Relationship between the expression of matrix metalloproteinase-9 and angiogenesis in glioma and its clinical significance

Objective To explore the role and significance of matrix metalloproteinase-9 (MMP-9) in angiogenesis through observing the relationship between the expression of MMP-9 and microvessel density (MVD) in glioma. Methods The expressions of MMP-9 and CD34 in 10 cases of normal brain tissues and 58 cases of glioma (14 cases of grade Ⅰ , 20 cases of grade Ⅱ , 15 cases of grade Ⅲ, and 9 cases of grade Ⅳ ) were detected by immunohistochemical streptavidin-peroxidase technique. The positive cells of MMP-9 and the positive microvessels were examined under binocular light microscope. Results The positive expression of MMP-9 in glioma was located in the tumor-cell cytoplast and endothelial cells. The positive rate of MMP-9 in glioma of grade Ⅰ, Ⅱ, Ⅲ and Ⅳ was 42.9%, 65.0%, 86.7% and 88. 9%, respectively. The expression of MMP-9 was obviously higher than that of normal brain tissues (P<0.01) and positively correlated with glioma malignancy (rz =0. 597, P<0.05). MVD was correlated with glioma malignancy (H=47. 865, P<0. 05). The expression of MMP-9 was significantly correlated with MVD (rz =0.897, P<0.01). Conclusion The expressions of MMP-9 and MVD are correlated with glioma malignancy, which may be helpful in judging the malignancy, invasion and prognosis. MMP-9 plays an important role in angiogenesis of glioma and accelerates glioma malignancy development by promoting angiogenesis.     

使用VB调用oracle数据库存储过程的研究

使用VB与oracle进行连接是软件开发人员经常使用的组合,充分利用oracle中的存储过程来处理应用程序频繁使用的逻辑,不仅可以提高效率和安全性,而且也可以最大限度地利用资源,减少编程人员的重复劳动。对存储过程的使用方法,重点对参数的传递、返回值的处理进行了分析,并举例说明了实际的处理过程,可以更好理解和使用存储过程解决实际问题。     

铁道部货调系统Oracle数据库的设计与实现

重点描述作为软件设计及开发者应该掌握的数据库优化技术,其中包括数据库数据对象的设计及编写SQL语句应该注意的问题,并结合铁道部贷调系统数据库的实现进行说明.     

Oracle数据库健康检查与建议

针对Oracle数据库健康检查,本文给出了一套较为科学全面的健康检查方案,并分别给出相关建议.     

DF9型客运内燃机车电传动系统改造设计

简要介绍了DF9型客运内燃机车运用情况,详细介绍了机车改造以后电传动系统的主要技术参数及特点。改造后的机车经过了负载试验及正线试运的考核,目前运用情况良好。     

一种基于ARM9的多路差分信号采集系统

文章针对多路差分信号采集问题,提出了一种基于ARM9的高精度采集系统。该系统通过采用I/O模拟SPI接口工作时序的方法,把ADC纳入ARM9的存储管理;通过将差分信号转换为单端信号,简化了整个系统设计。通过比较采集软件实际接收数据值和从采集的SPI时序图读出的采样数据值,得出CPLD控制逻辑和采集软件设计符合要求以及单次采样误差小于2%。     

中药治疗后MMP-9、TIMP-1及CA125在异位和在位子宫内膜中的表达

目的 探讨中医药治疗子宫腺肌症的作用机制.方法 37例行全子宫切除术的子宫腺肌症患者术前分为中药治疗组(n=17)和对照组(n=20).子宫内膜作为在位内膜标本,腺肌瘤作为异位内膜标本.采用免疫组化法测定并比较在位和异位内膜中基质金属蛋白酶-9(MMP-9)和组织金属蛋白酶抑制剂-1(TIMP-1)的表达水平,并观察两组患者治疗前、后血清CA125的变化.结果 中药治疗组异位内膜组织中,MMP-9在腺上皮细胞内的表达显著低于对照组(P<0.05),TIMP-1的表达显著高于对照组(P<0.05),MMP-9/TIMP-1比值显著低于对照组(P<0.05).中药组治疗后血清CA125 明显降低,与治疗前及对照组比较差异均有统计学意义(P<0.05).结论 中药可能通过下调MMP-9/TIMP-1的比值及降低血清CA125水平来控制异位内膜组织的种植侵袭.     

Construction of expression vector for NT4-ADNF-9 fusion gene

Objective To construct the prokaryotic expression vector bearing fusion gene NT4-ADNF-9 and lay foundation for further study on genetic therapy of neuraseusory deafness. Methods By means of asymmetrical prince/ template, double stranded eDNA of activity dependent neurotrophic factor-9 (ADNF-9) was obtained, which included restriction enzymes sites on the two extremities. ADNF-9 eDNA was ligated to the signal and leader peptides of nenrotrophin 4 (NT4), and the fusion gene was named NT4-ADNF-9. Then it was suheluned into prokaryotic expression vector pBV220, and called pBV220/ NT4-ADNF-9. Results Evidences of DNA sequence analysis and restrtction enzymes digestion showed that we recombined ADNF-9 eDNA to the 3'terminal of the signal and leader peptides of NT4, and the fusion gene was subcluned into pBV220 successfully. Bioactivity of the products was proved that it could support the cell survival and neurite growth in the primary cultures of dorsal root ganglia (DRG) of embryonic day-8 cbicken neurons as compared to the control. Conclusion Prokaryotic expression vector pBV220/NT4-ADNF-9 can be constructed successfully and the bioactivtty is satisfactory.     

基于汽车ECU串口的代码下载与调试方法的实现

提出一种基于串口通信来实现对汽车电控单元ECU代码的下载和调试方法,以解决长期以来依赖BDM对ECU进行代码下载和调试过程中出现的诸多不便.以美国飞思卡尔汽车级微控制芯片HCS 9S12为研究对象,通过自主开发的调试软件实现了无BDM对ECU的代码下载与调试,实践证明该方法简单、可靠、成本低,有一定的应用推广价值.     

Na3La9B8O27:Ln3+(Ln=Eu或Tb)荧光体的制备与发光性能

采用高温固相合成技术、溶胶-凝胶法、助熔剂法制备了Na3La9B8O27：Ln^3＋（Ln=Eu或Tb）荧光体,确定了固相反应制备该荧光体适宜的组分re．g,j．分别测试、解释了Na3La9B8O27：Ln^3＋和Na3La9B8O27：Tb^3激发光谱和发射光谱．比较了不同方法制备的荧光体的发光效率,并对产生的差异进行分析和讨论,助熔剂法得到的荧光体发光效率最高．