姜黄素促进RAW264.7源性M1巨噬细胞向替代激活M2表型极化

目的观察姜黄素对LPS和IFNγ诱导的RAW264.7巨噬细胞(M1)极化的影响及机制。方法不同浓度姜黄素(6.25、12.5、25μmol/L)干预LPS和IFNγ诱导的RAW264.7巨噬细胞(M1)12h,同时再加入20μmol/L GW9662与25μmol/L姜黄素共同干预LPS和IFNγ诱导的RAW264.7巨噬细胞(M1)12h,采用Real-time PCR、ELISA及Western blot方法检测IL-1β、IL-6和M2表型标志分子KLF4、FIZZ1、MGL1、PPARγ的表达,及阻断PPARγ后KLF4和FIZZ1的表达。结果不同浓度姜黄素均能上调LPS和IFNγ诱导的RAW264.7巨噬细胞(M1)的M2标志分子的表达,并且抑制炎症因子IL-1β和IL-6的分泌;阻断PPARγ后,RAW264.7巨噬细胞源性M1表型巨噬细胞表达M2标志分子下调。结论姜黄素通过活化PPARγ促进LPS和IFNγ诱导的RAW264.7巨噬细胞(M1)向M2表型极化。     

基于网络构建方法探究遗传疾病的发病机制

基于网络构建的方法构建疾病表型相关网络,并对网络作拓扑学结构和功能分析。结果提示表型相关的疾病在分子层面上有一定的相似性,这为相关疾病药物靶标的选择和个性化治疗提供一定的临床指导意义。     

OCCURRENCE CHARACTERISTIC OF KASHIN-BECK DISEASE BASED ON NUCLEAR FAMILY PEDIGREES

Kashin Beckdisease (KBD )isanendemicos teochondropathyresultingfromimpairedendochon drialdevelopmentinchildren .Itmanifestswithsecondarychronicdeformitiesandosteoarthrosisinadults.Geographically ,thediseaseoccursmainlyinadiagonalbeltrangingfromthenortheasttothesouthwestofChina (KBDarea) .Thisareaischar acterizedbyalowfoodseleniumcontent .Thereare2 .5millionpatientswithKBDand 30millionpeo pleareatrisk[1] .TheclinicalmanifestationsofKBDareflexionoftheterminalpartofthefingersorcrookedfinger…     

Association between the -1562 C/T polymorphism in the MMP-9 promoter and phenotype of esophageal squamous cell carcinoma in northern Chinese population

Objective To conduct a case-control study on the association of the nucleotide polymorphisms in the promoter region of the matrix metalloproteinase-9 (MMP-9) gene with phenotype of esophageal cancer. Methods All subjects were unrelated residents in northern regions of China. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was used to determine the MMP-9 genotypes. Results The overall distribution of genotypes in the patients was not different from that in the controls (OR=0.77, 95% CI=0.45-1.34; P=0.36). There were no significant differences between the patients and the control subjects in terms of the distributions of sex and age, smoking status, alcohol dependence, pickled diet status, or history of environmental exposure. The patients were further examined with stratifications by age, sex, grade, depth of tumor invasion, lymphatic invasion, venous invasion and TNM staging. The results showed no pronounced association among the stratifications. Conclusion There is no significant association between the MMP-9 single nucleotide polymorphism genotypes and phenotype of esophageal cancer.