| APPLICATION OF DIGOXIGENIN LABELED PROBES TO DETECTION AND TYPING OF HERPES SIMPLEX VIRUS |
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| 引用本文： | 楚雍烈,刘巧芳,刘正风,杜忆华,刘延娜.APPLICATION OF DIGOXIGENIN LABELED PROBES TO DETECTION AND TYPING OF HERPES SIMPLEX VIRUS[J].西安交通大学学报(英文版),1994(2). |
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| 作者姓名： | 楚雍烈 刘巧芳 刘正风 杜忆华 刘延娜 |
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| 作者单位： | Chu Yonglie; Liu Qiaofang; Liu Zhengfeng;Du Yihua; Liu Yanna (Department of Microbiolosry and Immunology，Xi'an Medical University，Xi'an 710061) ( Research Laboratory of Nuclear Medicine) |
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| 摘 要： | ＣｈｕＹｏｎｇｌｉｅ（楚雍烈）；ＬｉｕＱｉａｏｆａｎｇ（刘巧芳）；ＬｉｕＺｈｅｎｇｆｅｎｇ（刘正风）；ＤｕＹｉｈｕａ（杜忆华）；ＬｉｕＹａｎｎａ（刘延娜）ＡＰＰＬＩＣＡＴＩＯＮＯＦＤＩＧＯＸＩＧＥＮＩＮＬＡＢＥＬＥＤＰＲＯＢＥＳＴＯＤＥＴＥＣＴＩＯＮ...
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APPLICATION OF DIGOXIGENIN LABELED PROBES TO DETECTION AND TYPING OF HERPES SIMPLEX VIRUS |
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| Chu Yonglie, Liu Qiaofang, Liu Zhengfeng,Du Yihua, Liu Yanna.APPLICATION OF DIGOXIGENIN LABELED PROBES TO DETECTION AND TYPING OF HERPES SIMPLEX VIRUS[J].Academic Journal of Xi’an Jiaotong University,1994(2). |
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| Authors: | Chu Yonglie Liu Qiaofang Liu Zhengfeng Du Yihua Liu Yanna |
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| Abstract: | A total of 44 HSV isolates from the patients was detected and typed by using HSV typespecific DNA probes labeled with Digoxigenin and 32 P respectively. 25 isolates were identified to be HSV-1, 19 were HSV-2. Comparison of the typing results showed that the sensitivity and specificity of the Digoxigenin-labeled probe were equal to those of the 32 P-labeled Probe, and the typing rate of HSV isolates was 100%. The Dig-labeled probes are nonradioactive and the labeling technigue is simple, the Dig-labeled probes can be stored and reused, so they are more valuable for clinical application. |
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| Keywords: | herpes simplex virus typing Digoxigenin labeling nucleoacid hybridization |
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