新能源汽车技术专业“1+X”课证融合思考

在《国家职业教育改革实施方案》当中,明确提出了开启"1+X"的证书制度,其主要目的是要利用把学历教育和技能培训进行有效融合,培养出符合时代需求的高素质技术技能人才。以新能源车技术专业作为实例,对"1+X"制度进行积极地探索,找到培养高品质应用型技术人才的新方式,从新能源汽车技术专业的课程内容、考核方式、师资力量等各方面进行探索,为"1+X"课证融合的有效开展寻找高效途径,从而提高人才的质量。     

强化胎盘因子对细胞因子产生的影响

在观察了强化胎盘因子对淋巴细胞增殖试验影响的基础上,应用CTLL-2和7TD_1两种细胞株对强化胎盘因子刺激后的小鼠脾脏细胞分泌白细胞介素-2(IL-2)和白细胞介素-6(IL-6)水平分别进行了实验研究。结果表明,强化胎盘因子促进T淋巴细胞增殖,使小鼠脾脏细胞分泌IL-2和IL-6水牛均明显提高,有增强机体免疫功能的作用。     

IPv4与IPv6的比较研究

文章主要描述了IPv4协议与 IPv6协议的不同之处,分别从首部格式、地址空间、地址分配、路由协议、域名解析、自动配置、安全性等方面对IPv6与IPv4进行了比较研究。指出了IPv6有别于IPv4的新的重要特征。     

中间轴驱动装置固定对弹性悬挂式机车动力学性能的影响

为防止弹性悬挂式机车中间轴驱动装置横向位移过大,发生碰撞,提出了端轴驱动装置弹性悬挂、中间轴驱动装置固定连接的方式。利用多体动力学软件SIMPACK建立整车动力学模型,对比分析驱动装置悬挂方式对机车动力学性能的影响,认为采用这种悬挂方式是合理可行的。     

信号安全技术在DS6-60计算机联锁系统中的主要应用

计算机联锁系统因其应用领域的特殊性,在满足铁路运营需求的同时,必须保证铁路运输的安全。因此对计算机联锁系统的安全性有较严格的要求。广义的信号安全技术包含故障-安全技术、可靠性和易维护性等方面。DS6-60型计算机联锁系统是基于故障-安全技术研发的通过欧标认证的铁路信号安全控制系统。阐述了DS6-60型计算机联锁系统各个环节的主要安全技术应用。     

Full six degrees of freedom coupled dynamic simulation of ship collision and grounding accidents

By taking advantage of the user-defined load subroutine (loadud) and the user common subroutine (usercomm) in LS-DYNA, the authors proposed a new coupled approach for simultaneously calculating structural damage and the planar 3DOF ship motions in ship collisions. The coupled procedure aimed at predicting the detailed structural damage together with reasonable global ship motions. This paper extends the method to consider the full 6DOF ship motions; thus, ship collision as well as grounding accidents can be properly handled. This method is particularly useful for design purposes because the detailed ship hull profile is not needed.A traditional ship maneuvering model is used for the in-plane surge, sway and yaw degrees of freedom with a series of nondimensional coefficients determined from experiments. It is assumed that the out-of-plane degrees of freedom are not coupled with the in-plane ship motions, and there is no coupling among roll, pitch and heave motions. The implementation is verified through free decay tests, and the obtained natural periods show good agreement with theoretical results.Several collision and grounding cases are simulated in which a supply vessel crashes into rigid plates with different orientations. The effects of the roll motion, the heave and pitch motions and the full 6DOF motions are studied. The results are compared with those from a 6DOF decoupled method. Ship motions through the proposed method compare reasonably well with SIMO results. It is found that several consecutive impacts may occur in the simulation of one collision case due to the periodic motions. This is not taken into account in the decoupled method, which makes this method unconservative.     

布达佩斯城市交通发展战略与启示

分别从概况、战略目标、实施策略等3个方面对布达佩斯城市交通发展的总体战略及公共交通、道路网络、静态交通、步行及自行车交通、交通管理等发展战略进行了阐述。由此得出,居民出行线路上任何一种交通方式的低效都将破坏整个交通链的效率,只有整个交通链所有交通环节的和谐发展,才能够使公共交通在运输服务上较小汽车具有竞争力;只有综合利用公共交通与私人交通,才能使城市交通与土地利用相协调。最后,结合布达佩斯和国内实践经验,总结提出了一种包含人口、经济、地理、环境、技术、制度等要素的“六棱形”城市交通发展战略规划模型。     

内蒙古达斡尔族群体9个X-STR位点的遗传多态性及法医学应用价值评估

目的研究内蒙古达斡尔族群体9个X-STR位点(DXS6789、DXS101、DXS8378、DXS7132、DXS7133、DXS7423、DXS6804、DXS6799、HPRTB)的多态性分布及法医学应用价值。方法抽取内蒙古达斡尔族87名健康无关个体静脉血,提取DNA,经PCR扩增、变性聚丙烯酰胺凝胶电泳及银染显色技术进行等位基因分型;采用SPSS13.0软件计算各位点基因型频率和等位基因频率,并检验等位基因分布差异有无统计学意义;Genepop软件进行Hardy-Weinberg平衡检验;Fstat软件计算基因多态性、固定指数并检验固定指数偏离平衡的程度;Powerstats软件计算各种法医学应用指标。结果获得内蒙古达斡尔族群体9个X-STR位点等位基因频率分布的数据;进一步检验获得9个X-STR位点中DXS7133位点的多态性和分化程度较低;DXS7423和DXS7132位点在不同民族中基因分布差异无统计学意义。结论9个X-STR位点中,DXS6789、DXS101、DXS8378、DXS7132、DXS7423、DXS6804、DXS6799、HPRTB 8个位点有较高的遗传多态性和个体识别率,在个体识别和女孩的亲权鉴定中有较高应用价值,对疾病相关研究有实际意义。     

The changes of neuronal activity of the zone incerta in a rodent model of Parkinson＇s disease

Objective To study the changes in neuronal activity of the zone incerta (ZI) following the unilateral lesion of the nigrostriatal pathway. Methods Eiectrophysiological recordings of ZI neurons were made in normal rats and in two groups of rats at different time intervals after injection of 6-hydrodopamine (6-OHDA) into the pars compacta of snbstantia nigra by extracellular recording in vivo. Results The results showed a significant increase in the mean firing rate of ZI during the second and fourth weeks after 6-OHDA lesion [-n=32, (3.6±2. 2)Hz, P<0.001; n=35, (9.3±6.6)Hz, P<0. 001, respectively] compared to that of normal rats [n=39, (9.2±5.2)Hz]. However, no significant change was observed between two groups of 6-OHDA-lesioned rats. With regard to firing pattern, 7.7%(3/39) of ZI neurons discharged regularly, 82.1% (32/39) irregularly and 10. 3% (4/39) in bursts in normal rats. During the second week after 6-OHDA lesion, the regular, irregular and bursting firing neurons recorded in the ZI were 9.4% (3/32), 59. 4% (19/32) and 31.3% (10/32), and during fourth week, the regular, irregular and bursting firing neurons were 14.3% (5/35), 57.1% (20/35) and 28. 6% (10/35), respectively. The firing pattern of the neurons in the three different groups did not change significantly. Conclusion These results suggest that the firing rate of ZI neurons in 6-OHDA-lesioned rats is increased significantly, which may contribute to the pathophysiology of Parkinson's disease.     

弥漫性轴索损伤后CD4~+/CD8~+ T细胞比值、IL-6、IL-10水平变化及预后相关危险因素的Logistic回归分析

目的研究弥漫性轴索损伤(DAI)后患者外周血CD4+/CD8+T细胞比值及炎症因子IL-6、IL-10水平的变化,探讨影响DAI患者预后的相关危险因素及可能的预测因子。方法收集115例DAI患者的相关临床资料及伤后至少6个月的随访结果,并将采集的患者外周血采用流式细胞仪检测CD4+、CD8+T细胞及其比值,ELISA法检测IL-6、IL-10水平,对免疫炎症指标及临床资料进行单因素和多因素分析,多因素分析采用Logistic回归分析。结果DAI后合并其他脑损伤、瞳孔改变、伤后昏迷时间及入院时GCS评分是影响患者预后的危险因素,而其他因素则与预后不相关。CD4+/CD8+T淋巴细胞比值降低是DAI后发生ARDS/ALI的危险因素,CD4+/CD8+T淋巴细胞比值与患者预后关系不大,而ARDS/ALI的发生则是影响DAI患者预后的重要危险因素。IL-6增加与DAI后ARDS/ALI密切相关,可作为ARDS/ALI发生的预测因子,其水平是患者预后的危险因素,而IL-10则在此过程中与ARDS/ALI不相关,且与患者预后无关。结论 CD4+/CD8+T淋巴细胞比值降低是DAI后发生ARDS/ALI的危险因素,IL-6可作为ARDS/ALI发生的预测因子。DAI后与患者预后相关的危险因素包括合并其他脑损伤、瞳孔改变、伤后昏迷时间、入院时GCS评分、IL-6水平及并发ARDS/ALI。