孕妇外周血PCR技术用于胎儿产前性别诊断的可靠性分析

采用多聚酶链反应（ＰＣＲ）技术，以Ｙ染色体特异重复序列Ｙ１．１、Ｙ１．２和ＳＲＹ基因编码区Ｐ１、Ｐ２为引物，分析了用孕妇外周血进行胎儿性别诊断的可靠性，并与绒毛样本进行了对比。结果表明：用孕妇外周血时，Ｙ１．１，Ｙ１．２引物的诊断符合率为７５％；Ｐ１、Ｐ２引物不能扩增出Ｙ特异ＤＮＡ序列。而用绒毛样本时，两种引物的诊断符合率均为１００％。结果提示：孕妇外周血ＰＣＲ技术用于胎儿性别诊断尚有待改进，而采用绒毛样本进行胎儿性别诊断更为可靠。     

莫斯科地铁的特点及对我国地铁的启示

全面介绍莫斯科地铁在线网规模、车站设计、运营组织等方面的理念及特点,详细分析莫斯科地铁客流量大、运营效率高的原因,以期为我国未来地铁发展提供借鉴.     

公路建设项目征地拆迁补偿费用确定与控制方法研究

文中以广东省公路建设项目征地拆迁费用的确定为例,以现行实施的征地拆迁补偿相关规定为基础,探讨征地数量和标准的计量、计算方法,建立征地拆迁标准动态数据库,以期对征地拆迁费用的确定和控制提供思路和实际操作指导。     

Optimal bus fleet management strategy for emissions reduction

Transportation is a major cause for environmental degradation via exhaust emissions. For many transit-oriented metropolitan areas, bus trips often constitute a sizeable mode share. Managing the bus fleet, in particular updating buses to comply with the newer emissions standards, therefore, can have a substantial impact on transportation-induced air quality. This paper presents the approach of remaining life additional benefit–cost (RLABC) analysis for maximising the total net benefit by either early-retiring or retrofitting the current bus fleet within their lifespans. By referring to the net benefits for different bus types estimated by RLABC analysis, the most beneficial management scheme for the current bus fleet can be identified. Optimal bus fleet management (BFM) models based on the RLABC analysis for the operator and the government are developed. Then a government subsidy plan is produced to achieve win–win solutions, which will offer efficient and flexible management schemes. To illustrate the approach, the largest bus company in Hong Kong, which carries more than 23% of the total trips in Hong Kong, is taken as a case study example. Instead of adopting a fixed retirement plan, such as replacing buses at the age of 17 as is currently practised, the proposed method develops an optimal BFM scheme that progressively phases out buses or retrofits them. This study produces promising results to demonstrate the large benefit of this approach for optimal bus fleet management.     

A cost-competitiveness analysis of charging infrastructure for electric bus operations

This study investigates the cost competitiveness of different types of charging infrastructure, including charging stations, charging lanes (via charging-while-driving technologies) and battery swapping stations, in support of an electric public transit system. To this end, we first establish mathematical models to investigate the optimal deployment of various charging facilities along the transit line and determine the optimal size of the electric bus fleet, as well as their batteries, to minimize total infrastructure and fleet costs while guaranteeing service frequency and satisfying the charging needs of the transit system. We then conduct an empirical analysis utilizing available real-world data. The results suggest that: (1) the service frequency, circulation length, and operating speed of a transit system may have a great impact on the cost competitiveness of different charging infrastructure; (2) charging lanes enabled by currently available inductive wireless charging technology are cost competitive for most of the existing bus rapid transit corridors; (3) swapping stations can yield a lower total cost than charging lanes and charging stations for transit systems with high operating speed and low service frequency; (4) charging stations are cost competitive only for transit systems with very low service frequency and short circulation; and (5) the key to making charging lanes more competitive for transit systems with low service frequency and high operating speed is to reduce their unit-length construction cost or enhance their charging power.     

Robust weekly aircraft maintenance routing problem and the extension to the tail assignment problem

In this paper, we study two closely related airline planning problems: the robust weekly aircraft maintenance routing problem (RWAMRP) and the tail assignment problem (TAP). In real life operations, the RWAMRP solution is used in tactical planning whereas the TAP solution is implemented in operational planning. The main objective of these two problems is to minimize the total expected propagated delay (EPD) of the aircraft routes. To formulate the RWAMRP, we propose a novel weekly line-of-flights (LOF) network model that can handle complex and nonlinear cost functions of EPD. Because the number of LOFs grows exponentially with the number of flights to be scheduled, we propose a two-stage column generation approach to efficiently solve large-scale real-life RWAMRPs. Because the EPD of an LOF is highly nonlinear and can be very time-consuming to accurately compute, we propose three lower bounds on the EPD to solve the pricing subproblem of the column generation. Our approach is tested on eight real-life test instances. The computational results show that the proposed approach provides very tight LP relaxation (within 0.6% of optimal solutions) and solves the test case with more than 6000 flights per week in less than three hours. We also investigate the solutions obtained by our approach over 500 simulated realizations. The simulation results demonstrate that, in all eight test instances, our solutions result in less EPDs than those obtained from traditional methods. We then extend our model and solution approach to solve realistically simulated TAP instances.     

基于GPS载波相位信号确定运动体姿态的关键技术

应用GPS载波相位信号确定运动体姿态,针对二维两天线系统情况,设计了姿态测量系统的硬件结构及算法流程框图,文章介绍了GPS姿态测量的关键技术：GPS姿态观测方程、整周模糊度的解算、误差的消除。从系统设计角度证明了技术的可行性。     

集聚经济视角下轨道交通投资对城市系统均衡的影响

随着城镇化进程加快,城市规模增加所带来的集聚效应和交通拥堵等负外部性之间的矛盾越来越突出,优化城市交通基础设施建设是缓解该矛盾的重要举措。本文同时考虑了集聚经济和交通拥挤两种外部性,构建并求解了一个两区域城市空间结构模型,分三种情况探究了轨道交通的投资引入和道路容量的优化对城市规模和城市空间结构均衡的影响。结果表明,轨道交通的投资引入能够增加城市人口规模和社会福利,使城市结构更加紧凑。但是,随着集聚经济水平的提高,城市规划者应逐步完善公交配套设施,以降低公交拥挤成本,提高公交出行分担率;还应制定相应的福利政策,避免由于个体效用下降而导致人口流出。     

榆林地区风积沙的工程分类

为了研究榆林地区风积沙的工程技术分类,依托陕西省榆林市境内穿越风积沙地区的国道210陕蒙段和省道204榆靖段的改扩建工程,采集沿线风积沙样,对沙样进行了筛分、含泥量、液塑限等试验,分析了筛分试验结果和级配指标,确定了风积沙分类的划分依据,对榆林地区风积沙进行了工程分类,并通过击实和强度试验,进行了验证性分析.研究结果表...