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排序方式: 共有108条查询结果,搜索用时 15 毫秒
1.
Daniel Baldwin Hess 《Transportation》2012,39(2):247-266
The walking trip from an origin or destination to a bus stop or transit station can be a barrier to riding transit for older
adults (over age 60) who may walk more slowly than others or experience declining physical mobility. This article examines
the relationship between transit ridership and proximity to fixed-route transit stations using survey data for older adults
in Buffalo and Erie County, New York. Demographic and socio-economic characteristics—including age, sex, race, income, possessing
a driver’s license, frequency of leaving home, and personal mobility limitations—are tested but do not display, in bi-variate
analysis, statistically significant differences for transit riders versus non-transit riders. However, features of the built
environment—including distance (actual and perceived) between home and transit stop, transit service level, population density,
number of street intersections, metropolitan location, and neighborhood crime (property and violent) rate—display statistically
significant differences for transit riders versus non-transit riders. Both objective and perceived walking distances to access fixed-route transit show statistically significant differences between transit riders and non-transit
riders. Average walking distance from home to transit for non-transit riders—who mostly live in suburbs—is three times greater
than average walking distance between home and the nearest transit stop for transit riders—who mostly live in the central
city. When asked how near a bus stop is to their homes, transit riders slightly overestimate the actual distance, while non-transit
riders underestimate the distance. 相似文献
2.
Bacterial pore-forming toxins (PFTs) are essential virulence factors of many human pathogens. Knowl- edge of their structure within the membrane is critical for an understanding of their function in pathogenesis and for the development of useful therapy. Atomic force microscopy (AFM) has often been employed to structurally interrogate many membrane proteins, including PFTs, owing to its ability to produce sub-nanometer resolution images of samples under aqueous solution. However, an absolute prerequisite for AFM studies is that the samples are single-layered and closely-packed, which is frequently challenging with PFTs. Here, using the prototypical member of the cholesterol-dependent cytolysin family of PFTs, perfringolysin O (PFO), as a test sample, we have developed a simple, highly robust method that routinely produces clean, closely-packed samples across the entire specimen surface. In this approach, we first use a small Teflon well to prepare the supported lipid bilayer, remove the sample from the well, and then directly apply the proteins to the bilayer. For reasons that are not clear, bilayer preparation in the Teflon well is essential. We anticipate that this simple method will prove widely useful for the preparation of similar samples, and thereby enable AFM imaging of the greatest range of bacterial PFTs to the highest possible resolution. 相似文献
3.
Daniel J. Mayor Thomas R. Anderson David W. Pond Xabier Irigoien 《Journal of Marine Systems》2009,78(4):505
We present concurrent data on ingestion, egg production and the loss of maternal biomass in pre-spring bloom female Calanus finmarchicus incubated under conditions representative of those in situ in the North Atlantic. A balanced metabolic budget was constructed and used to examine the relative importance of ingestion and biomass for fuelling egg production during the incubations. Ingested carbon was not sufficient to meet the observed demands for egg production. More than 80% of the carbon utilised by the females was instead derived from their biomass. Fatty acid analysis demonstrated that the storage reserves, 20:1 (n−9) and 22:1 (n−11), were virtually absent before experimentation began, and therefore could not have been used to supply the carbon required for egg production during the incubations. The C:N mass-specific ratio of the biomass utilised was 4.1, suggesting that the females had instead catabolised protein in order to meet their metabolic demands. These results suggest that C. finmarchicus adopts a sacrificial reproductive strategy when food availability is low. 相似文献
4.
Daniel Puente-Rodríguez 《Coastal management》2013,41(5):426-446
Participation (e.g., stakeholder involvement) has become a central concept in the practice of environmental and coastal zone management. Research has shown that the integration of participation in coastal zone management has positive ecological and social outcomes. In the literature, however, participation is often reported in an unstructured and uncritical manner. Therefore, to find out whether and how there is a useful way to structure and characterize the way the coastal zone management literature deals with participation, we have conducted a literature review. The review was conducted and the literature structured through three central dimensions of participation, namely: power, knowledge, and (visions of) nature. The article concludes that this structured approach to participation enables us to study more systematically the role of participation and might facilitate the governance and learning processes of coastal networks. 相似文献
5.
Edmondo Di Pasquale Daniel Coutellier 《International Journal of Automotive Technology》2017,18(4):631-642
Head on bonnet impact is becoming more and more important in automotive design as regulations on pedestrian safety become more demanding. Despite the relatively low amount of energy involved, these impacts are truly dynamic phenomena as the event duration is comparable with the traveling time of the different wavefronts generated by the impact. In this paper, we show that we can build up a simplified model for the impact based on wave propagation analysis. Using this model, we can analyze head acceleration on existing bonnets or predict it on new ones. Head acceleration in a bonnet impact can thus be estimated over the whole area of the bonnet with a few minutes of CPU. 相似文献
6.
Marine protected areas (MPAs) are now generally accepted as important tools in the protection of coastal biodiversity. It is also likely that they play a positive role in enhancing fisheries. Yet currently, less than 1% of the global oceans are protected, although international agreements have targets ranging from 10–30% coverage. Despite its minuscule size, we consider the current MPA “network” to be beneficial to fisheries, and its running or maintenance cost, therefore, to be a positive contribution to the sustainability of fisheries, or a “beneficial” subsidy (“harmful” subsidies enhance fishing capacity and effort). A method was derived from data in Balmford et al. (PNAS, 101: 9694–9697) to estimate the annual cost of maintaining MPAs as a function of their size, and of the degree of development of the country in question. We provide national costs of the 53 countries that jointly contribute 95% of global fisheries catch and, assuming that this type of subsidy, in a given country, cannot exceed 15% of the ex-vessel value of its fisheries catches, estimated a global MPA subsidy to fisheries of 870 million US$. Given that total subsidies to fisheries currently range from 30–34 billion US$ annually (without MPA costs), this amounts to only 2.5–2.8% of total subsidies to fisheries being devoted explicitly to the maintenance of the biodiversity that sustains them. 相似文献
7.
Silicon dynamics in the Oder estuary, Baltic Sea 总被引:1,自引:0,他引:1
Marianna Pastuszak Daniel J. Conley Christoph Humborg Zbigniew Witek Stanisaw Sitek 《Journal of Marine Systems》2008,73(3-4):250
Studies on dissolved silicate (DSi) and biogenic silica (BSi) dynamics were carried out in the Oder estuary, Baltic Sea in 2000–2005. The Oder estuary proved to be an important component of the Oder River–Baltic Sea continuum where very intensive seasonal DSi uptake during spring and autumn, but also BSi regeneration during summer take place. Owing to the regeneration process annual DSi patterns in the river and the estuary distinctly differed; the annual patterns of DSi in the estuary showed two maxima and two minima in contrast to one maximum- and one minimum-pattern in the Oder River. DSi concentrations in the river and in the estuary were highest in winter (200–250 μmol dm− 3) and lowest (often less than 1 μmol dm− 3) in spring, concomitant with diatom growth; such low values are known to be limiting for new diatom growth. Secondary DSi summer peaks at the estuary exit exceeded 100 μmol dm− 3, and these maxima were followed by autumn minima coinciding with the autumn diatom bloom. Seasonal peaks in BSi concentrations (ca. 100 μmol dm− 3) occurred during the spring diatom bloom in the Oder River. Mass balance calculations of DSi and BSi showed that DSi + BSi import to the estuary over a two year period was 103.2 kt and that can be compared with the DSi export of 98.5 kt. The difference between these numbers gives room for ca. 2.5 kt BSi to be annually exported to the Baltic Sea. Sediment cores studies point to BSi annual accumulation on the level of 2.5 kt BSi. BSi import to the estuary is on the level of ca. 10.5 kt, thus ca. 5 kt of BSi is annually converted into the DSi, increasing the pool of DSi that leaves the system. BSi concentrations being ca. 2 times higher at the estuary entrance than at its exit remain in a good agreement with the DSi and BSi budgeting presented in the paper. 相似文献
8.
Kailin Kroetz Daniel K. Lew James N. Sanchirico Pierce Donovan 《Coastal management》2019,47(2):207-226
The Pacific Halibut Catch Sharing Plan formalized the process for allocating halibut between the Alaska commercial and recreational charter sectors. It included a new program intended to allow for “flexibility” through inter-sectoral trading, permitting charter operators to lease commercial halibut pounds to relax client harvest restrictions. Here we evaluate the first two years of lease market activity and participation. Participation from some commercial quota holders in the lease market suggests that the program provided beneficial flexibility; in fact, the number of transfers to the charter sector was greater than transfers within the commercial sector for some quota types. We also identified a high proportion of self-leasers. However, transfers to the charter sector were on average smaller than within-sector commercial transfers, and total poundage leased by the charter sector was low compared with commercial transfers. Usage of leased quota by the recreational charter sector enables the harvest of larger fish or additional fish, and provides flexibility in catch composition on halibut closure days. Finally, the value-per-pound may be higher in the charter sector, as commercial-to-charter transfer prices approached the commercial ex-vessel price. 相似文献
9.
Brian Gaylord Daniel C. Reed Libe Washburn Peter T. Raimondi 《Journal of Marine Systems》2004,49(1-4):19
The physical–biological linkages controlling the dispersal of spores produced by macroalgae that reside in kelp forests are complicated and laced with feedbacks. Here we discuss the fundamental elements of these interactions. Biological considerations include spore swimming and sinking speeds, their periods of viability in the plankton, and the height of spore release above the seafloor, which together determine the durations over which spores can be swept by horizontal currents before they contact the seafloor. Morphologies and material properties of canopy forming kelps may also influence the drag exerted on passing waters by the kelps, the plants' ability to persist in the face of rapid flows, and thereby the degree to which impinging currents are redirected around, or slowed within, kelp forests. Macroalgal life histories, and the size of spore sources as controlled by the dimensions of kelp forests and the density and fecundity of individuals within them, influence effective dispersal distances as well. Physical considerations encompass the mean speed, direction, and timescales of variability of currents relative to spore suspension times, the interaction of surface gravity waves with currents in producing turbulence in the benthic boundary layer, wind-driven surface mixing, water stratification, and shoreline bathymetry and substratum roughness, all of which can affect the interplay of vertical and horizontal transport of macroalgal spores. Intricate within-forest processes may induce attenuation of current speeds and consequent reductions in seabed shear, along with simultaneous production of small-scale turbulence in kelp wakes. Slower mean currents and smaller eddy scales in turn may attenuate vertical mixing within forests, thus extending spore suspension times. Further complexities likely arise due to changes in the relative rates of horizontal and vertical dispersion, modifications to the overall profiles of vertical mixing, and the creation of fine-scale secondary flows around kelp individuals and substratum features. Under conditions of more rapid currents, submergence of the surface canopy and the establishment of skimming flows at the canopy–fluid interface may introduce additional coherent flow structures that alter rates of fluid exchange to and from the forest. Many of these coupled physical–biological processes are just beginning to be examined in a rigorous fashion in kelp forests, but their potential importance is clear. 相似文献
10.
High resolution structural studies of DNA and DNA binding proteins by atomic force microscopy (AFM) require well-bound samples on suitably flat substrates. Adsorbing the DNA onto a positively charged supported lipid bilayer has previously been shown to be a potentially effective strategy for structural studies with AFM. Here, using our home-built frequency-modulation AFM (FM-AFM), we show that these bilayer substrates are only maximally effective for high resolution AFM when the samples are short, linear DNA, compared with circular plasmid DNA. We find that, with the former sample, the measured width of the DNA is about 2 nm, the known DNA diameter, and there is a clear height modulation along the length of the DNA with a periodicity of about 3.4 nm, in excellent agreement with the known pitch of the double helix. This sample preparation strategy is expected to enable higher resolution studies of DNA and DNA binding proteins with FM-AFM than that can presently be achieved. 相似文献