Electrocatalytic oxidation and determination of dopamine at a carbon ionic liquid electrode modified with nafion-L-aspartic acid composite film

The electrocatalytic oxidation of dopamine (DA) was studied by electrochemical approaches at a carbon ionic liquid electrode (CILE) modified with the composite film of nafion and L-aspartic acid (NL-CILE). The CILE was fabricated by replacing non-conductive organic binders with a room-temperature hydrophobic ionic liquid, 1-butyl-3-methyl-imidazolium hexafluorophosphate. The composite film of NL was used as matrix to adsorb DA and catalyze the oxidation of DA in phosphate buffer solution (PBS). The electrochemical response of DA was investigated at the NL-CILE, the traditional carbon paste electrode (TCPE), CILE and the nafion modified CILE (N-CILE) in 0.1M PBS (pH 7.4), respectively. The results showed the superiority of NL-CILE to N-CILE, CILE and TCPE in terms of provision of higher sensitivity, faster electron transfer and better reversibility. Under optimum condition, the oxidation peak current was rectilinear with DA concentration range from 0.1μM to 0.1mM, with a detection limit of 0.03μM (S/N=3) by differential pulse voltammetry. The proposed method was applied to determine DA in samples successfully.     

Analysis of ganciclovir and its related substances using high performance liquid chromatography and liquid chromatography-mass spectrometry methods

Objective High performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC/MS) methods were developed for the determination of ganciclovir and its related substances. Methods A Hypersil ODS2 column (4.6mm×250mm, 5μm) was used with a mobile phase of 0.02M potassium 1.0mL/min, and UV detector set at 254nm was used for monitoring the eluents. Results The method was simple, rapid, selective and capable of separating all related substances at trace level with a detection limit of 0.04μg/mL. It has been validated with respect to accuracy, precision, linearity, and limits of detection and quantification. The linearity range was 10.2-153.0μg/mL with r=0.9998. The percentage recoveries ranged from 96.7% to 101.6%, and RSD was 1.24%-1.96% (n=5). Conclusion The method was found to be suitable not only for monitoring the reactions during the process development but also for quality control of ganciclovir. For identification of related substances, LC/MS was used. The mainly related substances of ganciclovir active pharmaceutical ingredients (API) were determined as guanine, (1, 3-dioxolan-4-yl) methyl acetate, and diacetyl guanine.     

高液限土路堤病害机理分析及处治措施

高液限土土性较差,用于修筑路堤容易产生失稳、沉陷等破坏。本着安全有效地利用该类资源,节约换填好土所带来的经济损耗的目的,对高液限土路堤病害机理进行分析,提出利用高液限土修筑路堤的处治建议,以保证高液限土路堤的稳定性,并改善其变形特性。     

厦门市快速公交首期线路运营效果分析

本文在介绍厦门市快速公交概况的基础上,对厦门市BRT系统特征及优势进行了阐述,并且从客流量分析、BRT服务水平和乘客满意度三方面具体分析了BRT的运营效果。最后,针对规划和运营中存在的问题提出了相应的改进建议,如加大BRT专用道管理力度、尽快投入18米车型、加快高峰车辆周转、研究BRT升级轻轨策略、调整常规公交线路等。     

快速公交(BRT)服务水平评价研究

在普通公共交通服务水平评价体系的基础上,构建系统、科学、客观、实用的城市快速公交服务水平评价指标体系。基于几种常用的评价方法,运用灰色聚类法对快速公交服务水平进行全面、客观的综合评价,反映快速公交服务质量。结合实际,进行了快速公交服务水平的评价,提出了相应改进措施。     

苜蓿叶形枢纽互通设置集散车道的必要性论证

由于全苜蓿叶或相邻象限设置环形匝道的部分苜蓿叶互通存在距离很近的相邻进出口,互通内主线存在交织区且交织长度短,交织车辆易对主线直行车辆造成影响。为提高主线的通行能力与行车安全,经验上一般在存在交织区的主线设置集散车道。考虑设置集散车道后,互通的占地与工程造价会较大幅度增加,设计阶段需对设置集散车道的必要性进行论证。利用美国《道路通行能力手册》（HCM2000）中对高速公路交织区通行能力的计算方法,介绍了全苜蓿叶枢纽互通是否需要设置集散车道的论证过程与方法。     

基于遗传层次综合评价的绥化东枢纽互通方案比选

伴随我国社会经济的高速发展,对交通通行能力提出了更高的要求,致使公路、道路网交通压力不断增加,交通拥堵问题日益严重。修建互通式立交影响因素比较多,在分析互通式立交方案各种影响因素的基础上,通过借鉴现有的立交方案评价指标体系及专家的评议,建立了新的、更贴切、更合理的综合评价指标体系,在众多方案比选方法中挑选出一种更优秀的评价方法。运用遗传层次分析法结合专家打分来确定各项指标的权重并进行一致性检验,将此方法与模糊综合评价法结合,用于互通立交方案的比选工作。将对绥化东互通立体从位置调查、线形选择、桥梁布孔等多方面进行方案比选论证。针对互通式立体交叉在建设中具有建设周期较长、资金较高、交通绕行距离较长等特点,结合相交道路特征、交通流特征、节点类型等因素选择适合绥化地区的立体方案。     

基于分形理论的级配评价与抗车辙性能研究

文章基于分形理论,对常用的沥青混合料AC-20、AC-16、AC-13的微观结构进行了分析,建立了质量分形维数与级配走向的相关关系,探讨了粗细集料比例变化下不同级配分形维数对沥青混合料抗车辙性能的影响。     

重复荷载作用下高液限粘土路基永久变形特性分析

重复荷载作用下高液限粘土路基的永久变形对道路正常运行有较大影响。结合FALC3D软件,建立高液限粘土（路堤）及其水泥改良土（路床）的路基弹塑性本构模型,分析路基在不连续半正弦波荷载下产生的动力响应。分析结果表明：在重复荷载作用下土体的永久变形逐渐累积,随着加载次数增加,初始屈服面不断增大,最后趋近于最大加载面;在重复荷载作用下,土体变形在荷载作用初期变形较大,随着荷载重复次数的增加,变形速率逐渐减小;水泥改良后的高液限粘土路床具有抵抗变形能力。该研究对于高液限粘土地区的高速公路建设具有重要的理论意义和实际价值。     

两台精密水准仪的校验比较分析

采用两台相同型号的精密水准仪,通过两名操作人员变换精密水准仪位置,比较了水准仪的视准误差,并列出视准误差的校验方法,可供水准仪间校验结果参考。