SIMPLE METHOD OF PUNCHING AND RE-LOCATING TISSUES FOR MANUAL CONSTRUCTION OF TISSUE MICROARRAY

A series of human tissue samples and cultured cell lines were formalin-fixed and paraffin-embedded. Specimen cylinder ( 1.2 - 1.8mm) were punched by a modified bone marrow biopsy needle and arrayed on a recipient paraffin block. Microscopic analysis on the sections from this tissue microarray (TMA) block demonstrated that the spots of tissues and cells were well preserved, and the cultured cell samples were successfully embedded from 5 × 104 to 2 × 105 in number. These TMA sections were also suitable for immunohistochemistry and RNA in situ hybridization.     

Shared taxis: modelling the choice of a paratransit mode in Santiago de Chile

The shared taxi is a special public transport mode, typical of Chilean cities. It operates with cars offering a maximum capacity of four seats, a predefined coverage area and a route that is fixed in principle, but can be adapted to meet passengers’ needs. During a normal day in Santiago, almost 700,000 trips use shared taxis during one of their stages. This represents about 4% of the total trips made in the city, and this modal share increases in zones and periods with low Metro and bus coverage. This study is a first attempt at studying shared taxis as a relevant transport alternative, analysing its main attributes and modelling its demand. With this purpose, after an analysis of the network and its operation, a revealed preference survey (including perceptual indicators) was applied to public transport users in Santiago who had shared taxi as a feasible alternative. Results show a positive evaluation of the mode’s unique attributes, such as the possibility of travelling seated, reducing transfers and alighting at a convenient destination. The subjective valuation of the attributes derived from the models confirm the strong penalty assigned by Chilean users to alternatives implying transfers or increased walking times. The analysis also shows that studying the characteristics of shared taxi users is relevant in a discussion about its regulation and modernization, considering that, while it is desirable to preserve its positive attributes, this should be done in a context of efficient integration with the rest of the transport system.

     

American and European transportation responses to the 1973–74 oil embargo

A survey of U.S. and European responses to the energy crises engendered by the Arab boycott of October 1973–April 1974 is presented. U.S. Government policy actions with respect to allocation, rationing, price, and conservation measures are reviewed and contrasted with those of affected European governments. The results of those policies and their effects on transportation energy supply and demand behavior with respect to both commodity and passenger movement are described.Based on short- and long-term impact assessments of the policies considered, the authors identify significant research requirements.The authors were heavily engaged in U.S. responses to the energy emergency, and were asked to undertake study missions to those European nations significantly affected by the crisis to seek out avenues for mutual aid, and joint research, in resolving common energy problems. This paper is one product of those study missions.     

Cycle commuting in Belgium: Spatial determinants and ‘re-cycling’ strategies

This paper attempts to explain the spatial variation of the use of a bicycle for commuting to work at the level of the 589 municipalities in Belgium. Regression techniques were used and special attention was paid to autocorrelation, heterogeneity and multicollinearity. Spatial lag models were used to correct for the presence of spatial dependence and a disaggregated modelling strategy was adopted for the northern and southern parts of the country. The results show that much of the inter-municipality variation in bicycle use is related to environmental aspects such as the relief, traffic volumes and cycling accidents. Town size, distance travelled and demographic aspects also have some effect. In addition, there are regional differences in the effects of the structural covariates on bicycle use: the impact of variables such as traffic volume and cycling accidents differs substantially between the north and the south of the country. This paper also suggests that high rates of bicycle use in one municipality stimulate cycling in neighbouring municipalities, and hence that a mass effect can be initiated, i.e. more cycle commuting encourages even more commuters in the area to cycle. These findings provide some recommendations for decision-makers wishing to promote a shift from car to bicycle use.     

Estimating the value of risk reductions for car drivers when pedestrians are involved: a case study in Spain

We estimated the benefits associated with reducing fatal and severe injuries from traffic accidents using a stated choice experiment where choice situations were generated through a statistically efficient design. Specifically, the risk variables were defined as the expected annual number of vehicle car-users that suffered their death or were severely injured in a traffic accident. In addition, and differing from previous research, the number of pedestrians that died or were severely injured in traffic accidents per year was also included as a risk attribute in the choice experiment, to attempt at measuring drivers’ willingness to pay to reduce the risk of hitting pedestrians in a crash. The empirical setting was a choice of route for a particular trip that a sample of car drivers periodically undertakes in Tenerife, Spain. Models were estimated accounting for random taste heterogeneity and pseudo-panel data correlation. The median of the distribution of simulated parameters was used to obtain a representative measure for the monetary valuation of risk reductions. We found that the ratio between the values of reducing the risk of suffering a serious injury and that of reducing a fatality was approximately 18 %. Further, and quite novel, we also found that the value of reducing a pedestrian fatality was 39 % of the value of reducing a car occupant fatality.     

The development of the TIGRIS XL model: A bottom-up approach to transport, land-use and the economy

The project appraisal method in the Netherlands distinguishes direct effects, indirect distribution effects and indirect generative effects. Transport models are generally available for calculating the benefits of transport projects to travellers as part of the direct effects. The TIGRIS XL model, a Land-Use and Transport Interaction model, adds indirect distribution effects for the housing and labour market, by modelling changes in the spatial distribution of jobs and residents. This paper describes the current TIGRIS XL model and how it can be applied in the evaluation of transport projects. It refers to work done with the TIGRIS XL model to improve the calculation of the direct benefits by applying the so-called ‘logsum’ method. Finally it discusses ideas on how to extend the TIGRIS XL model to address indirect generative effects as well.The current TIGRIS XL model, its integration with the National Model System (the standard tool for the Dutch government to analyse the effects on passenger transport) and basic design principles are the starting point of such an exploration. These design principles include:

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an emphasis on detail, both spatial as well as in socio-economic segments, to account for differences in the availability of choice alternatives and in choice behaviour, and to provide impacts by region and socio-economic group;

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the requirement that the relationships used rest on an empirical foundation.

The post-processing methods proposed on this paper for the calculation of the direct benefits and indirect generative effects are consistent with these design principles. To calculate the indirect generative effects, a post-processing module is proposed which transforms changes in travel times and land-use into agglomeration effects. These agglomeration effects combine several of the generative effects, like scale economies, an increasing variety of products, labour market matches and knowledge spill-over. The effects of changes in the agglomeration indicator on productivity can be estimated with existing data sources, as was demonstrated in the UK (Graham, 2005).     

In situ colonization of marine biofilms on UNS S32760 duplex stainless steel coupons in areas with different water qualities: Implications for corrosion potential behavior

In the presence of biofilms, stainless steels （SS） exhibits an increase in corrosion potential, called ennoblement. In the present study, the corrosion potential （Eco,） behavior of the duplex SS UNS S32760 was recorded simultaneously with the in situ marine biofilm formation in two areas at Arraial do Cabo, Southeastern Brazil. The biofilm at Forno Harbor （an anthropogenically disturbed area） was characterized by higher relative abundances of Bacteria at day 2, followed by diatoms （especially Navicula sp.） on day 10 and dinoflagellates on day 18, whereas no clear trend was recorded at Cabo Frio Island （an undisturbed area）. The ennoblement of Ecor values was site-dependent. In a complementary laboratory assay, biofilms were removed and the Eco values registered in sterile conditions for the subsequent 10 days and corroborated in situ results. Understanding biofilms and SS interactions has important implications for materials science and engineering decisions as well as helping to fill in imnortant gaps in this knowledge.     

Ballast Water Treatment systems: “Old” and “New” ones

This paper offers an overview of the currently available Ballast Water Treatment systems, their efficacy in reducing the number of organisms in relation with the IMO and other, future standards, and explores potentially new and promising technologies and current gaps.