微生物分子分类方法的简述

微生物的分类经历了传统分类方法、数值分类方法、化学分类方法和分子分类方法，而分子生物学的迅速发展使我们对微生物分类有了更深的认识，目前在微生物分类中常用的分子分类方法主要是碱基组成(G Cmol％)的分析、碱基序列的分析、基于PCR技术的分析、基于电泳技术的分析。     

Cloning and sequencing of a DNA fragment encoding N37 apoptotic peptide derived from p53

Objective It was reported that p53 apoptotic peptide (N37) could inhibit p73 gene through being bound with iASPP, which could induce tumor cell apoptosis. To further explore the function of N37, we constructed the cloning plasmid of DNA fragment encoding p53 (N37) apoptotic peptide by using DNA synthesis and molecular biology methods. Methods According to human p53 sequence from the GenBank database, the primer of p53(N37) gene was designed using Primer V7.0 software. The DNA fragment encoding p53 (N37) apoptotic peptide was amplified by using self-complementation polymerase chain reaction (PCR) method and cloned into the pGEM-T Easy vector. The constructed plasmid was confirmed by endonuclease analysis and sequencing. Results The insertion of objective DNA fragment was confirmed by plasmid DNA enzyme spectrum analysis, p53 (N37) gene was successfully synthesized chemically in vitro. The sequencing result of positive clone was completely identical to the human p53(N37) sequence in GenBank using BLAST software (http://www. ncbi. him. nih. gov/cgi-bin /BLASTn). Conclusion The cloning of DNA fragment encoding p53(N37) apoptotic peptide was constructed by using DNA synthesis and pGEM-T Easy cloning methods. With the constructed plasmid, we could further investigate the function of N37 peptide.     

正电子发射断层摄影

分子显影技术导致了细胞生物学、分子生物学和分子遗传学等的原理在传统医学显影技术中的应用。该方法越来越成为包括核医学、核磁共振显影和超声显影等众多显影方法的中心。     

脆性组氨酸三联体基因与肿瘤关系的研究进展

脆性组氨酸三联体(fragile histine traid,FHIT)基因是近年来发现的一个新的候选抑癌基因,是将染色体脆性位点和肿瘤相联系的第一个分子生物学证据。因与传统抑癌基因在肿瘤组织中的表达形式不同,故目前大多数学者认为应暂将其归类于候选抑癌基因。作为一种新的候选抑癌基因,FH     

图着色问题的表面DNA算法

色数是图论中一个重要的参数，近年来受到人们的广泛关注。图着色问题是名的NP－完全问题。章给出了一个用分子生物学技术解决图着色问题的算法。该算法具有高度的并行性和可靠性。     

大骨节病软骨细胞发生坏死的分子生物学机制

目的综述大骨节病软骨细胞发生坏死的分子生物学研究进展。方法采用文献调查方法,对比分析国内外有关大骨节病与关节病的研究现状。结果大骨节病核心家庭有家庭聚集性趋势,患者软骨除表现有深层软骨细胞坏死外,还表现有软骨细胞过度凋亡和去分化;与骨关节病比较,大骨节病尚未开展STR多态性、软骨胶原基因和基因芯片方面的研究。结论应采用STR多态性、软骨胶原基因、基因芯片技术深入研究大骨节病分子生物学机制。     

试论寒武纪生命大爆发对达尔文进化学说的质疑

寒武纪生命大爆发,对达尔文渐进连续的进化论发出了诘难和挑战,本文根据20世纪末现代分子生物学研究的最新成果,试探性地解释了寒武纪生命大爆发的成因和机制。     

PRELIMINARY STUDY OF A NOVEL HUMAN PAPILLOMAVIRUS TYPE 16L1／E6-E7 CHIMERIC RECOMBINANT DNA VACCINE

Objective Preparations of HPV16 L1/E6 and L1/E7 prophylactic and therapeutic DNA vaccines. Methods The nucleotides within HPV16 E6 and E7 genes, which are responsible for viral transforming activity, were mutated by mage primer site-directed mutagenesis method. The correctly mutated E6 and E7 fragments were separately cloned into an eukaryotic expression vector pVAX1, together with HPV16 L1 gene, generating chimeric recombinants plasmids 1MpVAX1-L1E6, 2MpVAX1-L1E6, 1MpVAX1-L1E7, 2MpVAX1-L1E7 and 3MpVAX1-L1E7. CHO cells were transiently transfected with the individual DNA vaccines by calcium phosphate method. Target protein expressions in the extracts of the transfected cell lines were measured by ELISA and immunohistochemistry, with HPV16 L1 and E6 specific monoclonal antibodies. Results ELISA assays showed the P/N ratios in the cell extracts transfected with L1E6 and L1E7 plasmids were more than 2.1. Immunohistochemistry revealed brownish precipitant signal in cytoplasm and nuclei of the transfected cells. Conclusion Successful constructions of prophylactic and therapeutic DNA vaccine plasmids lay solid foundation for future animal experiment and clinical trial.