汽车电气系统研究与探讨

对车载电气系统用电量迅速增加的趋势进行了讨论，阐述了提高车载电气系统电压值的解决途径和方案，采用42V电压值的车载电气系统既能满足电器和电子设备用电功率增加的需要，又便于使用现代技术对电子、电器元件和人身进行有效保护。给出了42V电压值车载电气系统的结构布置方案，并对其实施时间作了预测。分析了采用42V电压值的车载电气系统面临的挑战和发展动向。     

42 V电控发动机冷却系统的研究

冷却系统是发动机的重要组成部分,分析了传统冷却系统的不足,设计基于模糊控制的42V发动机电子控制冷却系统,该冷却系统能够实现散热风扇的无级变速,使发动机工作在最佳温度,使之更经济、更环保,给出了模糊控制器的仿真结果,对比试验结果显示较传统冷却系统性能优越。     

siRNA沉默MAPK p42对肝癌SMMC7721增殖和凋亡的影响

目的应用siRNA沉默SMMC7721细胞MAPK p42,并观察其对增殖和凋亡的影响。方法应用SilencerTMsiRNA构建试剂盒合成2条MAPK p42siRNAs和一条随机对照siRNAs,用脂质体LipofectaminTM 2000将其转染入肝癌SMMC-7721细胞株。应用Western blot检测MAPK p42表达,MTT法检测细胞增殖情况,流式细胞仪分析细胞周期及其细胞凋亡。结果与对照相比,MAPK p42siRNA明显抑制MAPK p42表达。MAPK p42siRNA抑制了SMMC-7721细胞的生长,将细胞阻滞于细胞周期S期,进而诱导了细胞凋亡的发生。结论 MAPK p42靶向siRNA可能为肝癌基因治疗的重要手段。     

β淀粉样肽1～42单克隆抗体的制备

目的　制备稳定分泌β -淀粉样肽 1～ 4 2 (Aβ1-42 )单克隆抗体的杂交瘤细胞 ,产生高效价抗体。方法　通过基因工程技术 ,将Aβ1-42 基因融合于乙肝核心抗原 (HBcAg)的主要免疫优势区 (MIR) ,制备Aβ和HBcAg的融合蛋白 ,免疫BALB/c小鼠 ,取脾细胞与SP2 / 0细胞融合 ,筛选能稳定分泌Aβ1-42 单克隆抗体的杂交瘤细胞。结果　得到两株能稳定分泌Aβ1-42 单克隆抗体的杂交瘤细胞 ,Aβ1-42 单克隆抗体的Ig亚类为IgG3 。结论　制备得到的Aβ1-42 单克隆抗体特异性强 ,效价高     

汽车电源电压标准提升的研究

随着电子、电器元件的发展,其在汽车上的应用日益广泛,以致汽车原有的电能供应出现了严重不足,提高汽车供电系统的电压,采用42V电压值,已成为一些国际论坛中的讨论热点。本文以陕汽牌SX2190越野车为例,并结合当前汽车行业的发展现状,对提高汽车供电系统电压进行研究。     

现代汽车电压升级方案与发展趋势

14V电源系统已不能满足汽车电气设备日益增长的需要，汽车电压升级势在必行，文章阐述了两种汽车电压升级方案的原理和优缺点，介绍了42V汽车电源系统 的发展状况及我国应采取的对策。     

Surface chlorophyll in the Black Sea over 1978–1986 derived from satellite and in situ data

Absolute values of chlorophyll a concentration and its spatial and seasonal variations in the Black Sea were assessed by using satellite CZCS and in situ data. Since the satellite CZCS had operated for the 1978–1986 period, CZCS data was used for assessing the past state of the Black Sea just before the onset of drastic changes observed in late 1980s. The approach used for the calculation of the absolute values of chlorophyll a concentration from CZCS data was based on the direct comparison of in situ chlorophyll a data and those of CZCS and by applying the algorithm developed for the transformation of CZCS data into chlorophyll a values. CZCS Level 2 data related with pigment concentration having a spatial resolution of 1 km at nadir were used. The daily Level 3 files were derived by binning Level 2 values into 4-km grid cells and the monthly and seasonal Level 3 files were created by averaging the daily Level 3 files over the corresponding period. In situ chlorophyll a data were obtained by spectrophotometric and fluorometric methods in 15 scientific cruises over the 1978–1986 period. Total number of ship-measured data used for the comparison with those CZCS values was 590.Chlorophyll a concentration (Chl) was derived from CZCS values (C) with regression equations Chl=kC; the coefficient of transformation k was calculated from six different data sets by taking into account distinctions between subregions and seasons. The reasons for difference in the k values have been analyzed.Statistical comparison of the chlorophyll a values measured in situ and those derived from CZCS data was based on log-transformed data and gave the following results: regression SLOPE=0.842, regression INTERCEPT=−0.081, coefficient of determination (R²)=0.806, root–mean–square ERROR=0.195. The mean monthly chlorophyll a distributions derived from CZCS data over 1978–1986 have been constructed and the mean seasonal chlorophyll a values in different regions have been calculated and analyzed. The significant difference in chlorophyll concentration between the western shelf regions and the open part of the Black Sea has been demonstrated, especially in warm season. At almost all seasons, the highest chlorophyll concentration is observed in the western interior shelf region which is under strong influence of Danube. The summer mean chlorophyll concentration in this region is 18 times higher than that in the open parts and about nine times higher than in the eastern shelf region. The greatest seasonal variations are observed in the open part of the Black Sea: chlorophyll concentration in cold season is four to six times higher than in summer and three to five times higher than in April and October. To the contrary, in the western interior shelf regions, the concentration is higher in May–October (about twice than that in November–March). Seasonal variations in the western outer shelf regions are smoothed out as compared with both the western interior shelf and the open regions.     

汽车42V／14V双层直流转换充电器的设计

本文设计了42V电气系统网络架构,结合直流转换器在该架构中的功能定义,设计了同时为负载供电和为蓄电池充电的直流转换器。选用功能完善的PWM控制及驱动器TL1451A,并考虑到实际功率需求,转换器采用新的拓扑结构,设计了双层降压转换器以提高功率。经测试,此设计能满足功能和功率需求。     

Determining optimal inland-empty-container depot locations under stochastic demand

Maritime freight volumes fluctuate with changes in consumer demand and global economies. In this paper we analyze the stochasticity in the container trade volumes and perform a two-stage stochastic analysis with recourse to determine an optimal set of inland depots to be opened within a 10-year time horizon. The work builds on our previous effort that introduced the concept of an ‘Inland-Depots-for-Empty-Containers’ (IDEC) system for effective regional repositioning of empty containers. We evaluate the proposed approach by performing a case study based on the port region of New York/New Jersey. Results obtained demonstrate greater expected benefit from the proposed approach as compared to the corresponding deterministic or single-stage stochastic models.     

MAN B&W 6S42MC7主机滑油压力偏高的原因分析

根据32000DWT散货船主机MAN B&W 6S42MC7出现滑油压力偏高的现象,并通过理论计算进行论证,结果表明,理论计算与现场测量结果基本吻合,同时采取了增加流量调节阀的改进措施。