Identification of serum biomarkers for diagnosing stage I lung adenocarcinoma by MALDI-TOF mass spectrometry

Objectire To identify specific biomarkers that could improve early diagnsis of lung adenuearcinoma using matrix-assisted laser desorptian/ionization (MALDI) technology. Methods Serum samples were isolated from 17 patients with stage I lung adenuearcinoma and 17 age- and sex-matched healthy controls, and the serum proteomic profiles were obtained by matrix-assistcd laser desorption ionization time of flight (MALDI-TOF) mass spectrometry. Results Compared with healthy control group, two highly expressed potential biomarkers were identified with the relative molecular weights of 6 631.64 Da and 4 964. 21 Da. The two best novel protein peaks were automatically chosen for the system training and the development of the constructed model. The constructed model was then used to test an independent set of masked serum samples from 15 lung adenocarcinoma patients and 22 healthy individuals. The analysis yielded a sensitivity of 93.3 %, and a specificity of 95.5 %. Conclusion These results suggest that MALDI-TOF-MS ProteinChip technology is a quick, convenient, and high-output analyzing method that is capable of selecting several relatively potential biomarkers from the serum of lung adenocarcinoma patients and may have a clinical value in the future, and will provide clues to identifying new serologic btomarkers of lung adenocarcinoma.     

佛山平胜大桥锚跨大体积混凝土施工

佛山平胜大桥是一座独塔四索面混合梁自锚式悬索桥,文中主要介绍锚跨大体积混凝土施工及其温控措施。     

大扭矩范围制动器动态试验台的设计

本文介绍了一种大扭矩范围制动器动态试验台的试验原理,阐述了试验台的设计依据,以及飞轮组转动惯量的确定与分配方法,并用实例详细说明设计过程.     

土木工程结构振动控制技术及其应用研究

结合工程实际,介绍了结构振动控制方法,并研究液体质量双调谐阻尼器(TLMD)及杠杆质量减振器(LMD)等新型阻尼减振系统.室内及实桥试验表明:由于兼具调谐质量阻尼器(TMD)与调谐液体阻尼器(TLD)的优点,TLMD便于调谐,减振效果与耐久性更好.理论及室内试验证明:与现有减振措施相比,LMD对桥梁景观的不良影响小,易于安装、养护,且减振效果更好,是一种更适于超长斜拉索振动控制的"广谱"减振器.     

节理岩体中纵向间距对连拱隧道稳定性的影响

为了研究双连拱隧道左右幅开挖对临洞的影响,以云南省晋红高速公路蔡官营隧道为研究对象,采用块体离散元软件3DEC中的黏结块体模型建立数值模型,模拟分析双连拱隧道一侧开挖对临洞拱顶沉降和围岩节理破坏范围的影响。研究结果表明：该模型能够合理地表征蔡官营隧道纵向间距过大或过小时现场出现的异常变形及结构破坏等现象;开挖过程中,无论是先行洞或后行洞开挖都会造成临洞拱顶沉降值增大及围岩中张拉、剪切破坏节理数量的增加,且破坏的节理主要以剪切滑动破坏为主,说明除了位移、应力外,节理破坏数量的变化也可作为围岩所受扰动大小的判断依据。然后以多个指标为判断依据,研究该隧道左右幅掌子面不同纵向间距对隧道围岩及支护结构的影响,进而确定合理的纵向间距;根据先行洞掌子面附近拱顶沉降值变化得出合理间距不小于30 m,根据张拉失稳节理面的数量得出合理间距为20~40 m,根据处于滑动状态的节理数量得出合理间距为30~40 m,根据发生滑动节理数总和得出合理间距为20~40 m,根据中隔墙的水平位移得出合理间距为20~40 m,根据二衬弯矩值得出合理间距不大于40 m,综合所有判断依据,得出隧道左右幅掌子面纵向间距应介于30~40 m（隧道净空的2~2.5倍）之间。在后续施工过程中,将左右幅掌子面纵向间距严格控制在该范围内,最终隧道顺利实现了贯通。     

Dynamics of an ultra-deepwater mooring line with embedded chain segment

A code is developed to study the mooring tensions on a line with an embedded segment considered, based on lumped mass method. The accuracy of this code is validated by published results. The influence of soil-chain interaction on the static and dynamic behaviour of a 1500 m mooring line is studied under two operational situations: shallow embedded taut mooring line and deep embedded semi-taut mooring line. The shallow case has an embedded depth setting as 10 m under seafloor, while the embedded depth is 20 m for deep water. To study the influence of soil on mooring dynamics, three simplified cases without soil force considered are investigated and the results are compared with the dynamics of embedded mooring. These three cases include: i) Case MA has the same spread distance to embedded mooring anchored on seafloor; ii) Case MB is anchored on seafloor and it has the same pretension with embedded mooring; iii) Case MC is anchored to the same point to the embedded mooring but the soil force is not considered. Furthermore, the effects of effective width parameters of tangential and bearing soil resistance on mooring dynamics are also discussed. In this work, three different oscillations are forced on fairlead to study the mooring dynamics, which are low frequency oscillation, hybrid oscillation (low frequency and wave frequency) and wave frequency oscillation.     

浅覆土砂层土压平衡盾构始发掘进对环境的影响分析

盾构始发和到达安全掘进控制技术是困扰盾构隧道施工的主要难题之一。盾构进出洞施工中,面临掌子面失稳、土体坍塌、涌水涌砂、地表沉陷、盾构"抬头及磕头"等诸多施工风险。本文从基坑及隧道的破坏模式,塑性区分布范围出发,通过有限差分法针对易受扰动的浅埋砂层地层盾构始发段进行了研究,为加固区范围大小的选择提供了依据。研究表明,在不采取加固措施时,基坑开挖后隧道始发段的竖向位移、拱底的隆起和最大纵向挤出变形量均过大,显示基坑将发生坍塌等破坏。当盾构始发段主要加固措施为旋喷桩,加固后的土体的物理参数得到了显著的提升。本文在此基础上得出了旋喷桩的合理加固区域。     

OPTIMUM PARAMETERS AND DESIGN OF TUNED MASS DAMPER FOR BUILDING FRAMES UNDER GROUND ACCELERATION

IntroductionTuned mass damper(TMD) was proposed byOrmondroyd and Den Hartog[1] and was studied bymany researchers[2～ 7] .TMD is effective in reduc-ing the wind- induced response of structures.Fur-thermore,TMDs have been successfully installedin many actual building such as the2 4 4 m highJohn hancock tower and the 2 80 m high Citicorpcenter office building in the mitigation of the wind-induced excessive vibration.For seismic applica-tions,however,there has not been a generalagreementon …     

城市出租车行业群发性事件风险预警方法

为实现对城市出租车行业的实时监测和预警,避免或减轻出租车行业群发性事件造成的不良影响,从出租车市场供给需求指标、市场价格与经营成本指标、市场经营环境指标、相关政策指标四个方面构建出租车行业群发性事件风险评估指标体系,利用层次分析法和加权平均法建立相应的风险评估模型,提出风险预警级别划分标准,并采用累积频率曲线法确定各风险等级的阈值。基于安徽省出租车行业近年来的出租车群发性事件对模型进行了标定,并以安徽省蚌埠市为例进行了应用验证,结果表明本文提出的方法具有较好的实用性,可实现对城市出租车行业群发性事件的风险预警和风险源的识别。     

高压旋喷法处理高速公路软土地基

结合实际工程,介绍了高压旋喷注浆法加固高速公路软土地基,叙述了其在处理公路软弱地基工程中的设计计算过程、施工工艺并分析了加固效果,,证明这种加固方法可以应用于高速公路的软土路基加固工程中.