太行山隧道膏溶角砾岩地段合理断面型式研究

石太客运专线太行山隧道膏溶角砾岩地层,围岩松动压力和膨胀压力大。对圆形、马蹄形、椭圆形三种断面型式进行计算、分析对比,提出膏溶角砾岩地层区段隧道的合理断面型式。     

EFFECTS OF ANTISENSE OLIGODEOXYNUCLEOTIDES ON EXPRESSION OF CASPASE-3 IN Γ-RADIATION INDUCED APOPTOTIC HL-60 CELLS

Objective To study the inhibitory effects of caspase-3 mRNA antisense oligodeoxynucleotides (ASODN) on expressions of caspase-3 and it's mRNA in γ-radiation induced apoptotic HL-60 cells, and screen the effective ASODN. Methods ASODN-1 and ASODN-2 targeting 5′-noncoding region and initial translation region of caspase-3 mRNA were respectively designed, synthesized and introduced into HL-60 cells by means of liposome-mediated transfection followed by 10Gy γ-radiation exposures. TUNEL assay was conducted to investigate the morphologic change and apoptotic percentage of HL-60 cells 18 h later. Immunocytochemical staining and one step RT-PCR were respectively performed to detect the expressions of caspase-3 and it's mRNA. Mismatched oligodeoxynucleotide (MODN) transfected and un-transfected HL-60 cells were taken as control. Results TUNEL assay found that the apoptotic percentages in ASODN-1 and ASODN-2 groups were significantly reduced compared with the control groups (P<0.01) when the final concentration of both ASODNs was ≥3μmol/L. Immunocytochemistry showed that caspase-3 positive cell percentages were reduced but the average gray values increased significantly compared with the control groups (P<0.01). RT-PCR showed expressions of caspase-3 mRNA was decreased after ASODN transfection. Furthermore, ASODN-1 proved more effective in inhibiting HL-60 cell apoptosis than ASODN-2 (P<0.01). Conclusion Caspase-3 mRNA ASODNs can prevent HL-60 cells from apoptosis induced by γ-radiation and reduce expression of caspase-3 and its mRNA. These effects are dose dependent in a certain range.     

君威轿车本土化快速自主开发及其关键技术

泛亚汽车技术中心在多年引进消化国际先进技术基础上,以中高级轿车产品为突破点,集中对市场分析与本土化产品定义、产品平台的再次开发、多品种混线生产与垂直换型、数字化设计与实验分析、产品开发过程的全球协同管珲等5个方而的火键技术进行了研究和开发,形成了完整的中高级轿车本土化快速自主开发平台、技术能力和管理体系,并成功应用到君威系列轿车开发中,使合资企业在自主开发上开拓出新的途径。     

Study on Performances of Car-following Models Induced by Motions of a Leading Car

IntroductionReasonably accurate mathematical modeling oftraffic engineering is needed not only for better un-derstanding of the collective behavior of traffic,but also for analyzing flow conditions, designingefficient control strategies, and etc[1]. For the pastfew decades, many researchers made studies of thissubject and a wide range of traffic flow theoriesand models have been developed. According to thelevel-of-detail with which the traffic flow is de-scribed, these traffic models were clas…     

比例延迟微分方程线性多步法的散逸性

考虑了比例延迟微分方程的数值方法的散逸性,把一类线性多步法应用到以上问题中,得到了该数值方法的散逸性结果。     

道路红线可视化技术研究

随着计算机图像处理软件（Boujou、Adobe Illustrator、Adobe After Effects、Adobe Premiere Pro）、无人机技术的飞速发展,在道路设计过程中,可视化技术得到了大量的应用,在很大程度上提升了传统设计的效率和质量。例如在道路前期方案设计中对道路红线可视化技术的应用探索,就是借助无人机航拍技术,利用上述计算机图像处理软件,在航拍的视频或图片中将规划红线绘制出来,从而更好地展示项目的规划范围、用地拆迁等,更加真实和直观地展示项目建筑拆迁、相交道路等信息。     

城市轨道交通车站配线的研究

以列车运行的安全性、准时性、捷达性和经济性为出发点,指出车站配线的基本目的是为列车调度运行服务,提高列车调度机动性,灵活解决实际运行中的多种状态和功能需求。根据实际运营的需求和不同的功能要求,车站配线可以分为折返线、待避线、停放线、渡线、联络线、支线接入线、车辆出入线和安全线等8类。车站配线的设置必须从线网布局、车站分布、客流断面分布特征等全局考虑,并对客流预测、运行交路、发车密度、停站时分、列车满载率等基础要素进行分析。通过分析典型图例,研究了配线的分类、站点与配线的分布、配线的形式之间的相互关系,总结了配线的规则以及配线设置中需要注意的问题。     

铁路客运专线与城市交通的衔接及站点选址评价方法研究

分析了铁路客运专线与城市交通的衔接体系的组成要素,提出了铁路客运专线与城市交通衔接的具体目标,运用层次分析法建立了铁路客运专线车站选址的评价指标体系和评价模型.     

焊装手动线自动化柔性改造方案

焊装车间手动生产线工人较多,用工成本高,且零件焊接质量一致性受工人影响;本文介绍一种手动生产线利旧夹具改造为自动化柔性焊接岛的方案;改造后只需取放件工人,降低了人工成本并提高了焊接质量一致性.同时,焊接岛可多车型批量切换生产,并为后期升级预留接口.     

城市轨道交通技术标准参数的AHP模糊综合评价

对城市轨道交通技术标准参数的评价,是进行方案选择的前提.基于AHP的模糊综合评判法这种工具为我们提供了这种途径.本文介绍了这种方法的基本理论和操作步骤,并通过实例分析,表明该方法的优越性和可行性.