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为构建更具代表性的机动车行驶工况,实测采集福州地区1辆机动车共20d的真实驾驶数
据,选取14个特征参数表征运动学片段信息,运用主成分分析和K-means聚类划分运动学片段聚类,根据聚类中心的距离筛选备选片段并随机组合构建工况集合。提取11个特征参数计算构建工况的误差,选择集合中误差最小的工况作为构建工况,提出利用混合约束自编码器构建工况优化模型,并研究参数标定方法,最终将平均误差由2.97%缩小到2.39%。混合约束自编码器模型的分析验证结果表明,优化策略符合实际情况,可以有效避免随机选择带来的误差不确定性,验证了所提出行驶工况构建流程的合理性,并提升了工况预测的精确度,得到模型参数推荐值。对实现碳达峰目标下的机动车碳排放预测及排放控制具有重要的现实作用和意义。 相似文献
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为了制备针对EGFRvⅢ的特异性多克隆抗体,在实验中利用耦联了血蓝蛋白的EGFRvⅢ特异性的PEP3合成短肽进行动物免疫,制备抗血清;构建了pET30a/E33XI原核表达载体,表达和纯化了融合蛋白His-E33(EGFRvⅢ的部分胞外区);以此融合蛋白作为抗原制备了抗体纯化柱,通过柱上纯化的方法从抗血清中提取针对EGFRvⅢ的特异性抗体.利用U87、U251、HeLa和HEK-293细胞进行免疫组化实验来检验抗体的特异性. 相似文献
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Wei Zhang Chao Wu Wenshan Yang Shanshan Xu Shaofei Ren . China Ship Development Design Center Wuhan China . College of Shipbuilding Engineering Harbin Engineering University Harbin China 《船舶与海洋工程学报》2011,(3)
Due to the unique structural mode and material property of a composite sandwich plate, related research such as fragment impact resistance of a composite mast is short of publication and urgent in this field. In this paper, the commonly accepted sandwich core board theory was modified. Damage caused by a fragment attack was simulated onto a sandwich plate model built with solid and shell elements. It was shown that shear failure and vast matrix cracking are the main reasons for outer coat damage, and tensio... 相似文献
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Objective To obtain the gene of murine Single chain Fv fragment (ScFv) against haman cervical cancer and to express it in E. coli. Methods The variable region gene fragments of the heavy and light chains, which were amplified respectively using recombinant DNA techniques from CsA125 hybridama cells, were spliced together through a flexible linker to ScFv against human cervical cancer. The ScFv genes were then cloned into expression vector pCANTAB 5E and expressed in E. coli HB2151 and TG1 respectively. The soluble ScFv were characterized by SDSPAGE and Western blot. The antigen-binding activities of the soluble and phage displayed ScFv were assayed by ELISA and cell immunohistochemical analysis. Results The expressed ScFv antibodies were soluble and phage displayed. soluble ScFv secreted and expressed in E. coli HB2151 induced by IPTG were confirmed with SDS-PAGE, Western blot and ELISA. The specific binding capacity of the soluble and phage displayed ScFv to the surface associated antigen of human cervical cancer cell line was further confirmed with immunohistochemical studies. Conclusion The soluble and phage displayed ScFv expressed in E. coll against haman cervical cancer showed high, specific affinity for the cervical cancer cell line surface associated antigen. 相似文献
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简要回顾了陶瓷/金属复合靶板的研究现状.根据船用钢的吸能特点和船用陶瓷轻型复合装甲的特殊性,将10 g破片模拟弹侵彻陶瓷/船用钢的过程分为两个阶段,分别计算了破片模拟弹的侵蚀变形能和船用钢背板的隆起-碟型变形吸能,并将后者分为径向弯曲变形能、径向膜托伸变形能及周向弯曲变形能等,给出了各种能量的计算公式,建立了计算弹道极... 相似文献
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