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We measured the abundance and biomass of phototrophic and heterotrophic microbes in the upper mixed layer of the water column in ice-covered Franklin Bay, Beaufort Sea, Canada, from December 2003 to May 2004, and evaluated the influence of light and nutrients on these communities by way of a shipboard enrichment experiment. Bacterial cell concentrations showed no consistent trends throughout the sampling period, averaging (± SD) 2.4 (0.9) × 108 cells L− 1; integrated bacterial biomass for the upper mixed layer ranged from 1.33 mg C m− 3 to 3.60 mg C m− 3. Small cells numerically dominated the heterotrophic protist community in both winter and spring, but in terms of biomass, protists with a diameter > 10 µm generally dominated the standing stocks. Heterotrophic protist biomass integrated over the upper mixed layer ranged from 1.23 mg C m− 3 to 6.56 mg C m− 3. Phytoplankton biomass was low and variable, but persisted during the winter period. The standing stock of pigment-containing protists ranged from a minimum value of 0.38 mg C m− 3 in winter to a maximal value of 6.09 mg C m− 3 in spring and the most abundant taxa were Micromonas-like cells. These picoprasinophytes began to increase under the ice in February and their population size was positively correlated with surface irradiance. Despite the continuing presence of sea ice, phytoplankton biomass rose by more than an order of magnitude in the upper mixed layer by May. The shipboard experiment in April showed that this phototrophic increase in the community was not responsive to pulsed nutrient enrichment, with all treatments showing a strong growth response to improved irradiance conditions. Molecular (DGGE) and microscopic analyses indicated that most components of the eukaryotic community responded positively to the light treatment. These results show the persistence of a phototrophic inoculum throughout winter darkness, and the strong seasonal response by arctic microbial food webs to sub-ice irradiance in early spring.  相似文献   
2.
We examine the role of social networks in enabling access to private-vehicle transportation, through getting rides and borrowing cars. Based on qualitative findings from ten focus group discussions with recent Mexican immigrants to California, half of whom have no car, we describe the extent to which participants depend on rides and borrowed cars for transportation. We highlight the unique aspects of informal access to cars, drawing on social exchange theory and related research to characterize the procurement process and likely levels of exchange. We discuss the implications of these findings for transportation services that might serve this and other community groups.  相似文献   
3.
The large quantities of particles delivered by the Mackenzie River to the coastal Beaufort Sea (Arctic Ocean) have implications for the spatial distribution, composition and productivity of its bacterial communities. Our objectives in this study were: (1) to assess the contribution of particle-associated bacteria (fraction  3 µm) to total bacterial production and their relationships with changing environmental conditions along a surface water transect; (2) to examine how particle-based heterotrophy changes over the annual cycle (Nov 2003–Aug 2004); and (3) to determine whether particle-associated bacterial assemblages differ in composition from the free-living communities (fraction < 3 µm). Our transect results showed that particle-associated bacteria contributed a variable percentage of leucine-based (BP-Leu) and thymidine-based (BP-TdR) bacterial production, with values up to 98% at the inshore, low salinity stations. The relative contribution of particle-associated bacteria to total BP-Leu was positively correlated with temperature and particulate organic material (POM) concentration. The annual dataset showed low activities of particle-associated bacteria during late fall and most of the winter, and a period of high particle-associated activity in spring and summer, likely related to the seasonal inputs of riverine POM. Results from catalyzed reporter deposition for fluorescence in situ hybridization (CARD-FISH) confirmed the dominance of Bacteria and presence of Archaea (43–84% and 0.2–5.5% of DAPI counts, respectively), which were evenly distributed throughout the Mackenzie Shelf, and not significantly related to environmental variables. Denaturing gradient gel electrophoresis (DGGE) revealed changes in the bacterial community structure among riverine, estuarine and marine stations, with separation according to temperature and salinity. There was evidence of differences between the particle-associated and free-living bacterial assemblages at the estuarine stations with highest POM content. Particle-associated bacteria are an important functional component of this Arctic ecosystem. Under a warmer climate, they are likely to play an increasing role in coastal biogeochemistry and carbon fluxes as a result of permafrost melting and increased particle transport from the tundra to coastal waters.  相似文献   
4.
Transportation specialists, urban planners, and public health officials have been steadfast in encouraging active modes of transportation over the past decades. Conventional thinking, however, suggests that providing infrastructure for cycling and walking in the form of off-street trails is critically important. An outstanding question in the literature is how one’s travel is affected by the use of such facilities and specifically, the role of distance to the trail in using such facilities. This research describes a highly detailed analysis of use along an off-street facility in Minneapolis, Minnesota, USA. The core questions addressed in this investigation aim to understand relationships between: (1) the propensity of using the trail based on distance from the trip origin and destination, and (2) how far out of their way trail users travel for the benefit of using the trail and explanatory factors for doing so. The data used in the analysis for this research was collected as a human intercept survey along a section of an off-street facility. The analysis demonstrates that a cogent distance decay pattern exists and that the decay function varies by trip purpose. Furthermore, we find that bicyclists travel, on average, 67% longer in order to include the trail facility on their route. The paper concludes by explaining how the distance decay and shortest path versus taken path analysis can aid in the planning and analysis of new trail systems.
Ahmed El-GeneidyEmail:

Kevin J. Krizek    is an Associate Professor of Planning and Design at the University of Colorado where he directs the Active Communities/Transportation Research Group. His research interests include land use-transportation policies and programs that influence household residential location decisions and travel behavior. He has published in the areas of transportation demand management, travel behavior, neighborhood accessibility, and sustainable development. He earned a Ph.D. in Urban Design and Planning and M.S.C.E. from the University of Washington in Seattle. His master’s degree in planning is from the University of North Carolina at Chapel Hill and his undergraduate degree is from Northwestern University. Ahmed El-Geneidy    is a Post-Doctoral research fellow at the Department of Civil Engineering, University of Minnesota and Humphrey Institute of Public Affairs. El-Geneidy’s research interests include transit operations, travel behavior, land use and transportation planning, and accessibility/mobility measures in urban areas. He earned B.S. and M.S. degrees from the Department of Architectural Engineering at the University of Alexandria, Egypt, and continued his academic work at Portland State University, where he received a Graduate GIS Certificate and earned a Ph.D. in Urban Studies from Nohad A. Toulan School of Urban Studies and Planning. Kristin Thompson   was a research assistant with ACT and currently works for Metro Transit in Minneapolis, Minnesota.  相似文献   
5.
We evaluated the phylogenetic diversity of particle-associated and free-living archaeal assemblages from the Mackenzie River and Beaufort Sea in the western Canadian Arctic. The physico-chemical characteristics of the water separated the sampling sites into three groups: riverine, coastal and marine water, which had strikingly different archaeal communities. The riverine water was characterised by the presence of Euryarchaeota mainly belonging to the LDS and RC-V clusters. The coastal water was also dominated by Euryarchaeota but they were mostly affiliated to Group II.a. The marine waters contained most exclusively Crenarchaeota belonging to the Marine Group I.1a. The results suggest that Euryarchaeota in the coastal surface layer are associated with particle-rich waters, while Crenarchaeota are more characteristic of Arctic Ocean waters that have been less influenced by riverine inputs. The particle-associated communities were similar to the free-living ones at the riverine and marine sites but differed from each other at the coastal site in terms of the presence or absence of some taxonomic groups in one of the fractions, or differences in the proportion of the phylogenetic groups. However, there was no specific archaeal group that was exclusively restricted to the free-living or particle fraction, and the diversity of the particle-associated archaeal assemblages did not significantly differ from the diversity of the free-living communities.  相似文献   
6.

Transport related social exclusion is one aspect of people with disabilities’ marginal position in economic, social and civic life. This study aims at presenting main tendencies in empirical research on transport and disability, and therein defining functional requirements that transport solutions must comply with in order to facilitate social inclusion. Based on review of thirty-four empirical studies, this study presents eight functional requirements for inclusive transport. Aspects of each requirement are described and links to social exclusion are explored. Further, the strengths and limits of the review as well as relevance for practice is discussed. Although the study is limited to research in English and Scandinavian languages, the authors believe that the transferability of the results beyond the geographical scope of this study is not challenged by the requirements themselves, but rather to what degree and in what ways these requirements are championed and pursued. The functional requirements are defined without reference to particular travel purposes or mode of transport and represent a systematic approach for making discretional assessment of transport solutions.

  相似文献   
7.
Adapting to changing coastlines is an unprecedented planning challenge as coastal hazards, including those associated with climate change, influence all areas of resource management. Tools have been developed to aid in understanding coastal hazards and to facilitate the planning and implementation of adaptation strategies. Through interviews with members of eight geographically distributed state Coastal Zone Management (CZM) Programs and several federal and nonprofit partners, we explored the utility of current tools for on-the-ground adaptation planning and implementation needs. State CZM Programs operate as trusted information brokers at the nexus between national resources and local needs. Here we provide seven recommendations for how to overcome the challenges identified with tool discovery, lack of trust, and the coarse spatial resolution of national tools. Our findings suggest local community needs are better addressed when end-users play a role in the tool development process. We also recommend leveraging CZM Program connections because they have the knowledge and expertise to identify solutions and disseminate information. While the recommendations presented here were taken from the perspective of state CZM Programs regarding coastal tools, the lessons learned may provide valuable insight into the tool development process for other resource management fields.  相似文献   
8.
In auto-oriented communities, access to an automobile is essential for good mobility, but not everyone owns a car or is able to drive. Little is known about how individuals in these circumstances might still use vehicles for transportation. To provide insight on the nature of vehicle use by those with potentially limited vehicle access, we present qualitative findings from focus groups with recent Mexican immigrants living in California, half of whom owned no cars. Our results demonstrate varying degrees of participants’ access to vehicle travel not always corresponding to auto ownership, with extensive sharing of cars, borrowing of cars, and getting rides. We describe the different dimensions of vehicle access that participants experienced and identify specific factors that seemed to influence their access levels. We discuss the implications of our findings for transportation policy and future research.
Susan HandyEmail:
  相似文献   
9.
Abstract

Disaster research often focuses on how and why communities are affected by a discrete extreme event. We used the community capitals framework to understand how community characteristics influence their preparedness, response to, and recovery from successive or multiple disasters using the 1964 Good Friday Earthquake and the 1989 Exxon Valdez Oil Spill as case studies. This study assesses community response to these disasters by reviewing published literature on impacts to create profiles for six communities and by identifying community capitals before and during these disasters, and throughout the long-term recovery. While the presence of rich natural capitals commonly contributed resources to pre-disaster planning and long-term recovery, restriction of resource access immediately following the disasters was detrimental to many communities. Communities with strong political, social, and financial capitals tended to fare better immediately following disasters, enabling longer-term processes of transformation or recovery. However, in some communities the oil spill undermined these capitals more than the earthquake and resulting tsunami. In understanding how use and reliance on community capitals can lead to varied recovery success from different kinds of disasters, these findings can help coastal managers and planners prepare for future disasters.  相似文献   
10.
Abstract

Community vulnerability is increasingly evaluated through quantitative social indices, typically developed using secondary data sources rather than primary data collection. It is necessary to understand the validity of these indices if they will be used to inform policy and decision making. This paper presents a ground-truthing effort to validate quantitative indices that characterize the well-being of Alaska fishing communities. We utilized ethnographic data collected from 13 representative communities and a capital assets framework to ground-truth the indices, in which qualitative ranks of vulnerability were compared against quantitative indices. The majority (73.8%) of ranks were in complete or moderate agreement and the results indicate that most of the indices are representative of community vulnerability; yet some variables utilized to create the indices could be modified to better reflect realities in Alaska. Indices of commercial fishery engagement and reliance appeared to be more reliable than socio-economic indicators, particularly for smaller fishing communities. We also confirmed that the indices do not capture political, or ecological factors that affect levels of community vulnerability. We conclude that quantitative indices of community vulnerability are useful rapid assessment tools; however, they should be validated, and complemented with ethnographic data prior to their implementation as policy making and management tools.  相似文献   
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