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121.
A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase, equipped with a polyhistidine affinity tag, was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×108, and the specific activity was 2.9×1010 RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein, the ATP content of bacteria was 9.48×10-16mol/mL, and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together, our results provided a simple and efficacious method of the preparation of recombinant luciferase, which could be applied in the determination of bacteria via ATP bioluminescence. 相似文献
122.
分析了图像边界检测的区域对比度模糊增强算法的特点,通过实验验证了该算法的正确性和可行性,并指出了算法的一些不足之处和待改进的地方,提出了一种适用轮廓提取的新广义算子。 相似文献
123.
现如今汽车已经成为人们必不可少的代步工具,汽车的数量随之也越来越多,在汽车的行驶过程中,难免会出现故障之类的问题,因此汽车的维修工作也变的尤为重要,其维修的师傅必须具备丰富的工作经验以及理论知识,然后借助检测诊断技术来进行汽车的事故和问题故障的检测排查工作。本文围绕汽车的检测诊断技术以及该技术在汽车维修中的应用展开分析。 相似文献
124.
This article proposes an efficient multiple model particle filter (EMMPF) to solve the problems of traffic state estimation and incident detection, which requires significantly less computation time compared to existing multiple model nonlinear filters. To incorporate the on ramps and off ramps on the highway, junction solvers for a traffic flow model with incident dynamics are developed. The effectiveness of the proposed EMMPF is assessed using a benchmark hybrid state estimation problem, and using synthetic traffic data generated by a micro-simulation software. Then, the traffic estimation framework is implemented using field data collected on Interstate 880 in California. The results show the EMMPF is capable of estimating the traffic state and detecting incidents and requires an order of magnitude less computation time compared to existing algorithms, especially when the hybrid system has a large number of rare models. 相似文献
125.
126.
由于交通运输量的不断增大以及运输行业的恶性竞争等原因,导致货车超载现象越来越普遍,并已成为急需解决的问题。探讨交通运输超限治理的对策,对于完善科学而系统的超限管理体系具有一定的借鉴意义。 相似文献
127.
汽车自动变速器检测台的开发研究 总被引:1,自引:0,他引:1
介绍了一种可对汽车自动变速器性能进行全面测试的检测台,论述了其结构组成、功能特点和推广应用价值。 相似文献
128.
仿真测试是开展城市轨道交通信号系统相关技术研究的一种有效方法。利用商业虚拟仪器开发平台LabVIEW,讨论了车载ATP(列车自动防护)设备仿真检测系统研制的技术和方法。实践证明,该车载ATP设备仿真检测平台具有高效、灵活、低成本的特点。 相似文献
129.
为了扩展无位置传感器无刷直流电动机(BLDCM)间接位置检测方案的适用速度范围.在电机采用三相六拍两两导通的条件下,基于对磁链相关函数的分析,提出了一种简化的磁链关系函数法。根据电机的数学模型和磁链关系函数,对电机本体和检测算法建模仿真。仿真结果表明该算法能在低、高速时准确检测BLDCM的换相位置。 相似文献
130.
Jiuh-Biing Sheu Stephen G. Ritchie 《Transportation Research Part C: Emerging Technologies》1998,6(5-6)
In this paper, a new methodology is presented for real-time detection and characterization of incidents on surface streets. The proposed automatic incident detection approach is capable of detecting incidents promptly as well as characterizing incidents in terms of time-varying lane-changing fractions and queue lengths in blocked lanes, lanes blocked due to incidents, and incident duration. The architecture of the proposed incident detection approach consists of three sequential procedures: (1) Symptom Identification for identification of incident symptoms, (2) Signal Processing for real-time prediction of incident-related lane traffic characteristics and (3) Pattern Recognition for incident recognition. Lane traffic counts and occupancy are the only two major types of input data, which can be readily collected from point detectors. The primary techniques utilized in this paper include: (1) a discrete-time, nonlinear, stochastic system with boundary constraints to predict real-time lane-changing fractions and queue lengths and (2) a pattern-recognition-based algorithm employing modified sequential probability ratio tests (MSPRT) to detect incidents. Off-line tests based on simulated as well as video-based real data were conducted to assess the performance of the proposed algorithm. The test results have indicated the feasibility of achieving real-time incident detection using the proposed methodology. 相似文献