β淀粉样肽1～42单克隆抗体的制备

目的　制备稳定分泌β -淀粉样肽 1～ 4 2 (Aβ1-42 )单克隆抗体的杂交瘤细胞 ,产生高效价抗体。方法　通过基因工程技术 ,将Aβ1-42 基因融合于乙肝核心抗原 (HBcAg)的主要免疫优势区 (MIR) ,制备Aβ和HBcAg的融合蛋白 ,免疫BALB/c小鼠 ,取脾细胞与SP2 / 0细胞融合 ,筛选能稳定分泌Aβ1-42 单克隆抗体的杂交瘤细胞。结果　得到两株能稳定分泌Aβ1-42 单克隆抗体的杂交瘤细胞 ,Aβ1-42 单克隆抗体的Ig亚类为IgG3 。结论　制备得到的Aβ1-42 单克隆抗体特异性强 ,效价高     

人脑胶质瘤nm23-H1蛋白及PCNA的表达

目的　研究人脑胶质瘤中nm2 3 H1蛋白和增殖细胞核抗原 (PCNA)表达状况。方法　采用免疫组化染色技术 ,对 5 3例人脑胶质瘤石蜡切片中nm2 3 H1蛋白和PCNA进行检测。结果　 5 3例人脑胶质瘤免疫组化染色结果显示 ,人脑胶质瘤I级、II级中nm2 3 H1阳性细胞显著高于Ⅲ、Ⅳ级 (P <0 .0 5 ) ;Ⅲ、Ⅳ级中PCNA阳性细胞显著高于I、II级(P <0 .0 5 ) ,但PCNA蛋白表达与nm2 3 H1表达无显著相关性。结论　nm2 3 H1蛋白低表达及PCNA高表达与脑胶质瘤的病理学分级相关 ;nm2 3 H1表达可能成为脑胶质瘤的浸润与转移的指标。     

一种具有模糊费用系数的VSP的修正C-W节约算法

将传统的确定性车辆调度问题扩展为具有模糊特征的模糊车辆调度问题．在对具有模糊费用系数的车辆调度问题进行简单描述的基础上，构建了模糊车辆调度的数学模型；通过Gaufmann-Gupta模糊数排序方法与传统车辆调度问题的C—W节约算法的有效结合，提出了解决该问题的一种改进C—W节约算法．最后，给出了一个算例．     

新扩展强化非对称选择网的有界性与单调性

根据Petri网结构理论，讨论非对称选择网(AC)的一个子类：新扩展强化非对称选择网(NESAC)的有界性与活性单调性，判定NESAC网N结构有界的充分必要条件是，N被极小死锁簇覆盖，每个非空极小死锁H一定是个陷阱且满足|t^*∩|=|t^*∩H|=1，NESAC网仍然具有ESAC活性单调性的特征。     

构建全省网管通信网络平台

为适应电信管理网络向集中管理、集中维护方向发展的趋势,建立数据通信网为现有各分散的网管系统(如电话交换网管、传输网管、支撑网管等)提供一个统一的数据通信网络平台。各网管系统的信息都可以在此数据通信网上进行传递,使数据通信网成为一个真正的综合数据通信网。其网络结构是采用以省为中心,辐射地、市的广域网络,形成双星形网络结构。     

基于实验结果的有机静电感应三极管动态特性

根据采用有机半导体材料酞菁铜制作的有机静电感应三极管的测试结果，建立适当的动态小信号模型，对其动态特性进行仿真计算，得出器件的截止频率．通过对截止频率的分析，提出改善有机器件动态特性的方法．     

A DISPATCHER TRAINING SIMULATOR SYSTEM INTEGRATED WITH THE EXIST SCADA／EMS

To utilize exist SCADA(Supervisory Control and Data Acquisition)/EMS (Energy Management System) fully and economize capital, Henan Electric Power Dispatching and Communication Center in China established a set of Henan Dispatcher Training Simulator (HNDTS) base on its exist SCADA/EMS. In order to integrated with exist SCADA/EMS, the integration method and technique are proposed. Graph data integration discussed with emphasis.After integration implemented, HNDTS can share all data with SCADA/EMS and dispatchers can be trained in same environment as real work situation, in the same time it can avoid amout of work of maintenance engineers. Both advantages and disadvantages of integration are analyzed. In the end of paper, the requirement for future DTS is put forward base on the experience of author.     

EXPRESSION OF NESTIN AND GLIAL FIBRILLARY ACIDIC PROTEIN IN DIFFERENT PERIOD AFTER SPINAL INJURY IN ADULT RATS

Objective To study the expression of Nestin and glial fibrillary acidic protein (GFAP) in different period after spinal injury in adult rats. Methods Animal moels were created by artery clamp. Expression of Nestin and GFAP in different period ( 1,3,5days; 1-8 weeks) and different area( injury locus and its surrounding tissue ) after spinal injury were observed pathologicaly using immunofluorescence and LeicaQ5001W imaging processing system.Results There was expression of Nestin and GFAP in injured area 1 day after injury. The expression increased not only in in injured area but its sourrounding 3--7 days later and gradually got to peak value. As the time went on, expression of Nestin and GFAP dereased. Conclusion Spinal injury can induce the expression of Nestin. Nerve stem cell has response to spinal injury. There is positive correlation between expression of Nestin and hyperplasia of reactivity astrocyte. Nerve stem cell maybe invovled in the repair of central nervous system (CNS).     

THE GENE EXPRESSION OF BDNF IN NORMAL RABBIT RETINA

Objective To investigate the distribution of brain-derived neurotrophic factor(BDNF) protein in the rabbit retina. Methods Immune response material in the retina was observed using BDNF antibody by the method of immunohistochemistry. Results BDNF gene expression was mainly found in the RGCs, also in innernuclei cells and outernuclei cells in rabbit retina. Conclusion RGC is not only the target cell of BDNF, but also express the BDNF protein. BDNF from multi-sources participates in the regulation of RGCs.     

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通过分析中铁快运创立以来成功拓展快递业务网络的案例，揭示了铁路传统运输体制存在的主要问题和改革方向。作者认为，中铁快运低成本地快速形成了网格状的空间组织形式，并通过产权关系的不断调整实现了对快递运输的全程控制，成为国铁系统中具有真正意义并充满活力的运输企业。铁路重组必须构建起能够自主经营、责权明确的企业组织，并更新运输理念，在进一步扩大和完善基本业务的同时大力促进附加增值业务的发展，使该行业由过去的“自我服务导向型”向“客户或市场需求导向型”转变。