表皮基底上层变异维甲酸X受体转基因鼠抑制表皮基层角朊细胞增殖

为了解维甲酸受体RXRα在活体表面角朊细胞增殖分化中的作用，对缺AF－2激活功能段的RXRα变异基因及仅在表皮基底上层表达的牛角蛋白10作为启动子的转基因鼠进行研究。发现鼠表皮发育无异常。以tRA、人工合成选择结合于RAR的CD367分别局部处理后，CRABPⅡ、CRBPⅠ、CRBPⅡmRNA的诱导表达明显低于正常鼠．表皮增厚及基层细胞有丝分裂也明显减少。而对人工合成选择结合于、RXR的SR11237无反应。说明体内表皮维甲酸受体以RARγ／RXRα条合双倍体形式为主；表皮基底上层细胞通过维甲酸细胞间信号传递可调控基层细胞的增殖。     

皮肤衰老进程中大鼠角质形成细胞的凋亡及松花粉的干预作用

目的观察衰老进程中不同年龄组大鼠角质形成细胞的凋亡率及松花粉对老年组大鼠角质形成细胞凋亡的干预作用。方法以大鼠皮肤角质形成细胞为研究对象,采用末端脱氧核苷酸转移酶介导的dUTP原位切口末端标记(TUNEL)技术检测幼年组、成年组、老年组以及松花粉喂养老年组大鼠角质形成细胞的凋亡情况。结果幼年组、成年组、老年组、松花粉喂养老年组的凋亡率分别为:(73.549 50±4.437 68)%(、59.509 10±4.781 50)%(、88.978 00±3.702 25)%(、61.820 00±7.784 60)%。幼年组的凋亡率介于成年组与老年组之间,老年组的调亡率最高(P<0.01)。松花粉干预后,松花粉喂养老年组的凋亡率比老年组明显下降(P<0.01)。结论随着年龄增加,大鼠角质形成细胞的凋亡率逐渐增加,且松花粉对细胞凋亡有一定的干预作用。     

依曲替酸对角质形成细胞肝素结合表皮生长因子样生长因子mRNA的影响

目的研究依曲替酸对培养的正常人角质形成细胞(KC)增殖和肝素结合表皮生长因子样生长因子(HB-EGF)mRNA表达的影响。方法0.1和1μmol/L伊曲替酸处理正常人KC 12 h后,用噻唑蓝比色法(MTT法)和实时荧光定量逆转录聚合酶链反应(RT-PCR)法分别检测KC增殖和HB-EGF mRNA表达的改变。结果依曲替酸处理12 h后,可剂量依赖抑制KC的增殖和上调HB-EGF mRNA的表达。0.1和1μmol/L伊曲替酸分别使KC增殖抑制10.2%和14.4%,使HB-EGF mRNA增加到正常对照组的3.2和7.1倍。与对照组相比,差异均有统计学意义(P<0.05)。结论依曲替酸可剂量依赖上调HB-EGF mRNA的表达,其可能参与依曲替酸对KC增殖的抑制作用。     

靶向间皮角质细胞生长因子对术后腹膜粘连的预防作用

目的研究角质细胞生长因子(KGF)对大鼠腹膜粘连形成的预防作用及对腹膜粘连组织中组织纤溶酶原激活物(tPA)和纤溶酶原激活物抑制剂(PAI-1)表达的影响。方法 SD大鼠30只,随机分为3组(n=10),分别为KGF组、模型组和假手术组。7 d后处死大鼠,并参照修改后的Leach等评分系统评价粘连程度。光镜观察组织形态学变化,并用免疫组化SP法测定腹膜粘连组织中tPA和PAI-1表达情况。结果 KGF组粘连总评分结果(4.8±0.92)明显低于模型组(7.6±1.35)(P<0.01),免疫组化染色示KGF组tPA表达水平(88.41±3.92)明显高于模型组(111.63±4.04)和假手术组(100.91±1.57)(P<0.01),而PAI-1表达水平在3组之间无差异(P>0.05)。结论 KGF通过促使损伤处腹膜再生及纤溶能力恢复达到预防腹膜粘连形成,提示间皮再生可能是预防粘连形成的有效途径。     

Biological properties of differently-aged human keratinocytes.population doubling time growth curve and cell cycle analysis

Objective To explore the biological properties of keratinocytes from differently-aged healthy human beings. Methods Keratinocytes from fetus, teenager and middle-aged groups were separated and cultured. The population doubling time (PDT) and cell growth curve in different cells were compared, and the cell cycles were analyzed by flow cytometry. Results ① In primary culture of keratinocytes, the adherence time in middle-aged group was longer than that in fetus and teenager groups. However, all cell morphology showed no obvioas differences. In subculture of kecatinocytes, with donator's age increasing, time of cell adherence prolonged, passage number decreused and differences in cell morphology were obrioas. ② The average PDT of keratinocytes was shorter in fetus group than in teenager and middle-aged groups. Bat difference in cell growth curve between different passages was not observed. ③ Keratinocytes showed G2/M period in fetus group but G0/G1 period in teenager and middle-aged groups mainly. Conclusion As age increases, the biological properties of keratinocytes change obviously.     

窄谱中波紫外线对角质形成细胞肝素结合表皮生长因子样生长因子mRNA的影响

目的研究窄谱中波紫外线(NB-UVB)对培养的正常人角质形成细胞(KC)增殖和肝素结合表皮生长因子样生长因子(HB-EGF)mRNA表达的影响。方法用50 mJ/cm2和100 mJ/cm2NB-UVB照射正常人KC后继续培养12 h,用MTT法和实时荧光定量RT-PCR法分别检测KC增殖和HB-EGF mRNA的改变。结果NB-UVB照射正常人KC后,可剂量依赖抑制KC的增殖和上调HB-EGF mRNA的表达。50 mJ/cm2和100 mJ/cm2NB-UVB照射后,分别使KC增殖抑制5.4%和8.7%,使HB-EGF mRNA增加到正常对照组的1.7倍和2.5倍。结论NB-UVB抑制KC的增殖部分是通过上调HB-EGF的表达介导的。