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Objective A method of TLC-fluorescence spectrophotometry was established to assay the content of polydatin in polygonum cuspidatum sieb. et zucc. Methods: Polydatin was extracted by methanol and separated with ch‘loroform-acetone-formic acid-water (4:4:0.5:0.2) by thin layer chromatography. The excitation wavelength and emission wavelength were 284 nm and 384 nm, respectively. Results The linear regression equation of the calibrationgraph was y=7.02179x 4.5143, a linear regression correlative coefficient r= 0. 9936. Conclusion This method was proved simple, stable and sensitive. It can be used in quality control of herbs. 相似文献
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