Apoptin原核表达载体的构建及在大肠杆菌中的表达

目的构建Apoptin的原核表达载体,并制备抗原物质Apoptin融合蛋白。方法在获得Apoptin融合基因的基础上,成功构建了Apoptin的高效原核表达载体pET-28a( )-Apoptin,将该质粒转化至大肠杆菌E.coliBL21(DE3)受体菌中,以IPTG对其进行诱导表达,聚丙烯酰胺凝胶电泳分析目的蛋白。结果转化有Apoptin的原核表达载体pET-28a( )-Apoptin的大肠杆菌E.coliBL21(DE3)经IPTG诱导后,经SDS-PAGE分析,在相对分子质量约17 000的位置出现目的蛋白条带,大小与Apoptin融合蛋白一致。结论Apoptin原核表达载体pET-28a( )-Apoptin能够表达出Apoptin融合蛋白,为进一步的Apoptin研究和制备Apoptin抗体奠定了基础。     

基于"3S"的大型水上桥梁识别系统

目标识别长期以来一直是图像处理研究的热点之一,在军事和民事方面有重要的应用.文中基于大型桥梁的先验知识,研究了通过水域的纹理特征来检出水域的方法,再应用"3S集成技术",通过数字高程模型(DEM)检出桥梁域,从而提供了对大型水上桥梁识别的一种新方法.     

软岩填筑路基的压实特性研究

以武昌至广州客运专线上遇到的白垩系—下第三系以及元古界全风化及强风化千枚状板岩为例,在分析软岩的基本特征的基础上,考虑软岩粗细颗粒相对含量不同对软岩作为路基填料的工程性质的影响,设计正交试验,对不同粗粒含量的软岩试样进行了室内标准重型击实试验,得到了千枚状板岩填筑路基的最优含水量、最大干密度。分析了粗粒含量、击实功的大小以及软岩颗粒的易破碎特性等因素对最大干密度和最优含水量的影响,为软岩填筑路基的施工参数的确定提供了一定的理论依据。     

Quantifying travel time variability at a single bottleneck based on stochastic capacity and demand distributions

Travel time reliability, an essential factor in traveler route and departure time decisions, serves as an important quality of service measure for dynamic transportation systems. This article investigates a fundamental problem of quantifying travel time variability from its root sources: stochastic capacity and demand variations that follow commonly used log-normal distributions. A volume-to-capacity ratio-based travel time function and a point queue model are used to demonstrate how day-to-day travel time variability can be explained from the underlying demand and capacity variations. One important finding is that closed-form solutions can be derived to formulate travel time variations as a function of random demand/capacity distributions, but there are certain cases in which a closed-form expression does not exist and numerical approximation methods are required. This article also uses probabilistic capacity reduction information to estimate time-dependent travel time variability distributions under conditions of non-recurring traffic congestion. The proposed models provide theoretically rigorous and practically useful tools for understanding the causes of travel time unreliability and evaluating the system-wide benefit of reducing demand and capacity variability.     

基于MATLAB的数字基带传输系统的仿真

以数字基带传输系统为例，在数字基带通信系统数学模型分析基础上，设计了总体仿真框图．利用MATLAB对各个子系统进行了建模和封装，建立了数字基带通信系统仿真模型，并对仿真模型进行了测试。结果表明，仿真结果与理论值基本一致。     

交互式识别在SAR图像目标识别中的应用

由于SAR成像机理、强斑点噪声等特点及受分辨率影响，导致SAR图像不直观，特别是对复杂形体小目标的解译相当困难．基于此，文中提供了基于“3S”技术的交互式识别，该方法利用构像方程原理，在“3S”技术（GPS，RS，GIS）和图像数据库的支持下，借助直观的光学图像来识别SAR图上的小目标，以及一些难以判读的大型目标，取得了比较好的识别效果．     

大功率交流试验台新方案探讨

概述了现有大功率交流传动试验试验台的优缺点，提出用交流反馈与交流拖动之间的互馈原理，建立大功率交流试验台的新方案。     

功能分布式双微机系统用于船舶自动导航

本文概述一个专用双微计算机功能分布系统在船舶导航自动化中的应用。分布式计算机系统由于具有模块化、功能强、性能好等特点,能完成较大、较复杂的任务。一个TRS-80微型机和Z-80单板机组成的功能分布式系统,既能完成实时处理、控制等功能,又能完成大量运算。对于特定的目的,可以利用分布系统各单机已有的软件,降低系统设计成本和研究周期。 着重介绍本系统软件主要解决的几个问题:(1)系统的通信;(2)BASIC语言程序的中断处理;(3)在Z-80机中处理中断的方法。     

A multiobjective land development optimization model: the case of New Castle County,Delaware

This paper develops a multiobjective optimization model to consider transportation impacts of the future development of land. The output of the model is the best location and type of land use that has minimal negative transportation effects and uses the maximum available public transportation infrastructure. It provides tools for both planners and transportation engineers and enables them to consider different scenarios of possible policies and land development. Since multiple objectives and their nonlinear structures are considered, the model is solved using mixed integer nonlinear programming. The final results are shown in both tabular and graphical format. The effectiveness of the model is applied to the northern part of New Castle County, Delaware. The results show that the model successfully finds the best locations for both residential and commercial land uses in order to meet several criteria discussed in the paper.