刀豆蛋白A条件化培养基对体外培养的兔角膜内皮细胞生长的影响

目的研究刀豆蛋白A(concanavalin A,ConA)条件化培养基对体外培养的兔角膜内皮细胞(rabbit corneal endothelial cells,RCECs)生长的影响。方法采用揭取后弹力层及内皮细胞联合组织块法进行RCECs的体外原代培养,分别用含有体积分数为5%、10%、15%、20%ConA的活化大鼠脾细胞条件上清液的RPMI1640培养基进行RCECs原代培养,并用含10%(体积分数)胎牛血清(fetal bovine serum,FBS)的RPMI1640培养基作为对照。神经烯醇化酶免疫组织化学染色法进行细胞鉴定;噻唑蓝比色法观察各组RCECs生长的状况;SPSS11.0One-way ANOVA及LSD检验进行统计分析。结果成功进行了RCECs的体外培养,培养细胞内神经烯醇化酶阳性表达。ConA条件化培养基各组细胞增殖均高于对照组(P<0.001),且10%、15%ConA条件化培养基组的RCECs细胞数量明显高于其他各实验组(P<0.001)。结论ConA条件化培养基具有促进RCECs生长的作用,可作为培养辅佐剂。     

柴油机燃用麻疯树油甲酯的氮氧化物排放特性

研究了柴油机燃用麻疯树油甲酯的NOx排放特性。以1台轻型车用直喷式柴油机为试验样机,分别燃用6种不同生物柴油掺混比例的混合燃油,研究总的NOx排放以及NO,NO2,N2O等NOx主要组分的排放特性。结果表明,燃用各种混合燃油的NOx排放曲线形态较为接近,低负荷时差异较小,随负荷增加,排放差异增大。NOx排放以NO和NO2为主,NO排放随着负荷上升而增加,NO在总NOx排放中始终占有最高比例。NO2排放也占有相当比例,在低负荷时较高,随着负荷增大浓度降低,大负荷高温不利于NO2的生成。N2O排放量极低,在中低负荷时有一定生成量,高负荷N2O排放几乎为0,缸内稀燃低温有利于N2O排放的生成。在同一稳定工况下,随生物柴油混合比的提高,NOx,NO,NO2比排放量呈线性增加,N2O比排放量呈线性降低。发动机燃烧生物柴油后,NOx及其组分NO,NO2和N2O的排放量发生改变,而各自的排放变化规律并未发生变化。     

Fabrication of a Novel Porous Poly（Vinylidene Fluoride） Blend Five-Bore Membrane for Wastewater Treatment

Porous poly(vinylidene fluoride) (PVDF)/poly(methyl methacrylate) (PMMA) blend five-bore hollow fiber membranes are prepared by wet phase inversion methods. In spinning these PVDF hollow fibers, N, N-dimethylacetamide (DMAc) and polyvinyl pyrrolidone (PVP) are used as the solvent and the non-solvent additive, respectively. The external coagulant uses water. The internal coagulants use water and DMAc-water solutions. The membranes are characterized in terms of water flux and molecular weight cut-off for the wet membranes. The cross-sectional structures are examined by scanning electron microscopy (SEM). The effects of polymer concentration and the internal coagulant on the permeation properties and membrane structures are examined keeping the bore liquid flow and air-gap constants. Relative high flux, rejection and strengh PVDF five-bore hollow fiber membranes could be prepared from the polymer concentration in dope solution at 17% of mass fraction, the air gap distance of 14 cm, and using 10% of mass fraction of DMAc solution as the internal coagulant at 13mL/min of flow.     

水泥混凝土桥面铺装长效防水粘结体系MMA性能研究

在我国为数众多的水泥混凝土桥梁在远未达到设计寿命时,就出现了钢筋锈蚀、混凝土松散、结构强度下降、承载能力大幅衰减等病害现象。桥面铺装防水粘结体系的性能对桥面铺装的使用品质及对水泥混凝土桥梁结构的使用寿命具有显著影响。设置长效防水粘结层是确保桥面铺装结构耐久性的重大措施之一。以湖南省娄新高速公路水泥混凝土桥桥面铺装防水粘结层设置为实际研究背景,引用国外先进的水泥混凝土桥面铺装防水粘结体系设计方法及性能评价体系,对国外应用广泛且优良的长效甲基丙烯酸甲酯 MMA 防水粘结体系与我国普遍采用的 SBS 改性沥青碎石封层、改性乳化沥青等防水粘结材料防水进行了对比试验,为深入进行湖南省娄新高速公路水泥混凝土桥面铺装防水粘结体系研究及实际工程选择应用提供理论依据。     

纤维沥青混合料试验性能研究

在AC、SMA、OGFC 3种级配形式的沥青混合料中分别掺加国产聚酯纤维、聚丙烯腈纤维和木质素纤维,测定纤维沥青混合料的路用性能,包括混合料马歇尔稳定度、高温稳定性、低温抗裂性、水稳性、渗水性和抗滑性能,分析了纤维增强沥青混合料强度形成机理.与无纤维沥青混合料试验结果进行对比,3种纤维沥青混合料的路用性能都有不同程度的提高,聚酯纤维与聚丙烯腈纤维的综合改善性能优于木质素纤维.通过车辙试验,确定了聚酯纤维满足不同交通量下的设计用量.技术经济分析表明,纤维沥青混合料经济效益明显,具有良好的应用前景.     

纳米氯化银胶体的光催化降解性能研究

该文指出,甲基橙是一种酸碱指示剂,其分子式:C14H14N2NaO3S,性状为橙黄色粉未或片状结晶,易溶于热水,几乎不溶于乙醇。其分子结构与颜色均随溶液pH值的变化而变化,它具有相对较高的毒性、复杂的结构和难于生物降解性,因此在降解甲基橙溶液有机废水处理中具有典型意义。该文着重介绍了纳米氯化银胶体的光催化降解性能。     

UHMWPE-45#钢用改性氯丁橡胶粘合剂的研究

采用甲基丙烯酸甲酯（MMA）对氯丁橡胶（CR）进行接枝改性,并分别采用正交试验设计方法和一种新的工业过程操作优化方法——可视化优化方法对合成工艺条件进行分析处理、预测和优化;并对胶膜的性能进行分析.结果表明：影响拉伸剪切强度因素主次顺序依次为,MMA浓度、BPO浓度、溶剂量、反应温度、反应时间;剪切强度随着接枝率的增大而增强;最佳工艺条件为,CR100份、MMA60份、混合溶剂700份、BPO1.0份、温度82.5℃、反应时间4h,制得的CR-MMA胶接枝率达39.57％、对UHMWPE和45#钢的粘接强度为0.823 4 MPa;MMA接枝改性破坏CR分子结构排列的规整性,改善了CR胶的耐热性,使CR-MMA胶黏剂的耐热温度可达200℃以上.     

木质素纤维投放机的优化设计与改造

针对目前沥青拌和站使用的木质素纤维投放设备存在的不足,结合自身实践,对纤维吹送设备结构不合理等缺陷和问题进行了改造.实践证明,改造后的设备纤维投放时间缩短、精度增加,生产效率大幅提高.     

THE ROLE OF NITRIC OXIDE IN SMALL INTESTINAL MIGRATING MOTOR COMPLEX

Theinterdigestivemigratingmotorcomplex(MMC)inthesmallintestineisknownasaphasiccontractiontl).ThreepiecesofmajormechanismofinitiationoftheMMChavebeeninvestigated:extrinsicneuralcontrol,hormonalcontrol,andintrinsicneuralcontrol.Theinitiationandmigratingismainlycontrolledbythehormonalandtheintrinsicneural,thecentralnervoussystemregulatestheMMCcyclingonlyduringfed,sleepandstressstates.Nitricoxide(NO)hasbeenfoundoneoftheinhibitoryneurotransmitterofnonadrenergicnoncholinergic(NANC)neuronsinthe…     

The role of nano additives for biodiesel and diesel blended transportation fuels

The energy crisis is due to two reasons, one is the rapid increase in worldwide population and the other is changing living style of human beings. The fossil fuel is also a major contributor to add the harmful pollutants into the atmosphere. Fuel modifications play a major role in increasing engine efficiency and reducing emissions. In the present investigation focused on fuel modifications in diesel engine. Initially the single cylinder diesel engine was operated with 20MEOM, 40MEOM, 60MEOM, 8MEOM and 100MEOM without additives with diesel at different loads at constant rated speed. From the experimental study proved that 20MEOM is the best fuel ratio compared to other blends. In second phase based upon first phase results the engine was operated 20MEOM blended fuel with adding 50 ppm copper oxide nano additives with diesel using solgel process. From the results, the brake thermal efficiency was 2.19% improved compared than 20MEOM blend without additive at full load condition. Emissions of HC, CO and smoke were considerably reduced. The present analysis reveals that the biofuel from mahua oil with nano additives is quite suitable as an alternate fuel for diesel engine.