SIMPLE METHOD OF PUNCHING AND RE-LOCATING TISSUES FOR MANUAL CONSTRUCTION OF TISSUE MICROARRAY

A series of human tissue samples and cultured cell lines were formalin-fixed and paraffin-embedded. Specimen cylinder ( 1.2 - 1.8mm) were punched by a modified bone marrow biopsy needle and arrayed on a recipient paraffin block. Microscopic analysis on the sections from this tissue microarray (TMA) block demonstrated that the spots of tissues and cells were well preserved, and the cultured cell samples were successfully embedded from 5 × 104 to 2 × 105 in number. These TMA sections were also suitable for immunohistochemistry and RNA in situ hybridization.     

Shared taxis: modelling the choice of a paratransit mode in Santiago de Chile

The shared taxi is a special public transport mode, typical of Chilean cities. It operates with cars offering a maximum capacity of four seats, a predefined coverage area and a route that is fixed in principle, but can be adapted to meet passengers’ needs. During a normal day in Santiago, almost 700,000 trips use shared taxis during one of their stages. This represents about 4% of the total trips made in the city, and this modal share increases in zones and periods with low Metro and bus coverage. This study is a first attempt at studying shared taxis as a relevant transport alternative, analysing its main attributes and modelling its demand. With this purpose, after an analysis of the network and its operation, a revealed preference survey (including perceptual indicators) was applied to public transport users in Santiago who had shared taxi as a feasible alternative. Results show a positive evaluation of the mode’s unique attributes, such as the possibility of travelling seated, reducing transfers and alighting at a convenient destination. The subjective valuation of the attributes derived from the models confirm the strong penalty assigned by Chilean users to alternatives implying transfers or increased walking times. The analysis also shows that studying the characteristics of shared taxi users is relevant in a discussion about its regulation and modernization, considering that, while it is desirable to preserve its positive attributes, this should be done in a context of efficient integration with the rest of the transport system.

     

American and European transportation responses to the 1973–74 oil embargo

A survey of U.S. and European responses to the energy crises engendered by the Arab boycott of October 1973–April 1974 is presented. U.S. Government policy actions with respect to allocation, rationing, price, and conservation measures are reviewed and contrasted with those of affected European governments. The results of those policies and their effects on transportation energy supply and demand behavior with respect to both commodity and passenger movement are described.Based on short- and long-term impact assessments of the policies considered, the authors identify significant research requirements.The authors were heavily engaged in U.S. responses to the energy emergency, and were asked to undertake study missions to those European nations significantly affected by the crisis to seek out avenues for mutual aid, and joint research, in resolving common energy problems. This paper is one product of those study missions.     

Step-tolling with price-sensitive demand: Why more steps in the toll make the consumer better off

Most dynamic models of congestion pricing use fully time-variant tolls. However, in practice, tolls are uniform over the day, or at most have just a few steps. Such uniform and step tolls have received surprisingly little attention from the literature. Moreover, most models that do study them assume that demand is insensitive to the price. This seems an empirically questionable assumption that, as this paper finds, strongly affects the implications of step tolling for the consumer. In the bottleneck model, first-best tolling has no effect on the generalised price, and thus consumer surplus remains the same as without tolling. Conversely, under price-sensitive demand, step tolling increases the price, making the consumer worse off. The more steps the toll has, the closer it approximates the first-best toll, thereby increasing the welfare gain and making consumers better off. This indicates the importance for real-world tolls to have as many steps as possible: this not only raises welfare, but may also increase the political acceptability of the scheme by making consumers better off.     

Cycle commuting in Belgium: Spatial determinants and ‘re-cycling’ strategies

This paper attempts to explain the spatial variation of the use of a bicycle for commuting to work at the level of the 589 municipalities in Belgium. Regression techniques were used and special attention was paid to autocorrelation, heterogeneity and multicollinearity. Spatial lag models were used to correct for the presence of spatial dependence and a disaggregated modelling strategy was adopted for the northern and southern parts of the country. The results show that much of the inter-municipality variation in bicycle use is related to environmental aspects such as the relief, traffic volumes and cycling accidents. Town size, distance travelled and demographic aspects also have some effect. In addition, there are regional differences in the effects of the structural covariates on bicycle use: the impact of variables such as traffic volume and cycling accidents differs substantially between the north and the south of the country. This paper also suggests that high rates of bicycle use in one municipality stimulate cycling in neighbouring municipalities, and hence that a mass effect can be initiated, i.e. more cycle commuting encourages even more commuters in the area to cycle. These findings provide some recommendations for decision-makers wishing to promote a shift from car to bicycle use.     

The role of volume-delay functions in forecasting and evaluating congestion charging schemes: the Stockholm case

This paper uses observations from before and during the Stockkholm congestion charging trial in order to validate and improve a transportation model for Stockholm. The model overestimates the impact of the charges on traffic volumes while at the same time it substantially underestimates the impact on travel times. These forecast errors lead to considerable underestimation of economic benefits which are dominated by travel time savings. The source of error lies in the static assignment that is used in the model. Making the volume-delay functions (VDFs) steeper only marginally improves the quality of forecast but strongly impacts the result of benefit calculations. We therefore conclude that the dynamic assignment is crucial for an informed decision on introducing measures aimed at relieving congestion. However, in the absence of such a calibrated dynamic model for a city, we recommend that at least a sensitivity analysis with respect to the slope of VDFs is performed.     

A latent class accelerated hazard model of social activity duration

Over the past decade, activity scheduling processes have gained increasing attention in the field of transportation research. However, still little is known about the scheduling of social activities even though these activities account for a large and growing portion of trips. This paper contributes to this knowledge. We analyze how the duration of social activities is influenced by social activity characteristics and characteristics of the relationship between the respondent and the contacted person(s). To that end, a latent class accelerated hazard model is estimated, based on social interaction diary data that was collected in the Netherlands in 2008. Chi-square tests and analyses of variance are used to test for significant relations between the latent classes and personal and household characteristics. Findings suggest that the social activity characteristics and the characteristics of the relationship between the socializing persons are highly significant in explaining social activity duration. This shows that social activities should not be considered as a homogenous set of activities and it underlines the importance of including the social context in travel-behavior models. Moreover, the results indicate that there is a substantial amount of latent heterogeneity across the population. Four latent classes are identified, showing different social activity durations, and different effects for both categories of explanatory variables. Latent class membership can be explained by household composition, socio-economic status (education, income and work hours), car ownership and the number of interactions in 2 days.     

Estimating the value of risk reductions for car drivers when pedestrians are involved: a case study in Spain

We estimated the benefits associated with reducing fatal and severe injuries from traffic accidents using a stated choice experiment where choice situations were generated through a statistically efficient design. Specifically, the risk variables were defined as the expected annual number of vehicle car-users that suffered their death or were severely injured in a traffic accident. In addition, and differing from previous research, the number of pedestrians that died or were severely injured in traffic accidents per year was also included as a risk attribute in the choice experiment, to attempt at measuring drivers’ willingness to pay to reduce the risk of hitting pedestrians in a crash. The empirical setting was a choice of route for a particular trip that a sample of car drivers periodically undertakes in Tenerife, Spain. Models were estimated accounting for random taste heterogeneity and pseudo-panel data correlation. The median of the distribution of simulated parameters was used to obtain a representative measure for the monetary valuation of risk reductions. We found that the ratio between the values of reducing the risk of suffering a serious injury and that of reducing a fatality was approximately 18 %. Further, and quite novel, we also found that the value of reducing a pedestrian fatality was 39 % of the value of reducing a car occupant fatality.     

Impact of regional population density on walking behavior

Land use can influence walking (measured by the number of steps) and so the health of people. This paper presents the result of empirical research on the impact of regional population densities (inhabitants per inhabitable area) on the number of steps (all steps, both outdoors and indoors). With data collected from almost 11,000 respondents in 148 Japanese regions, we estimate polynomial regression models, the total number of steps being the dependent variable and densities being the main independent variable. Regional population density significantly affects the number of steps after controlling for individual and household attributes. The estimated population density that maximizes the number of steps is around 11,000?persons/km². Increasing densities, up to levels of around 11,000?inhabitants/km², could increase walking and consequently the health of inhabitants. The population density elasticity of the number of steps is 0.046–0.049 in a simple log linear regression model without a peak.