含盐地层顶管润滑泥浆抗盐及抗渗性能试验研究

为解决高含盐地层盐侵顶管润滑泥浆造成滤失量过大、黏度下降、动塑比下降，导致管周摩阻力增大，影响顶管施工的问题，依托山东省黄水东调二期工程的地质勘察结果，选用凹凸棒土、HV-CMC等抗盐材料，通过配浆试验得到配浆水+5%加量凹凸棒土+5%加量膨润土+0.35%加量NaOH+0.8%加量HV-CMC的泥浆配方。采用抗盐试验和抗渗试验测试盐侵前后黏度、滤失量、抗渗性等指标，得到结论如下： 1）钙镁离子由于价位高、水化能力差，盐侵影响大于钠离子； 2）润滑泥浆的黏度随盐侵增大而表现出先迅速减小后趋于稳定的现象； 3）地层粒径对泥浆抗渗性影响很大，建议泥浆使用前增设原状土中的抗渗性测试。     

β2受体阻滞剂对胰腺癌细胞侵袭能力的影响及其机制

目的研究β2受体阻滞剂对胰腺癌细胞的增殖及侵袭转移能力的影响及其作用机制。方法β2受体特异性阻滞剂ICI118,551、广谱β受体阻断剂普萘洛尔和β1受体特异性阻滞剂美托洛尔干预胰腺癌细胞MIA PaCa-2和BxPC-3后,通过MTT分析、流式细胞术,细胞侵袭实验,Western blot和EMSA等技术阐明β2受体阻滞剂对胰腺癌侵袭能力的抑制作用,进一步检测核转录因子NF-κB和AP-1的活性及其下游相关分子VEGF、MMP-2、MMP-9和COX-2的表达。结果在优势浓度(100μmol/L)下:普萘洛尔、ICI118,551诱导胰腺癌细胞周期G1/S期阻滞和抑制增殖、侵袭效应强于美托洛尔(P<0.05);三种阻滞剂均可下调NF-κB和AP-1的活性,并降低其相关下游分子VEGF、MMP-2、MMP-9和COX-2的表达(P<0.05),普萘洛尔、ICI118,551对上述分子抑制率强于美托洛尔,对BxPC-3的抑制强于MIA PaCa-2细胞。结论β2受体阻滞剂通过下调核转录因子及其相关下游分子的表达而抑制胰腺癌细胞的增殖及侵袭转移能力。     

JAK激酶抑制剂AG490阻滞乳腺癌MCF-7的侵袭和迁移

目的 探讨JAK/STAT3和MAPK/ERK1/2两条信号通路的交联作用及其对乳腺癌MCF-7细胞侵袭迁移的影响机制.方法 在乳腺癌MCF-7中,分JAK酶抑制剂AG490未处理组和处理组,Western blot检测磷酸化和非磷酸化STAT3和ERK 1/2蛋白的变化,RT-PCR检测STAT3、ERK1/2 mRNA的变化,明胶酶谱法检测MCF-7分泌MMP-2、MMP-9的变化,同时应用Transwell小室对细胞侵袭迁移能力进行研究.结果 AG490处理MCF-7细胞后,磷酸化和非磷酸化STAT3和ERK1/2蛋白均减少,STAT3、ERK1/2 mRNA转录水平也下降,同时也观察到了AG490能使MCF-7分泌MMP-2和MMP-9减少,从而显著降低了MCF-7细胞的侵袭迁移能力.结论 AG490可阻断JAK/STAT3和MAPK/ERK1/2两条信号通路,抑制MCF-7细胞分泌MMP-2和MMP-9,从而抑制了其侵袭迁移.     

茚甲新对胶质瘤细胞体外侵袭能力的影响

目的 研究茚甲新对胶质瘤细胞体外侵袭能力的抑制作用.方法 通过MTT实验确定不会引起细胞毒性的茚甲新药物浓度,应用移行实验和浸润实验研究茚甲新对胶质瘤细胞移行及浸润能力的影响.结果 在一定的浓度范围内,随着茚甲新浓度的增加,胶质瘤细胞的浸润能力明显下降,并且与对照组相比有统计学意义(P＜0.05).但是,其浓度并不影响...     

Relationship between the expression of matrix metalloproteinase-9 and angiogenesis in glioma and its clinical significance

Objective To explore the role and significance of matrix metalloproteinase-9 (MMP-9) in angiogenesis through observing the relationship between the expression of MMP-9 and microvessel density (MVD) in glioma. Methods The expressions of MMP-9 and CD34 in 10 cases of normal brain tissues and 58 cases of glioma (14 cases of grade Ⅰ , 20 cases of grade Ⅱ , 15 cases of grade Ⅲ, and 9 cases of grade Ⅳ ) were detected by immunohistochemical streptavidin-peroxidase technique. The positive cells of MMP-9 and the positive microvessels were examined under binocular light microscope. Results The positive expression of MMP-9 in glioma was located in the tumor-cell cytoplast and endothelial cells. The positive rate of MMP-9 in glioma of grade Ⅰ, Ⅱ, Ⅲ and Ⅳ was 42.9%, 65.0%, 86.7% and 88. 9%, respectively. The expression of MMP-9 was obviously higher than that of normal brain tissues (P<0.01) and positively correlated with glioma malignancy (rz =0. 597, P<0.05). MVD was correlated with glioma malignancy (H=47. 865, P<0. 05). The expression of MMP-9 was significantly correlated with MVD (rz =0.897, P<0.01). Conclusion The expressions of MMP-9 and MVD are correlated with glioma malignancy, which may be helpful in judging the malignancy, invasion and prognosis. MMP-9 plays an important role in angiogenesis of glioma and accelerates glioma malignancy development by promoting angiogenesis.     

基于GIS的我国近海外来生物入侵时空演变数据库系统构建方案研究

海洋生物入侵已成为世界海洋生态环境面临的四大问题之一。我国目前针对海洋外来生物入侵的数据资料缺乏,且未形成时空分布的数据系统。本文通过文献查找、资料收集与历史数据整理,现场调研等多种手段,拟掌握我国近海外来生物入侵时空演变历史及现状,提出基于GIS软件的数据库系统的建设方案。本系统的建设可实现海洋入侵物种查询,外来入侵物种判定、不同时期下,外来生物入侵我国近海海域的发生地点、入侵种类、灾害发生时间、灾害影响的对比分析等功能。     

Hypoxia-induced enhancement of cell invasiveness in SMMC7721 hepatocellular carcinoma cells

Objective To explore the effects of hypoxia (1% O2) on the ability of cell invasiveness and expression of KAI1/CD82 in SMMC7721 hepatocellular carcinoma cells. Methods SMMC7721 hepatocellular carcinoma cells were cultured by hypoxia ( 1% O2) in vitro, and the ability of cell invasiveness was analyzed by cell invasion assay.Immunohistochemistry staining technique was used to evaluate the protein expression of KAI1/CD82. Results Cell invasion assay revealed that hypoxia enhanced the ability of invasiveness of hepatocellular carcinoma cells. In addition,KAI1/CD82 protein expression was positive in cultured SMMC7721 hepatocellular carcinoma cells, and it was located diffusedly in the cytoplasm and on the membrane. KAI1/CD82 protein expression was down-regulated when mediated by hypoxia; at the same time, it showed a time-effect relationship. Conclusion Hypoxia can enhance invasiveness of hepatocellular carcinoma cells. The down-regulation of KAI1/CD82 expression may play a certain role in those courses.     

脆性组氨酸三聚体蛋白和蛋白激酶Ca的表达及其与非小细胞肺癌侵袭、转移的相关性

目的 探讨脆性组氨酸三聚体（FHIT）蛋白和蛋白激酶Ca（cPKCa）在非小细胞肺癌（NSCLC）中的表达及其与NSCLC侵袭、转移的相关性。方法 采用免疫组化PicTure^TM通用型二步法，对41例NSCLC组织和11例肺良性病变组织行FHIT蛋白和cPKCn检测。结果 FHIT蛋白和cPKCa在NSCLC组织中的阳性表达率分别为48．8％和53．7％，在肺良性病变组织中的阳性表达率分别为90．9％和18．20％，均有显著性差异（P〈0．05）；COX比例风险模型生存分析结果显示，FHIT蛋白（P=0．020，RR=2．754）、cPKCa（P=0．022，RR=0．385）具有独立的预后意义。结论 FHIT蛋白和cPKCa表达与NSCLC的发展、侵袭及转移显著相关，可作为判断NSCLC临床预后的参考指标。     

高铁周界异物入侵感知与识别系统

针对高铁周界异物入侵监测和预警需求,提出了一种融合分布式光纤传感技术、视频联动技术和智能识别技术的分布式、多维度高铁周界异物入侵感知与识别系统。通过使用布里渊光时域分析(BOTDA)技术进行应变监测以及相位光时域反射(Φ-OTDR)技术进行振动监测,可有效捕捉异物入侵事件。同时使用人工神经网络(ANN)对报警信号进行大数据分析,可降低系统误报率。该系统的应用能够大幅提高高铁运营安全预警完备率和应急响应效率,具有重大社会价值。     

采空区回填体隧道冒顶塌方处理技术

以铜陵市五松隧道在施工中遇到的采空区回填体塌方处理为例,针对采空区回填体导致冒顶塌方的特点,从安全顺利的角度,通过临时环向竖向钢支撑加固未塌方段、套管法超前管棚超前预支护、水泥水玻璃注浆加固、预留核心土法开挖支护、回填轻质混凝土、换拱等技术,安全顺利地处理完该塌方,为今后类似工程提供一定的参考。