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It is an important step in understanding a bio-logical activity to profile proteins and peptides incells and tissues[1]. Two-dimensional polyacry-lamide gel electrophoresis (2D-PAGE) is very use-ful method for separating complex protein mix-tures[2]. Because of high resolution, improved re-producibility of 2D-PAGE with immobilized pHgradients, it has become one of the standard proce-dures for proteome research. Proteomic analyses are now emerged in manyareas of plant biology, such as genet… 相似文献
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