外源性p27Kip1对兔眼滤过术后结膜瘢痕的抑制作用

目的观察外源性p27Kip1作用于兔眼滤过术后的效果,研究其对术后结膜瘢痕形成的抑制作用。方法根据GenBank人p27基因序列设计引物,制备腺病毒介导Ad-p27并验证其正确性。兔眼滤过术中结膜下注射Ad-p27、丝裂霉素C(MMC)和PBS,术后28d内测定眼压、观察眼前节的动态变化及并发症,依照Krofeld评分法进行滤过泡评分。结果 Ad-p27作用后28d内维持低眼压的状态,术后3d眼压下降达峰值7.68mmHg。Ad-p27降眼压程度与MMC相似,与PBS比较术后7d(P<0.05)、14d(P<0.01)、21d(P<0.01)和28d(P<0.05)眼压存在显著性差异。Ad-p27作用后14d内滤过泡弥漫性隆起,28d有囊性泡形成。Ad-p27组与PBS组比较,滤过泡评分在7d(P<0.01)、14d(P<0.01)和21d(P<0.05)有显著性差异。Ad-p27组术后21d前房恢复正常,而PBS组术后14d恢复正常。Ad-p27作用偶有前房渗出、前房出血等并发症。结论 Ad-p27通过抑制手术区瘢痕形成保持滤过道通畅,降低眼压。     

CyclinD1和P27蛋白在人脑胶质瘤中的表达及其意义

目的　探讨人脑胶质瘤组织中CyclinD1和P2 7蛋白的表达及其相互关系。方法　采用SP免疫组化技术检测脑胶质瘤和非肿瘤组织中两者的表达 ,并用彩色图像分析系统作定量分析。结果　①两者的免疫反应复合物均定位于细胞核。②两者在脑胶质瘤中的表达均显著高于非肿瘤组织 ,且CyclinD1蛋白的表达强度随肿瘤恶性程度增加而升高 ,而P2 7蛋白的表达强度则随肿瘤恶性程度增高而下降 ,两者的表达呈显著负相关。③CyclinD1蛋白的表达强度愈高则预后愈差 ,而P2 7蛋白的表达强度愈低则预后愈差。结论　两者的异常表达可能与人脑胶质瘤的发生和发展有密切关系 ,且具有协同作用 ,其表达强度可客观地反映肿瘤的恶性程度和评价预后。     

P27KIPL蛋白在卵巢浆液性肿瘤中的表达及意义

目的　探讨P2 7KIPL蛋白在卵巢浆液性肿瘤中的表达及其与生物学行为和预后的关系。方法　采用免疫组化SP法检测 88例卵巢浆液性肿瘤中P2 7KIPL蛋白的表达水平。结果　 6 1例卵巢浆液性囊腺癌中P2 7KIPL阳性表达率为 4 6 % (2 8/ 6 1例 ) ,明显低于浆液性囊腺瘤 80 % (12 / 15例 )和浆液性交界性肿瘤 75 % (9/ 12例 ) (P <0 0 5 )。P2 7KIPL阳性表达在Ⅰ级癌中表达率最高 73 3% ,Ⅱ级癌 4 6 7% ,Ⅲ级癌表达最低 12 5 %。三组之间差异显著 (P <0 0 5 ) ,P2 7KIPL阳性表达与组织分级呈负相关。早期 (Ⅰ Ⅱ期 )P2 7KIPL表达率 76 % ,明显高于晚期 (Ⅲ Ⅳ期 ) 2 7% (P <0 0 5 )。结论　P2 7KIPL蛋白表达水平可能与卵巢浆液性囊性癌组织分化、临床分期有关。     

Al-27Zn合金固溶时效后硬度及耐磨性研究

研究了两种Al-27Zn合金经425℃×8h固溶及125℃时效处理后组织结构、硬度和耐磨性的变化规律.结果表明,经时效处理后合金的组织形态为：Al基体上均匀分布着少量未溶树枝状晶组织以及大量细小弥散富锌的η相;合金的硬度随着时效时间的延长先升后降,合金元素Cu、Mg的加入提高了Al-27Zn的硬度;两种合金均具有良好的耐磨性,但合金元素Cu、Mg的加入降低了耐磨性;探讨了磨损机制及材料组织、硬度对合金耐磨性的影响.     

27t轴重通用货车应用下调车场连挂区调速控制研究

27 t轴重通用货车的应用给调车场连挂区调速控制提出了新问题。分析27 t轴重通用货车单位基本阻力更小、溜放动能更大等特点,减速顶对27 t轴重通用货车制动功的减小、临界制动速度的上浮等特性,以及与既有货车混合应用后超速连挂风险的增加、调速控制复杂性的增大等问题。通过模型建立、参数标定及仿真计算,得到减速顶对27 t轴重通用货车制动功的减幅及临界制动速度的漂移量。为解决27 t轴重与既有货车混合应用下调车场连挂区调速控制问题,提出3种调速控制方案,仿真计算显示,在满足27 t轴重通用货车调速控制、难行车不利溜放、最短溜放距离等约束下,增加布顶与调整坡度相结合的方案最优。     

Surface chlorophyll in the Black Sea over 1978–1986 derived from satellite and in situ data

Absolute values of chlorophyll a concentration and its spatial and seasonal variations in the Black Sea were assessed by using satellite CZCS and in situ data. Since the satellite CZCS had operated for the 1978–1986 period, CZCS data was used for assessing the past state of the Black Sea just before the onset of drastic changes observed in late 1980s. The approach used for the calculation of the absolute values of chlorophyll a concentration from CZCS data was based on the direct comparison of in situ chlorophyll a data and those of CZCS and by applying the algorithm developed for the transformation of CZCS data into chlorophyll a values. CZCS Level 2 data related with pigment concentration having a spatial resolution of 1 km at nadir were used. The daily Level 3 files were derived by binning Level 2 values into 4-km grid cells and the monthly and seasonal Level 3 files were created by averaging the daily Level 3 files over the corresponding period. In situ chlorophyll a data were obtained by spectrophotometric and fluorometric methods in 15 scientific cruises over the 1978–1986 period. Total number of ship-measured data used for the comparison with those CZCS values was 590.Chlorophyll a concentration (Chl) was derived from CZCS values (C) with regression equations Chl=kC; the coefficient of transformation k was calculated from six different data sets by taking into account distinctions between subregions and seasons. The reasons for difference in the k values have been analyzed.Statistical comparison of the chlorophyll a values measured in situ and those derived from CZCS data was based on log-transformed data and gave the following results: regression SLOPE=0.842, regression INTERCEPT=−0.081, coefficient of determination (R²)=0.806, root–mean–square ERROR=0.195. The mean monthly chlorophyll a distributions derived from CZCS data over 1978–1986 have been constructed and the mean seasonal chlorophyll a values in different regions have been calculated and analyzed. The significant difference in chlorophyll concentration between the western shelf regions and the open part of the Black Sea has been demonstrated, especially in warm season. At almost all seasons, the highest chlorophyll concentration is observed in the western interior shelf region which is under strong influence of Danube. The summer mean chlorophyll concentration in this region is 18 times higher than that in the open parts and about nine times higher than in the eastern shelf region. The greatest seasonal variations are observed in the open part of the Black Sea: chlorophyll concentration in cold season is four to six times higher than in summer and three to five times higher than in April and October. To the contrary, in the western interior shelf regions, the concentration is higher in May–October (about twice than that in November–March). Seasonal variations in the western outer shelf regions are smoothed out as compared with both the western interior shelf and the open regions.     

27SiMnMoV钢渗碳工艺性能的研究

本文采用真空渗碳方法,以20CrMnTi 钢为对比对象,研究了27SiMnMoV 钢的渗碳工艺性能.结果表明:27SiMnMoV 钢与20CrMnTi 钢相比,渗碳层中碳化物细小;碳浓度梯度小;硬度分布平缓;力学性能指标α_k、σ_(bb)、f 均要高些.故27SiMnMov 钢的渗碳工艺性能优于20CrMnTi钢.     

27t轴重混编货车作用下既有线钢筋混凝土梁受力特性试验研究

我国研究发展27 t轴重通用货车意义重大,不但可满足铁路货运发展的迫切需要,还能使我国铁路货运全面达到国际重载要求。通过在京广线试验区段内选择跨度3.4,6.7,13.06 m钢筋混凝土梁进行实车试验,获得了27 t轴重货车不同装载等级、不同速度级工况下梁体的钢筋应力、混凝土应力、挠度和挠度动力系数,以及应力和挠度与货车邻轴距的关系等实测数据。初步掌握了27 t轴重货车对小跨度钢筋混凝土梁的作用特征,为27 t轴重货车在既有线的试运行提供了技术支撑。针对试验过程中发现的问题和既有线桥涵实际情况,提出了既有线开行27 t轴重货车时需开展的工作建议。     

高锌铝基合金等温处理过程中组织的演变

对高锌铝基ZA27合金重新加热到固液两相区进行等温处理,研究了等温处理过程中组织的变化,并对有关组织的成分进行了测定.结果表明,等温处理时随时间的延长,共晶组织中的低熔点富Znη相熔化,Zn原子向初生晶内部扩散.保温20 m in时,初生晶粒由铸态时的等轴晶变为近球状晶,晶粒中的η相有熔化现象.     

Na3La9B8O27:Ln3+(Ln=Eu或Tb)荧光体的制备与发光性能

采用高温固相合成技术、溶胶-凝胶法、助熔剂法制备了Na3La9B8O27：Ln^3＋（Ln=Eu或Tb）荧光体,确定了固相反应制备该荧光体适宜的组分re．g,j．分别测试、解释了Na3La9B8O27：Ln^3＋和Na3La9B8O27：Tb^3激发光谱和发射光谱．比较了不同方法制备的荧光体的发光效率,并对产生的差异进行分析和讨论,助熔剂法得到的荧光体发光效率最高．