假型逆转录病毒载体高效介导兔平滑肌细胞基因转染

目的 构建假型逆转录病毒载体MuLV/VSV-G,并用于转染兔平滑肌细胞,为兔平滑肌细胞基因转染寻找一种高效的载体.方法 构建含有报道基因lacZ的假型逆转录病毒载体MuLV/VSV-G,测定滴度,并转染兔平滑肌细胞,观察其转导效率.并与MuLV的转导效率进行比较.结果 构建的MuLV/VSV-G载体,病毒滴度为6～7.8×10~6CFU,转染兔平滑肌细胞的效率是(92±12)%.而MuLV的转导效率为(24±5)%.结论 成功构建了假型逆转录病毒载体MuLV/VSV-G载体,该载体作为一种高效的载体可用于兔平滑肌细胞基因转染.

High-efficiency gene transfer into rabbit smooth muscle cells by pseudotyped retroviral vector

Objective To construct pseudotyped retroviral vector MuLV/VSV-G and transfer it into rabbit smooth muscle cells (SMC) in order to provide a high-efficiency vector for SMC gene transfer. Methods We constructed pseudotyped retroviral vector MuLV/VSV-G containing the previously reported gene lacZ, determined the titer, and determined the efficiency of gene transfer into SMC mediated by pseudotyped retroviral vector MuLV/VSV-G. Finally the transfer efficiency was compared with that by MuLV. Results MuLV/VSV-G vector was constructed. The titer of the vector was 6-7.8×10~6CFU, the transfer efficiency was (92±12)% by using MuLV/VSV-G vector and (24±5)% by MuLV vector. Conclusion Pseudotyped retroviral vector MuLV/VSV-G which was constructed successfully is a kind of high-efficiency gene transfer vector in smooth muscle cells.