FOLLICULO-STELLATE CELLS OFTHE RAT ANTERIOR PITUITARY RESPONDED TO ANGIOTENSIN Ⅱ BY INCREASING INTRACELLULAR CONCENTRATION

Objective The main purpose of this study was to investigate whether the folliculo-stellate cells (FSC) respond to angiotensin(Ang) Ⅱ by increasing intracellular free concentration ([]i) ,and where the o-rigin of mobilization is if that has occurred. Methods Pituitary cells in primary culture were prepared from male Wister rats(250g) by a conventional method and cultured in MEM supplemented with 4％ normal rat serum. Af-ter 2 days in culutre,cells were loaded with 1 μmol/L fura-PE3/AM for 1 h and subjected to a ment with Quanti Cell 700 system. Excitation wavelengths of 340 and 380 nm were selected by means of a computer-controlled filterwheel. Results The of FSC in the rat anterior pituitary was elevated by Ang Ⅱ. The eleva-tion of of FSC induced by 0. 1,1.0,10 and 100 nmol/L Ang Ⅱ was (56.33±6.18), (117.07± 36.07), (175.59 ± 40.01 ) and (216.02 ±11.52) nmol/L, respectively. The increase of of FSC induced by 100nmol/L Ang Ⅱ was not influenced by the medium without (0Ca),but significantly suppressed by thapsigargin (TG),an inhibitor of ATPase. The rate of responsive FSC to Ang Ⅱ (100 nmol/L) was 61.84％ which was obviously higher than that of pituitary endocrine cells (43.49％). Conclusion The present experiment demonstrates that the FSC in the rat anterior pituitary responds to Ang Ⅱ by increasing [which raises the possibility that Ang Ⅱ re-leased from either lactotrophs or gonadotrophs affects FSC through paracrine mechanism. The elevation of [induced by Ang Ⅱ presents a dosage-dependent relation, and is possibly because of the release of from an intra-cellular pool (s). Fashions of release are relative to the concentration of Ang Ⅱ. The results indicate that Ang Ⅱ functions as a paracrine factor among pituitary cells including FSC.     

Estimation method for railway passengers’ train choice behavior with smart card transaction data

Smart card systems have become the predominant method of collecting public transport fares in Japan. Transaction data obtained through smart cards have resulted in a large amount of archived information on how passengers use public transportation. The data have the potential to be used for modeling passenger behavior and demand for public transportation. This study focused on train choices made by railway passengers. If each passenger’s train choice can be identified over a long period of time, this information would be useful for improving the customer relationship management of the railway company and for improving train timetables. The aim of this study was to develop a methodology for estimating which train is boarded by each smart card holder. This paper presents a methodology and an algorithm for estimation using long-term transaction data. To validate the computation time and accuracy of the estimation, an empirical analysis is carried out using actual transaction data provided by a railway company in Japan. The results show that the proposed method is capable of estimating passenger usage patterns from smart card transaction data collected over a long time period.     

FOLLICULO-STELLATE CELLS OF THE RAT ANTERIOR PITUITARY RESPONDED TO ANGIOTENSIN Ⅱ BY INCREASING INTRACELLULAR Ca~(2 ) CONCENTRATION

Previous studies have shown that folliculo-stellate cells(FSC) in the adenohypophysis pos-sess various functions[1] .It has been most noticedthat FSC regulate the hormone secretion from pitu-itary endocrine cells by a paracrine mechanism orgap junction- mediated exchanges[2 ,3] .Studies usingpituitary cell aggregates enriched with FSC re-vealed that the cells mainly inhibit the release ofgrowth hormone- releasing hormone(GHRH) andβ- adrenergic stimulation of growth hormone (GH)release,t…