimental results were given in comparison to other traditional data structures. The result of the nasal surgery experiment proves that MALOC is a proper model for clinical knowledge-based ICAS that has advantages in not only locating the operative instrument precisely but also proving surgeons with real-time operation-correlative information. It is shown that the clinical knowledge-based ICAS with MALOC model has advantages in terms of safety and success of surgical operations, and help in accurately locating the operative instrument and providing operation-correlative knowledge and information to surgeons in the process of operations.     

降钙素基因相关肽与内皮素受体A在兔实验性蛛网膜下腔出血后基底动脉的表达

目的探讨蛛网膜下腔出血(SAH)后脑血管内皮细胞降钙素基因相关肽(CGRP)及内皮素受体A(ETRA)的表达与迟发性脑血管痉挛(DCVS)之间的关系。方法日本大耳白家兔60只,随机分为SAH模型组25只,盐水组25只,穿刺组5只,正常组5只。采用枕大池二次注血法制作SAH模型,灌注处死后取基底动脉制成切片。进行CGRP及ETRA的免疫组化和HE染色,观察CGRP与ETRA的免疫表达和病理结构变化,并用图像分析方法测量血管周长,使用方差分析进行统计学检验。结果基底动脉周长在SAH后均发生明显缩窄,其中在5d时缩小最强烈。CGRP与ETRA免疫阳性标记颗粒在SAH组的基底动脉组织中均有表达,其动态变化规律为CGRP的表达在1h时最明显,在3、5d依次表达减少,5d达到最低点,7d后开始逐渐升高,10d时表达相对比较明显,但未能达到正常水平,CGRP在穿刺组、正常对照组和盐水组也有散在不规则表达。在正常组、穿刺组和盐水组的基底动脉内ETRA有表达;SAH组基底动脉内ETRA在3d组和5d组表现为高表达,以3d组为强,而其中血管内皮细胞表达最强烈,平滑肌也有表达,10d组的表达仍然较强。结论在SAH后脑血管内皮细胞CGRP和ETRA的表达呈现明显的动态改变,但二者的变化趋势并不同步;CGRP和ETRA在DCVS的发生发展中起重要的作用。     

刀豆蛋白A条件化培养基对体外培养的兔角膜内皮细胞生长的影响

目的研究刀豆蛋白A(concanavalin A,ConA)条件化培养基对体外培养的兔角膜内皮细胞(rabbit corneal endothelial cells,RCECs)生长的影响。方法采用揭取后弹力层及内皮细胞联合组织块法进行RCECs的体外原代培养,分别用含有体积分数为5%、10%、15%、20%ConA的活化大鼠脾细胞条件上清液的RPMI1640培养基进行RCECs原代培养,并用含10%(体积分数)胎牛血清(fetal bovine serum,FBS)的RPMI1640培养基作为对照。神经烯醇化酶免疫组织化学染色法进行细胞鉴定;噻唑蓝比色法观察各组RCECs生长的状况;SPSS11.0One-way ANOVA及LSD检验进行统计分析。结果成功进行了RCECs的体外培养,培养细胞内神经烯醇化酶阳性表达。ConA条件化培养基各组细胞增殖均高于对照组(P<0.001),且10%、15%ConA条件化培养基组的RCECs细胞数量明显高于其他各实验组(P<0.001)。结论ConA条件化培养基具有促进RCECs生长的作用,可作为培养辅佐剂。     

Effect of ABCA1-V771M polymorphism on plasma lipid levels and its relationship with coronary atherosclerotic heart disease

Objective To explore the risk association of ABCA1-V771M polymorphism with coronary heart disease (CHD) in Hart nationality in Northwest of China. Methods With case-control study, ABCA1-V771M polymorphism was detected in 204 unrelated Hart nationality people in Northwest of China, and all the subjects by coronary angiography were grouped into 106 cases and 98 controls. The genotypes and alleles frequency distribution of ABCA1-V771M polymorphisms were analyzed by PCR-RFLP analysis, and the clinical statistics of serum lipids were compared and its effects of ABCA1-V771M polymorphism on the plasma lipid levels and coronary atherosclerotic heart disease were analyzed. Results The genotypic frequencies of ABCA1-V771M polymorphism matched well under Hardy-Weinberg equilibrium (P>0.05), V and M allelic frequencies were 33.3% and 66.7%. In comparison with VV VM genotype carriers, MM genotypes carriers had much lower plasma levels of HDL-C (P<0. 001) and much higher plasma levels of TG (P<0. 05). M allelic frequency in CHD group was significantly higher than V allelic frequency (P<0. 05). M allele was related with more severity of atherosclerosis in the coronary artery than V allele (P<0.05). However, there was no obvious difference in the incidence of AMI among carriers with three genotypes of ABCA1-V771M polymorphism (P>0.05). Conclusion ABCA1-V771M polymorphism was not only associated with the plasma levels of HDL-C and TG, but also related to the susceptibility and severity of coronary atheroselerotic heart disease. Moreover, M771 allele appeared to be atherogenie among Han population in Northwest of China.