鸡白细胞介素2在毕赤酵母细胞中的表达

目的　利用Pichia酵母真核细胞表达系统表达重组鸡白细胞介素 2 (IL 2 ) ,为大量制备鸡IL 2奠定基础。方法　将ConA激活的鸡脾脏T淋巴细胞中扩增得到的我国地方品种萧山鸡IL 2基因 ,克隆入pPIC9载体中构建重组质粒。将重组质粒电转化毕赤酵母GS115感受态细胞 ,甲醇诱导蛋白质表达。结果　SDS PAGE和Westernblot均在 30ku处检测到特异条带。结论　表明重组鸡IL 2基因在毕赤酵母GS115中获得表达     

基质金属蛋白酶-9和CD_(44v6)表达对乳腺癌转移和预后的意义

目的　探讨基质金属蛋白酶 9(MMP 9)及CD44v6在乳腺癌组织中的表达及其与肿瘤转移、预后的关系。方法　应用免疫组化方法检测 80例乳腺癌及 10例乳腺增生病中MMP 9及CD44v6的表达 ,并用计算机图像分析系统测定表达的吸光度值 (A)。结果　乳腺癌MMP 9和CD44v6表达的吸光度值显著高于增生病。淋巴结转移与MMP 9表达有关、与CD44v6表达有相关趋势。MMP 9与PR有负相关性、与ER有负相关趋势。MMP 9与CD44v6间无相关关系。MMP 9表达的吸光度值在生存 3年以上组低于 3年以下组 ,与乳腺癌患者生存情况有负相关关系。结论　有MMP 9表达的乳腺癌 ,肿瘤的侵袭和转移发生较早、预后差 ,MMP 9表达的测定可作为乳腺癌浸润、转移的重要分子学标志     

集成USB Thumb驱动器的车载数据采集系统设计

介绍了一种先进的车载数据采集系统的设计方案。该系统采用片上集成全速USB 2.0接口的MC9S12UF32为核心,大容量NAND闪存芯片TC58DVM92A1FT00为存储介质,具有体积小、容量大、传输速度快的优点,已应用于某型电动汽车的数据采集中,工作稳定可靠,大幅提高了工作效率。     

Variability of the western Galician upwelling system (NW Spain) during an intensively sampled annual cycle. An EOF analysis approach

The key features of the western Galician shelf hydrography and dynamics are analyzed on a solid statistical and experimental basis. The results allowed us to gather together information dispersed in previous oceanographic works of the region. Empirical orthogonal functions analysis and a canonical correlation analysis were applied to a high-resolution dataset collected from 47 surveys done on a weekly frequency from May 2001 to May 2002. The main results of these analyses are summarized bellow. Salinity, temperature and the meridional component of the residual current are correlated with the relevant local forcings (the meridional coastal wind component and the continental run-off) and with a remote forcing (the meridional temperature gradient at latitude 37°N). About 80% of the salinity and temperature total variability over the shelf, and 37% of the residual meridional current total variability are explained by two EOFs for each variable. Up to 22% of the temperature total variability and 14% of the residual meridional current total variability is devoted to the set up of cross-shore gradients of the thermohaline properties caused by the wind-induced Ekman transport. Up to 11% and 10%, respectively, is related to the variability of the meridional temperature gradient at the Western Iberian Winter Front. About 30% of the temperature total variability can be explained by the development and erosion of the seasonal thermocline and by the seasonal variability of the thermohaline properties of the central waters. This thermocline presented unexpected low salinity values due to the trapping during spring and summer of the high continental inputs from the River Miño recorded in 2001. The low salinity plumes can be traced on the Galician shelf during almost all the annual cycle; they tend to be extended throughout the entire water column under downwelling conditions and concentrate in the surface layer when upwelling favourable winds blow. Our evidences point to the meridional temperature gradient acting as an important controlling factor of the central waters thermohaline properties and in the development and decay of the Iberian Poleward Current.     

深水板桩码头超长钢管-板桩结合墙设计与施工技术

结合某挖入式港池工程,介绍深水整体卸荷式板桩结构的超长钢管-板桩结合墙设计及关键施工工艺。根据辅桩适应变形的能力,对主桩垂直度允许偏差提出0.4%的要求。通过对导向架合理的设计和改造,采用整桩起吊、振打结合、交错下沉的沉桩工艺等措施,确保顺利沉桩。结果表明,全部钢管-板桩结合墙都达到设计高程,沉桩效果良好,可为类似工程提供技术参考。     

白藜芦醇对胃癌细胞增殖的影响

目的 观察白藜芦醇(resveratrol,Res)在体外对胃癌细胞系BGC823增殖及其基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)表达的影响,初步探讨Res抑制胃癌细胞BGC823侵袭转移的机制.方法 应用不同浓度(10、50、100μmol/L)的Res分别作用于人胃癌细...     

散癖平胃方对人胃癌细胞SGC-7901侵袭转移的影响

目的观察散癖平胃(SPPW)方对人胃癌细胞SGC-7901侵袭转移能力的影响。方法 SPPW方含药血清干预人胃癌细胞SGC-7901 48h后,应用黏附实验、侵袭实验及迁移实验,检测SPPW方对人胃癌细胞SGC-7901侵袭转移能力的影响。应用实时定量聚合酶链反应(real-time PCR)检测SPPW对与侵袭转移有关的MMP-2和MMP-9mRNA表达的影响。结果 SPPW方各组含药血清干预人胃癌细胞48h后,SGC-7901细胞黏附抑制率、侵袭抑制率及迁移抑制率均增加且呈剂量依赖关系,与阴性对照组比较有统计学差异(P<0.01或P<0.05)。SPPW方三个剂量组对MMP-2mRNA的表达无统计学差异,对MMP-9mRNA的表达具有统计学差异(P<0.01)。结论 SPPW方可抑制人胃癌细胞SGC-7901黏附、侵袭和迁移能力,其抑制有明显的剂量依赖性,其抑制的机制可能与下调MMP-9的表达有关。     

基于MC9S12X的CAN通讯的底层代码设计

CAN总线因其高实时性能、高可靠性、稳定的传输性能、高抗电磁干扰性,使得它在汽车业中应用非常普遍。MC9S12X是一款高性能的16位处理器,可作为整车控制器的MCU,带有5个CAN通讯模块,可实现多路CAN通讯。论文给出了CAN底层代码的设计方法,并验证了代码,实现了MC9S12X与CAN助手之间的通讯,具有一定的实用价值。     

船舶CAD／CAE系统面向对象数据库的开发

针对关系型数据库在处理船舶设计复杂工程数据方面的不足,提出了采用面向对象数据库构建船舶设计数据管理系统基础平台的优越性,系统地介绍了船舶CAD/CAE系统面向对象数据库的设计及在Oracle 10g数据库上的实现方法,基于对象,关系数据库Oracle 10g采用JSP技术建立了B/S结构的船舶CAD/CAE数据管理系统为船舶CAD/CAE数据在Web上交互、共享和存储提供了一种有效途径.     

Oracle中实现定时任务的分析与应用

对Oracle Job Queue进行介绍与分析,提供在Oracle中实现定时任务的步骤和方法,并通过一个应用实例进一步加以阐述,对Oracle定时任务异常时的现象及处理办法进行总结.