放射诱导p16基因胰腺癌靶向性表达的研究

目的　构建 pcDNA3.1 Egr.1p-p16重组质粒并检测其在人胰腺癌 JF305细胞中的辐射诱导表达。方法　将人p16 cDNA基因连接到有辐射诱导特性的早期反应因子 Egr.1p的下游,构建成 pcDNA3.1 -Egr.1p- p16 重组质粒,利用脂质体介导转染人胰腺癌细胞系 JF305 细胞;采用 RT- PCR方法和 Western blot方法检测不同剂量 X射线照射后,被转染细胞中 p16 的转录和表达水平。结果　酶切鉴定证实 pcDNA3. 1 Egr. 1p -p16 重组质粒构建正确。被pcDNA3.1 Egr.1p -p16重组质粒转染的人胰腺癌 JF305细胞经不同剂量 X射线照射后,p16 基因表达均高于未照射组。结论　X射线可诱导 pcDNA3.1 -Egr.1p -p16重组质粒在人胰腺癌 JF305细胞中表达增强。     

抑癌基因p16、Rb的表达与胆囊癌预后的关系

目的　研究抑癌基因 p1 6及Rb的表达与胆囊癌预后的关系。方法　用SP免疫组化法同步检测 41例胆囊癌患者抑癌基因 p1 6及Rb的蛋白表达。 结果　p1 6与Rb蛋白表达阳性组生存率明显优于阴性组。结论　p1 6及Rb在胆囊癌的预后判定中具有重要价值 ,同步检测两者的预后价值优于单一检测。     

Prospective randomized trial of surgery combined with preoperative and postoperative radiotherapy for rectal carcinoma

Objective To assess the effect of surgery combined with preoperative and postoperative radiotherapy ("sandwich" treatment) in rectal carcinoma. Methods From October 1990 to January 2002, 260 patients with stage Ⅱ (117 patients) and stage Ⅲ (143 patients) rectal carcinoma were randomly divided into three groups: "sandwich" group (92 patients, group A), postoperative radiotherapy group (98 patients, Group B) and operation group (70 patients, Group C). The preoperative accelerated hyperfractionation (15 Gy/6f/3d) was given for "sandwich" group which was followed by conventional postoperative fractionation (DT 35 - 40 Gy/3.5 - 4 weeks). Patients in Group B were given postoperative radiotherapy (Dr 50 Gy/5 weeks). Patients treated with surgery alone served as control. Results The local recurrence rates of Group A, B and C were 5.4% (5/92), 16.3% (16/98) and 64.3% (45/70), respectively (X2 =5.726, P=0.017); and the distant metastasis rates were 6.5% (6/92), 28. 6%(28/98) and 31.4%(22/70), respectively (X2 =15. 703, P= 0. 001). The 3-ycar survival rate was 86. 9%(80/92), 62.2%(61/98) and 51.4%(36/70), respectively (X2 =15. 141, P=0. 001). The 5-year survival rate was 68. 5%(63/92), 54.1%(54/98)and 41.4%(29/70), respectively (X2 =4. 218, P=0.04). The Ⅰ and Ⅱ grades of radiation entero-colitis in Group A and Group B were 7.6% (7/92) and 6.1% (6/98), respectively (X2 = 0. 164, P= 0. 685). Conclusion Surgery combined with preoperative and postoperative radiotherapy can improve the survival rate and reduce the local recurrence rate in rectal carcinoma patients with stage Duke's B (Ⅱ) and C (Ⅲ).