脊髓损伤后大鼠后肢运动功能恢复不同评分标准的比较

目的比较脊髓损伤(SCI)后大鼠后肢运动功能恢复的不同评分标准的优劣。方法40只SD成熟雌性大鼠随机分为正常组(Normal组)、急性脊髓中度损伤组(SCI组)及对照组(CON组),其中SCI组采用改良的Allen打击法,CON组仅行T10椎板切除术。术后1、2、3、4、6周观察大鼠后肢神经功能恢复的情况并记录结果。评价标准分别为:斜板试验评分、改良Tarlov评分及BBB评分。结果SCI组与CON组比较,斜板试验临界角度在1-6周时,均有所减小(P<0.05),尤以第1周时减少更甚(P<0.01);改良Tarlov评分第1、2、3、4周时,分值间的差别非常明显(P<0.01),第6周时,未见变化(P>0.05);而BBB评分各时间点的区分程度非常明显(P<0.01)。结论BBB评分对SCI模型运动功能评价具有明显优势,可作为今后研究的标准评分法。

A compare of hindlimbs locomotor function recovery after spinal cord injury in rats

Objective To contrast the different scale rule of hindlimbs locomotor function recovery after the rat spinal cord was injured.Methods A total of 40 rats were divided into 3 groups: normal group,spinal cord injury group(SCI)(Modified Allen method),and control group(CON)(only T_(10) laminectomy).To observe nerve functional recovery of hindlimbs after operation in 1w,2w,3w,4w,6w respectively.Score standard: Inclined plane method,Modified Tarlov grading,BBB scale and recording results.Results To contrast SCI group and control group,critical angle in incline plane test was decreased in 6 weeks(P<0.05),especially in 1 week(P<0.01);Modified Tarlov grading was compared: 1w,2w,3w,4w, the difference of scale was greatly different(P<0.01),but in 6w this change disappeared(P>0.05);in convalescence stage(from 1w to 6w),the degree of every one of BBB scales was distinguished(P<0.01).Conclusion It is reasonable that the BBB scale is a standard test for SCI locomotor function assessment in rats.