Novel experimental strategy for high resolution AFM imaging of membrane-associated bacterial toxins

Bacterial pore-forming toxins （PFTs） are essential virulence factors of many human pathogens. Knowl- edge of their structure within the membrane is critical for an understanding of their function in pathogenesis and for the development of useful therapy. Atomic force microscopy （AFM） has often been employed to structurally interrogate many membrane proteins, including PFTs, owing to its ability to produce sub-nanometer resolution images of samples under aqueous solution. However, an absolute prerequisite for AFM studies is that the samples are single-layered and closely-packed, which is frequently challenging with PFTs. Here, using the prototypical member of the cholesterol-dependent cytolysin family of PFTs, perfringolysin O （PFO）, as a test sample, we have developed a simple, highly robust method that routinely produces clean, closely-packed samples across the entire specimen surface. In this approach, we first use a small Teflon well to prepare the supported lipid bilayer, remove the sample from the well, and then directly apply the proteins to the bilayer. For reasons that are not clear, bilayer preparation in the Teflon well is essential. We anticipate that this simple method will prove widely useful for the preparation of similar samples, and thereby enable AFM imaging of the greatest range of bacterial PFTs to the highest possible resolution.     

Egg production and associated losses of carbon, nitrogen and fatty acids from maternal biomass in Calanus finmarchicus before the spring bloom

We present concurrent data on ingestion, egg production and the loss of maternal biomass in pre-spring bloom female Calanus finmarchicus incubated under conditions representative of those in situ in the North Atlantic. A balanced metabolic budget was constructed and used to examine the relative importance of ingestion and biomass for fuelling egg production during the incubations. Ingested carbon was not sufficient to meet the observed demands for egg production. More than 80% of the carbon utilised by the females was instead derived from their biomass. Fatty acid analysis demonstrated that the storage reserves, 20:1 (n−9) and 22:1 (n−11), were virtually absent before experimentation began, and therefore could not have been used to supply the carbon required for egg production during the incubations. The C:N mass-specific ratio of the biomass utilised was 4.1, suggesting that the females had instead catabolised protein in order to meet their metabolic demands. These results suggest that C. finmarchicus adopts a sacrificial reproductive strategy when food availability is low.     

Safety of child passengers who ride to school on a motorcycle: An observational study in two Argentine cities

In low- and middle-income countries, the motorcycle has increasingly become a means of family transportation due to its low cost and the poor quality of public transportation. Consequently, many child motorcycle passengers are at an elevated risk of serious injury in the event of a traffic crash. The available research on child safety devices is scarce and focused mainly on helmet use, while other devices (e.g. high-visibility clothing) have received little attention. This observational study documented the safety conditions and related factors of primary schoolchildren who rode to school as motorcycle passengers in two cities of Argentina.Data was obtained in the vicinity of 27 public primary schools in two cities of Argentina (one mid-sized city and the other a small city by the country's standards) between May 2018 and September 2019. A total of 1440 motorcycle occupants (656 drivers and 784 child passengers) were observed.The use of an adequate helmet was lower among children (vs. drivers). Most of the children were unable to reach the motorcycle's footrests, did not use high-visibility clothing or a fastening device, carried a backpack, and mounted and dismounted the motorcycle in an unsafe place. Some children rode in front of the driver or in a motorcycle with three or four occupants. Use of helmet by drivers and driver sex (woman) were related with an increase in safety in children. The indicators were worse in the larger city.The results revealed a low level of adoption of safety measures in children as well as in adults in both cities. This suggests that motorcycle travel is unsafe. Greater effort is needed to improve motorcycle safety, especially for children. It is important to debate regulatory details such as a minimum age for passengers, a limit on the number of passengers, and other safety criteria. It is also important to conduct educational campaigns that include the distribution of safety devices.     

The Gini index of demand imbalances in public transport

Transportation - The paper studies a general bidirectional public transport line along which demand varies by line section. The length of line sections also varies, and therefore their contribution...     

Trip generation of vulnerable populations in three Canadian cities: a spatial ordered probit approach

This paper provides an analysis of trip generation of three vulnerable groups: single-parent families, low income households, and the elderly. It compares the mobility of these groups to that of the general population in three Canadian urban areas of Hamilton, Montreal and Toronto, based on data from large-sample metropolitan transport surveys. An ordered probit model with spatially expanded coefficients is used for the analysis. Spatial expansion shows that there are spatial mobility trends for elderly populations and low-income populations even after socio-economic attributes are accounted for. Such spatial differences are not generally found for single parent families. This novel spatial analysis provides clues as to where vulnerable populations may experience greater degrees of social exclusion. It provides information to help prioritize transportation infrastructure projects or other social programs to take into account the needs of vulnerable populations with the lowest levels of mobility.     

Environmental and transport effects of warehouse relocationing: evidence from Norway

Reducing traffic volumes and CO2-emissions from freight transport has proven difficult in many countries. Although the increasing suburbanization of warehouses is seen as a relevant land use trend, comprehensive analyses of their impact remain scarce. This study uses real data in modeling transport, costs, environmental and modal effects from warehouse relocations around Oslo and Trondheim (Norway). Results indicate that for Oslo, traffic performance (ton-km), CO2-emissions, and transport costs increase following warehouse suburbanization. For Trondheim, transport performance and CO2-emissions increase less, while transport costs decrease marginally. We conclude that specific case characteristics (geography and trade patterns) are important in determining the strength and direction of effects, and expect that common concomitant developments (warehouse centralization and consolidation) would lead to more pronounced results. Our findings confirm some, but challenge other, findings from the relatively scarcely literature available. Finally, the study's more general insights and observations can help advance similar analyses beyond Norway.     

Air transport and economic growth: a review of the impact mechanism and causal relationships

ABSTRACT

The impacts of air transport on the economy arise both directly, via activity in the aviation sector; and indirectly, via increased spending and wider economic benefits associated with improved access to resources, markets, technology and economic mass. Economic activity, in turn, supports and generates demand for air transport. Despite its potential importance, the reciprocal nature of the causal relationship between air transport and economic performance has remained somewhat understudied. This paper provides a synthesis review of the channels the aviation sector interacts with regional economy. The review focuses on quantitative studies that contribute to the state-of-the-art understandings of the causality. We find that the reciprocal causal relationship is more likely to prevail in less developed economies. For more developed economies, only one direction of the causality is recognised, which runs from air transport to economic growth. Especially substantial is the effect of airline enplanement on service-related employment. The reverse direction of the relationship is, however, not as significant as believed in a causal sense within the developed world. Therefore, cautions need to be taken when applying income elasticities (such as the elasticity of air passenger demand with respect to GDP) in air travel demand forecasting, which implicitly assumes that economic growth causally leads to air traffic increment. Based on the fundamental links between air transport and economic growth, some typical imperfections and inefficiencies in aviation markets are discussed and promising avenues for future research are proposed.     

Does transport help people to gain employment? A systematic review and meta-analysis of the empirical evidence

ABSTRACT

The role of transport in providing access to employment has received considerable attention. Since transport policies may be motivated by assumed effects on employment probability outcomes, it is important to establish the nature of the relationship between transport and employment outcomes. While the majority of the empirical evidence suggests a positive association, it is not conclusive or consistent and often shows mixed results. To address this confusion, our study has systematically reviewed this evidence base and synthesised it through meta-analysis. We first identified 93 studies that quantitatively assessed the impact of transport on employment outcomes. By systematically merging the empirical evidence, this study establishes a positive association between transport and employment outcomes, with varying effects for four identified categories of transport measures (or combinations thereof): car ownership, public transport access, commute times, and job accessibility levels. This positive association persists in studies that control for endogeneity between transport and employment, but a larger evidence base is needed to establish a more robust relationship, in particular for cities and smaller (rural) areas outside the US-context and with regard to public transport. We then selected 20 methodologically comparable studies for inclusion in the meta-analysis. Our meta-regression models clearly demonstrate that car ownership significantly increases individual employment probabilities, in particular among welfare recipients. Young drivers benefit from access to household cars when these are not in use by their parents, and they are more sensitive to the time and cost implications of longer commutes. While our systematic review suggests that better access to public transport and higher levels of job accessibility increases employment probabilities, meta-regression analysis requires more consistent transport measures. The findings in this study are important for policymakers in that they imply that job seekers may benefit from public policies targeted at improving their access to public transport, in particular for people without access to cars and in areas with fewer job opportunities.     

Specification,transferability and the effect of data outliers in modeling the choice of mode in urban travel

Three problems of great importance to urban travel demand modeling using multinominal logit models are examined in this paper. They are (1) the effect of data outliers on model coefficients; (2) the effect of model specification on coefficients and model explanatory power; and (3) the transferability of model coefficients within the region, between regions, and in time.Four data sets are used in the study. They are: Washington, D.C., Minneapolis-St. Paul, and two data sets from the San Francisco Bay Area, Pre-BART and Post-BART. The data are standard home-interview survey data appended with network supplied modal travel cost and time information.The findings of the research are occasionally contradictory; the majority of the evidence supports the following conclusions. The outliers do not have a statistically significant effect (at 0.05 level) on the coefficients; however, the outliers can have a substantial effect on the point estimates of some of the coefficients. Model specification has an impact on model coefficients and model explanatory power. In particular, the definition of out-of-vehicle travel time appears to be important and, if available, the use of separate walk and wait times is preferred over their sum, the out-of-vehicle time. Finally, the model coefficients do not appear transferable within region, between regions, or in time.Research was supported in part by the Alfred P. Sloan Foundation, through grant 74-12-8 to the Department of Economics, University of California, Berkeley and by the National Science Foundation, through grant APR 74-20392, Research Applied to National Needs Program, to the University of California, Berkeley.