Walking to the bus: perceived versus actual walking distance to bus stops for older adults

The walking trip from an origin or destination to a bus stop or transit station can be a barrier to riding transit for older adults (over age 60) who may walk more slowly than others or experience declining physical mobility. This article examines the relationship between transit ridership and proximity to fixed-route transit stations using survey data for older adults in Buffalo and Erie County, New York. Demographic and socio-economic characteristics—including age, sex, race, income, possessing a driver’s license, frequency of leaving home, and personal mobility limitations—are tested but do not display, in bi-variate analysis, statistically significant differences for transit riders versus non-transit riders. However, features of the built environment—including distance (actual and perceived) between home and transit stop, transit service level, population density, number of street intersections, metropolitan location, and neighborhood crime (property and violent) rate—display statistically significant differences for transit riders versus non-transit riders. Both objective and perceived walking distances to access fixed-route transit show statistically significant differences between transit riders and non-transit riders. Average walking distance from home to transit for non-transit riders—who mostly live in suburbs—is three times greater than average walking distance between home and the nearest transit stop for transit riders—who mostly live in the central city. When asked how near a bus stop is to their homes, transit riders slightly overestimate the actual distance, while non-transit riders underestimate the distance.     

Novel experimental strategy for high resolution AFM imaging of membrane-associated bacterial toxins

Bacterial pore-forming toxins （PFTs） are essential virulence factors of many human pathogens. Knowl- edge of their structure within the membrane is critical for an understanding of their function in pathogenesis and for the development of useful therapy. Atomic force microscopy （AFM） has often been employed to structurally interrogate many membrane proteins, including PFTs, owing to its ability to produce sub-nanometer resolution images of samples under aqueous solution. However, an absolute prerequisite for AFM studies is that the samples are single-layered and closely-packed, which is frequently challenging with PFTs. Here, using the prototypical member of the cholesterol-dependent cytolysin family of PFTs, perfringolysin O （PFO）, as a test sample, we have developed a simple, highly robust method that routinely produces clean, closely-packed samples across the entire specimen surface. In this approach, we first use a small Teflon well to prepare the supported lipid bilayer, remove the sample from the well, and then directly apply the proteins to the bilayer. For reasons that are not clear, bilayer preparation in the Teflon well is essential. We anticipate that this simple method will prove widely useful for the preparation of similar samples, and thereby enable AFM imaging of the greatest range of bacterial PFTs to the highest possible resolution.     

Egg production and associated losses of carbon, nitrogen and fatty acids from maternal biomass in Calanus finmarchicus before the spring bloom

We present concurrent data on ingestion, egg production and the loss of maternal biomass in pre-spring bloom female Calanus finmarchicus incubated under conditions representative of those in situ in the North Atlantic. A balanced metabolic budget was constructed and used to examine the relative importance of ingestion and biomass for fuelling egg production during the incubations. Ingested carbon was not sufficient to meet the observed demands for egg production. More than 80% of the carbon utilised by the females was instead derived from their biomass. Fatty acid analysis demonstrated that the storage reserves, 20:1 (n−9) and 22:1 (n−11), were virtually absent before experimentation began, and therefore could not have been used to supply the carbon required for egg production during the incubations. The C:N mass-specific ratio of the biomass utilised was 4.1, suggesting that the females had instead catabolised protein in order to meet their metabolic demands. These results suggest that C. finmarchicus adopts a sacrificial reproductive strategy when food availability is low.     

The Methodologies of Empowerment? A Systematic Review of the Deployment of Participation in the Coastal Zone Management Literature

Participation (e.g., stakeholder involvement) has become a central concept in the practice of environmental and coastal zone management. Research has shown that the integration of participation in coastal zone management has positive ecological and social outcomes. In the literature, however, participation is often reported in an unstructured and uncritical manner. Therefore, to find out whether and how there is a useful way to structure and characterize the way the coastal zone management literature deals with participation, we have conducted a literature review. The review was conducted and the literature structured through three central dimensions of participation, namely: power, knowledge, and (visions of) nature. The article concludes that this structured approach to participation enables us to study more systematically the role of participation and might facilitate the governance and learning processes of coastal networks.     

Wave propagation in head on bonnet impact: Material and design issues

Head on bonnet impact is becoming more and more important in automotive design as regulations on pedestrian safety become more demanding. Despite the relatively low amount of energy involved, these impacts are truly dynamic phenomena as the event duration is comparable with the traveling time of the different wavefronts generated by the impact. In this paper, we show that we can build up a simplified model for the impact based on wave propagation analysis. Using this model, we can analyze head acceleration on existing bonnets or predict it on new ones. Head acceleration in a bonnet impact can thus be estimated over the whole area of the bonnet with a few minutes of CPU.     

Fine Mapping of a Deafness Mutation hml on Mouse Chromosome 10

Objective To map a mouse deafness gene.identify the underlying mutation and develop a mouse model for human deafncss.Methods Genetic lindage cross and genome scan wer used to map a novel mutation named hypoplasia of the membranous labyrinth (hml),which causes hearing loss in mutant mice.Results ① hml was mapped on mouse Chr 10 (～43 cM from the centromere) suggests that the homologous human gene is on 12q22--q24, which was defined on the basis of known mouse-human homologies (OMIM,2004). ② This study has generated 25 polymorphic microsateUite markers, placed 3 known human genes in the correct order in a high-resolution mouse map and narrowed the hml candidate gene region to a 500 kb area.     

Marine Protected Area Costs as “Beneficial” Fisheries Subsidies: A Global Evaluation

Marine protected areas (MPAs) are now generally accepted as important tools in the protection of coastal biodiversity. It is also likely that they play a positive role in enhancing fisheries. Yet currently, less than 1% of the global oceans are protected, although international agreements have targets ranging from 10–30% coverage. Despite its minuscule size, we consider the current MPA “network” to be beneficial to fisheries, and its running or maintenance cost, therefore, to be a positive contribution to the sustainability of fisheries, or a “beneficial” subsidy (“harmful” subsidies enhance fishing capacity and effort). A method was derived from data in Balmford et al. (PNAS, 101: 9694–9697) to estimate the annual cost of maintaining MPAs as a function of their size, and of the degree of development of the country in question. We provide national costs of the 53 countries that jointly contribute 95% of global fisheries catch and, assuming that this type of subsidy, in a given country, cannot exceed 15% of the ex-vessel value of its fisheries catches, estimated a global MPA subsidy to fisheries of 870 million US$. Given that total subsidies to fisheries currently range from 30–34 billion US$ annually (without MPA costs), this amounts to only 2.5–2.8% of total subsidies to fisheries being devoted explicitly to the maintenance of the biodiversity that sustains them.     

Silicon dynamics in the Oder estuary, Baltic Sea

Studies on dissolved silicate (DSi) and biogenic silica (BSi) dynamics were carried out in the Oder estuary, Baltic Sea in 2000–2005. The Oder estuary proved to be an important component of the Oder River–Baltic Sea continuum where very intensive seasonal DSi uptake during spring and autumn, but also BSi regeneration during summer take place. Owing to the regeneration process annual DSi patterns in the river and the estuary distinctly differed; the annual patterns of DSi in the estuary showed two maxima and two minima in contrast to one maximum- and one minimum-pattern in the Oder River. DSi concentrations in the river and in the estuary were highest in winter (200–250 μmol dm^− 3) and lowest (often less than 1 μmol dm^− 3) in spring, concomitant with diatom growth; such low values are known to be limiting for new diatom growth. Secondary DSi summer peaks at the estuary exit exceeded 100 μmol dm^− 3, and these maxima were followed by autumn minima coinciding with the autumn diatom bloom. Seasonal peaks in BSi concentrations (ca. 100 μmol dm^− 3) occurred during the spring diatom bloom in the Oder River. Mass balance calculations of DSi and BSi showed that DSi + BSi import to the estuary over a two year period was 103.2 kt and that can be compared with the DSi export of 98.5 kt. The difference between these numbers gives room for ca. 2.5 kt BSi to be annually exported to the Baltic Sea. Sediment cores studies point to BSi annual accumulation on the level of 2.5 kt BSi. BSi import to the estuary is on the level of ca. 10.5 kt, thus ca. 5 kt of BSi is annually converted into the DSi, increasing the pool of DSi that leaves the system. BSi concentrations being ca. 2 times higher at the estuary entrance than at its exit remain in a good agreement with the DSi and BSi budgeting presented in the paper.     

Circumventing the weight-versus-footprint tradeoffs in vehicle fuel economy regulation

China, Japan, and the European Union use weight-based fuel economy standards, whereas the US Department of Transportation favors footprint-based standards. In this paper we offer a way of reconciling these approaches. Weight-based standards tend to focus regulatory incentives on technology rather than downsizing, but they provide no incentive for weight reduction. Footprint-based standards, by contrast, motivate vehicle manufacturers to reduce weight without reducing footprint, but only to the extent that they are also motivated to increase footprint without increasing weight. Neither approach discriminates between beneficial and detrimental weight-changing strategies. However, the tradeoffs between weight and footprint can be circumvented by employing a weight-based standard, which does not create weight-changing incentives, in combination with complementary regulatory measures that would be focused specifically and exclusively on motivating beneficial weight reduction strategies.