几类水产动物血细胞免疫研究现状

综述了鱼类,甲壳类,贝类水产动物的血细胞免疫研究进展,为进一步了解血细胞在正常的生理活动中和一些疾病病理生理发展过程中的作用、探讨血细胞的免疫调节机制以及其它相关研究提供参考。     

Effect of elastic constitutive laws on the deformation of two-dimensional red blood cell

Deformation of two-dimensional red blood cell in linear shear flow is simulated using the immersed boundary method, in which the cell is modeled as a force source instead of a real body. The effect of three constitutive laws, i.e. Hookean, Neo-Hookean and Skalak elasticity, on the deformation is studied by simulating the cell movement in two linear shear flows. The results show that the effect of the constitutive laws gets more obvious as the shear rate increases. Both the aspect ratio and the inclination of the steady shapes get bigger, and the differences between the periods of the cell tank-treading motion become larger. For the same shear flow, the period with Hookean elasticity is less than the period with Neo-Hookean elasticity and bigger than the period with Skalak elasticity.     

Toxicity to the normal hemocytes by ALA-PDT for the ex vivo purging of hematopoietic stem cell grafts

Objective To study the toxic effects of 5-amionlevulinic acid-based photodynamic therapy (ALA-PDT) on human peripheral blood mononuclear cells (PBMCs), cord blood mononuclear cells (CBMCs) and peripheral blood stem cells (PBSCs), and furthermore, to understand the possible causes of this response. Methods We used MTT assay to detect the survival rate of PBMCs, CBMCs and PBSCs after treated by ALA-PDT under the optimum experiment conditions with U937 as control;Annexin V-FITC/PI was used to detect the pattern of cell death induced by ALA-PDT. By using flow cytometry, we detected intracellular PpIX fluorescence intensity. Results After ALA-PDT treatment the survival rate of PBMCs had no significant change;however in PBSCs and CBMCs, the survival rate reduced to 70%, and the survival rate of leukemia cell U937 was the lowest, about 30%. After incubation with ALA,except for PBMCs, intraceUuiar PplX fluorescence intensity of the other two kinds of normal haemocytes and U937 increased obviously. These results combined with the flow cytometry suggested that the main pattern of cell death here was apoptosts. Conclusion Under the optimum experiment conditions, ALA-PDT has a slight effect on normal haemocytes but excellent depletions of leukemia cells. Therefore, it can effectively purify autologons bone marrow or stem cell grafts.     

单核吞噬细胞与造血调控

单核吞噬细胞是人体重要的免疫活性细胞。它在吞噬并杀伤病原生物、提呈抗原参于免疫反应及分泌生物活性物质以调节机体多种生理功能方面的作用已逐步为人们认识,而且随着对单核吞噬细胞研究的进一步深入,上述作用的内涵也在不断更新。近些年来,人们发现单核吞噬细胞还与造血调     

当心开车、装修等带来的血液病

<正>开新车开出白血病,染发和装修引起血液病、缺铁性贫血患者日渐增加……这些都是现代生活给人们带来的祸端。如今,开车一族的队伍越来越壮大,接触汽油的人群也随之越来越多。因汽油中含有导致血液病的苯物质,所以开车族中发生血液病的机会大大增加。有关专家提醒,避免这种可能性的办法就是尽量减少汽油外泄。另外,长期开车的人不妨经常到医院化验一下血常规,如果开车后白细胞明显下降,又不     

高压氧对荷S—180瘤细胞小鼠血液某些指标的影响（论著）

目的：探讨高压氧（ＨＢＯ）暴露对荷瘤小鼠血液指标的影响。方法：用雄性Ｂａｌｂ／Ｃ小鼠２０只，随机分成４组，每组５只。Ａ组为正常对照；Ｂ组只行ＨＢＯ暴露；Ｃ组只做肿瘤接种；Ｄ组行ＨＢＯ暴露并接种肿瘤。Ｂ、Ｄ组经压力０．２ＭＰａ、氧浓度８７％、氧分压０．１７４ＭＰａ的ＨＢＯ暴露２０次后，所有鼠摘眼球取血对其血红蛋白、白细胞总数、中性白细胞数、淋巴细胞数、中性白细胞百分比、淋巴细胞百分比作了观察。结果：