Bacillus brevis DX01 Labeling by Green Fluorescent Protein Gene and Its Colonization in Rice Seedling Roots

Since the gfp cDNA was cloned from Aequoreavictoria[1], the green fluorescence protein (GFP)has attracted considerable attention as a marker/reporter system[2, 3]. In recent years, the greenfluorescent protein gene has been used as a markersystem for detection of marked cells in soil.Tombolini et al.[4]used a red-shift mutant to de-tect individual bacterial cells on the surface ofroots. They also investigated the stability of theprotein under nutrient starvation conditions. Theprotein was s…