蒙脱石对大鼠肠、血管循环灌流液中肌酐的吸附作用

目的研究蒙脱石对血管灌流液中的肌酐向肠道扩散的促进作用和阻止肠灌流液中肌酐的吸收作用。方法大鼠肠道、血管分别用含80mg/L肌酐的血管灌流液和肠灌流液循环灌流,碱性苦味酸法测定肌酐水平。结果当血管用含肌酐的血管灌流液循环灌流、肠道用不含肌酐的肠道灌流液循环灌流时,血管灌流液中的肌酐水平逐渐下降,而肠道灌流液中的肌酐水平逐渐升高;同时在肠道中加入蒙脱石时,蒙脱石0.5g/kg和1.0g/kg组的血管灌流液和肠道灌流液中的肌酐水平均明显低于对照组(P<0.01)。当肠道用含肌酐的肠道灌流液循环灌流、血管用不含肌酐的血管灌流液循环灌流时,肠道灌流液中的肌酐水平逐渐下降,而血管灌流液中的肌酐水平逐渐升高;同时肠道中加入蒙脱石后,蒙脱石0.5g/kg和1.0g/kg组肠道灌流液和血管灌流液中肌酐水平明显低于对照组(P<0.01)。结论肌酐可从血管向肠道扩散,并可在肠道再吸收;蒙脱石能加速肌酐向肠道的扩散,减少肌酐在肠道的吸收。

Montmorillonite absorbs creatinine in circulating intestinal and vascular perfusates in rats

Objective To study the effect of montmorillonite in promoting diffussion of creatinine from blood vessel to intestine and preventing absorption of creatinine in intestinal perfusate.Methods The intestine tract and blood vessel of rats were perfused circularly with intestinal tract perfusate or blood vessel perfusate containing creatinine 80 mgL,respectively.The concentration of creatinine was determined by basic picric acid method.Results When the blood vessel and intestinal tract were perfused circularly by vascular perfusate containing creatinine and intestine perfusate without creatinine,respectively,the concentration of creatinine in vascular perfusate decreased little by little,and the concentration of creatinine in intestine perfusate increased slowly.The concentration of creatinine of intestine and blood vessel perfusates was decreased in montmorillonite of 1.0 gkg and 0.5 gkg groups compared with that in control group (P<0.01).The blood vessel and intestinal tract were perfused circularly by vascular perfusate without creatinine and intestine perfusate with creatinine,respectively,the concentration of creatinine in intestine perfusate decreased little by little,and the concentration of creatinine in vascular perfusate increased slowly.When montmorillonite was administered into the intestinal tract,the concentration of creatinine in intestine and blood vessel perfusates of montmorillonite of 1.0 gkg and 0.5 gkg group was decreased obviously (P<0.01).Conclusion Creatinine can diffuse from blood vessel to intestine and is reabsorbed in intestine.Montmorillonite can promote the diffussion of creatinine to intestine tract and can decrease absorption of creatinine in intestinal tract.