亚洲牛带绦虫成虫延伸因子-1基因的表达、纯化及免疫学分析

目的 对亚洲牛带绦虫成虫延伸因子-1(elongation factor 1,EF-1)基因进行克隆、表达和免疫学初步研究.方法 将亚洲牛带绦虫成虫EF-1克隆到原核表达质粒pET-28a( )中,在大肠杆菌BL-21/DE3中用异丙硫代-β-D半乳糖苷诱导表达,表达产物通过十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)进行鉴定,用镍离子金属螯合剂亲和层析柱进行纯化,用蛋白印迹法(Western blotting)进行免疫学分析.结果 PCR、双酶切及DNA测序结果 均表明重组质粒pET-28a( )-EF-1构建成功.重组蛋白可被感染了亚洲牛带绦虫患者血清和猪血清识别,表明其具有免疫反应性.结论 亚洲牛带绦虫成虫EF-1基因可在原核表达系统中获得具有免疫学活性的表达,为进一步研究该蛋白的功能奠定了基础.     

机车乘务员出乘“三交三问”系统的开发与应用

介绍了机车乘务员出乘时“三交三问”培训、考试系统,系统有机地将“三交三问”培训考试、用户管理、题库管理、成绩查询、考试分析、数据备份与还原等模块集成在一起,充分利用了模块化的设计思想和开发方法,探索出机车乘务员班前针对性教育的新路子。     

转K1型转向架组装工艺

介绍了转K1型转向架的结构特点和关键技术参数,着重分析了侧架导框的加工、侧架与支撑座板的组焊和转向架的组装工艺等。     

内侧轴箱式转向架曲线通过性能研究

内侧轴箱式转向架是一种新型结构模式的转向架。文章通过动力学仿真软件对该结构转向架的曲线通过性能进行了计算,并在与传统转向架动力学性能对比的基础上,进一步研究了内侧轴箱式转向架的曲线通过性能。     

灰色线性回归组合模型在地面沉降预测中的应用

选择合适的沉降与时间的关系模型对于沉降预测而言是很重要的。根据地面沉降机理与曲线特征，对某数据序列进行了光滑性和指教规律性检验，使用线性回归和灰色模型的组合模型对该数据序列建模和预测，所得平均相对误差和均方差比值都优于GM（1，1）。研究结果表明，具有线性特征的沉降数据序列选用灰色线性回归组合模型进行短期预测效果更好。     

机车车轴疲劳问题分析与对策

通过对机车车轴常见故障及车轴的疲劳问题进行探讨,对车轴的疲劳裂纹、疲劳强度进行分析,提出了在机车车轴设计,材料、工艺选择以及运用维护中应采取的对策。     

内燃机车柴油机振动烈度评定方法探讨

从机车在"0位"时柴油机晃动现象入手,对"柴油机振动烈度评定方法"进行了分析探讨。重点对测量点、测量参数计算、评定准则进行了阐述,重新提出了"柴油机振动烈度评定方法",同时给出了振动位移的控制值。     

HXD2型大功率交流传动电力机车车体

介绍了HXD2型大功率交流传动电力机车车体的结构特点、参数、主要部件的结构设计.     

Dynamics and fishery of the Peruvian hake: Between nature and man

Mean length in the catch of Peruvian hake (Merluccius gayi peruanus) declined drastically by 8 cm in a few months in 1992. The 1991–93 El Niño event marked the beginning of changes in bottom environmental conditions, that along with other changes in the ecosystem, were the cause of the disappearance of large sized old hake from the traditional fishing grounds and the invasion of these areas by high concentrations of small sized young hake, traditionally distributed in the southern areas. A misinterpretation of this change gave an optimistic perception of the state of the stock, leading to a large increase of the fishing fleet and therefore to an increase of the fishing pressure on this resource. Catch increased quickly to very high levels in 1996, the fishery indicators of relative abundance remained stable, while global abundance of hake was diminishing. The resource responded to these natural variations of environment and to the intense fishing pressure with changes in some aspects of its biology and ecology. The most remarkable was the decrease of the length and age at first maturity that led to spawning by 1 year old (19 cm) individuals in a species whose observed longevity was 14 years.In 2002 a total and indefinite fishery closure was established by the Peruvian government and a technical commission for the hake recovery was created, which carried out continuous monitoring of the stock. After a closure of 20 months the indicators showed signs of recovery and the fishery was reopened in 2004. However, after an encouraging beginning during 2004 and the first quarter of 2005, neither abundance nor age structure had improved by the end of 2005, and in fact were deteriorating by the start of 2006. The survival of the age 2 and 3 groups was very low and few 4+ years old individuals were observed. Several factors working at different time scales have been proposed to explain the status of hake. The impact of the fishery, variations in the abiotic environment, predation by the jumbo squid (Dosidicus gigas) and cannibalism were considered as potential causal factors of this decline.     

关于互通选型与规模的几点认识

立交型式的选择是立交建设中重要的前期工作，型式不同，将使整个立交的交通功能、投资、景观及社会和经济效益等方面均受到影响。