穿错鞋子伤不起

<正>梅西百货的发言人克林顿·凯利说:"鞋子通常奠定了一套服饰的基调。"无论你的衣服多么新潮,穿错的鞋子总是可以瞬间将时尚ICON变身村炮     

细菌对阴极电泳涂装的影响

随着汽车工业的发展,无铅、无锡的环保型阴极电泳漆已经在汽车涂装中获得了广泛应用。实际使用经验表明,由于细菌会严重影响电泳涂膜质量,所以在严格控制无铅、无锡电泳槽液各项工艺参数的同时,还必须对电泳槽内的细菌含量进行监测,通过试验确认了电泳槽内的细菌对电泳涂层有哪些影响,并采取相应措施使问题得到了解决。     

Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection

A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase, equipped with a polyhistidine affinity tag, was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×108, and the specific activity was 2.9×1010 RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein, the ATP content of bacteria was 9.48×10-16mol/mL, and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together, our results provided a simple and efficacious method of the preparation of recombinant luciferase, which could be applied in the determination of bacteria via ATP bioluminescence.     

英国新技术变生活垃圾为汽车燃料

据英国《每日邮报》报道，英国英力士公司（INEOS）日前宣布，他们目前已经掌握了将生活垃圾转变为燃料的技术工艺，并计划到2011年底大规模采用这项工艺生产燃料。 英国英力士公司总裁彼特&#183;威廉姆斯说，他们公司已掌握变垃圾为燃料的生产工艺，并拥有这个生产工艺的专利权。在生产过程中，加热垃圾产生气体，气体和某些细菌反应产生乙醇，乙醇经过净化变成燃料。经过这个生产工艺，1000公斤的垃圾可以生产出400升乙醇。     

Microfluidics-based assay on the effects of microenvironmental geometry and aqueous flow on bacterial adhesion behaviors

Abstract A new microfluidic system with four different microchambers （a circle and three equilateral concave polygons） was designed and fabricated using poly（dimethylsiloxane） （PDMS） and the soft lithography method. Using this microfluidic device at six flow rates （5, 10, 20, 30, 40, and 50 μL/h）, the effects of microenvironmental geometry and aqueous flow on bacterial adhesion behaviors were investigated. Escherichia coli HB101 pGLO, which could produce a green fluorescent protein induced by L-arabinose, was utilized as the model bacteria. The results demonstrated that bacterial adhesion was significantly related to culture time, microenvironment geometry, and aqueous flow rates. Adhered bacterial density increased with the culture time. Initially, the adhesion occurred at the microchamber sides, and then the entire chamber was gradually covered with increased culture time. Adhesion densities in the side zones were larger than those in the center zones because of the lower shearing force in the side zone. Also, the adhesion densities in the complex chambers were larger than those in the simple chambers. At low flow rates, the orientation of adhered bacteria was random and disorderly. At high flow rates, bacterial orientation became close to the streamline and oriented toward the flow direction; All these results implied that bacterial adhesion tended to occur in complicated aqueous flow areas.The present study provided an on-chip flow system for physiological behavior of biological cells, as well as provided a strategic cue for the prevention of bacterial infection and biofilm formation.     

浅谈供水管路中铁细菌的防治与处理

论述了襄樊水电段长江埠地区供水管网中铁细菌对水质污染情况，着重研讨了铁细菌产生原因，提出去除铁细菌的具体措施和预防办法，对今后给水生产具有一定的参考价值。     

多模式区域公交协调调度模型和算法

考虑公交内部及公交和地铁、长途客运之间的换乘衔接问题,满足最小和最大发车间隔等现实约束因素,建立了一类多目标多模式公交协调时刻表模型,在某时段内分别追求非换乘和换乘出行者在所有站点的等车时间最少和所有车辆到达站点时的泊位数最多.利用约束法将该问题转化为单目标规划问题.根据模型特征,设计求解该问题的改进细菌觅食优化算法,定义解的编码方案,设计产生初始种群的启发式算法,将梯度概念引入移动步长进而改进细菌觅食操作.最后,结合一个简单算例,比较单模式和多模式区域公交协调调度之间的差异,分析了站点通行能力对调度结果的影响,并将该算法与其他智能算法进行了比较分析,从而验证了模型和算法的正确性和有效性.     

腹腔外科感染的抗生素疗法

腹腔外科感染病人,抗生素疗法占有重要地位。252例腹腔脓液普通培养,24.2%无菌生长。培养出的菌丛中,绝大多数为革兰氏阴性细菌,大肠艾希氏菌占32.5%。选用抗生素应考虑:①菌丛及其联合存在的多样性;②药物进入胆汁的量与速度;③血与组织中的浓度;④药物的抗菌谱,毒性和价格。     

冬季如何预防车内空气污染?

冬季许多车主喜欢长时间关着车窗开暖风,这使得细菌有机会在车内得以繁衍,而人却毫无察觉.所以,当您感到头疼、头晕、呼吸不适时,最好先关心一下车子的空气质量.