| 致癌物染毒大鼠体内DNA加合物的检测 |
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| 引用本文： | 易建华,陈玉华,王莉. 致癌物染毒大鼠体内DNA加合物的检测[J]. 西安交通大学学报(医学版), 1993, 0(1) |
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| 作者姓名： | 易建华 陈玉华 王莉 |
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| 作者单位： | 西安医科大学卫生系职业卫生学教研室，西安医科大学卫生系职业卫生学教研室，西安医科大学卫生系职业卫生学教研室 西安 710061，西安 710061，西安 710061 |
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| 摘 要： | 本文报道了我们用联苯胺以及联苯胺衍生偶氮染料刚果红,依文思蓝经口染毒大鼠后用~(32)P后标记方法对其肝脏中的DNA加合物的检测结果,3种物质均检出1种DNA加合物,其含量分别为18.17,1.03,1.74μmol/kgDNA。结果表明联苯胺衍生偶氮染料经偶氮还原产生的联苯胺或联苯胺衍生物,能同联苯胺样在大鼠肝内形成加合物,这种DNA加合物很可能是接触联苯胺衍生偶氮染料的人群膀胱癌发病高的原因。
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| 关 键 词： | 致癌物 联笨胺 DNA加合物 ~(32)P后标记测定 |
~(32)P-POSTLABELING TEST OF DNA ADDUCTS FORMED IN THE LIVERS OF RATS TREATED WITH CARCINOGEN |
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| Yi Jianhua,Chen Yuhua,Wang Li. ~(32)P-POSTLABELING TEST OF DNA ADDUCTS FORMED IN THE LIVERS OF RATS TREATED WITH CARCINOGEN[J]. Journal of Xi‘an Jiaotong University:Medical Sciences, 1993, 0(1) |
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| Authors: | Yi Jianhua Chen Yuhua Wang Li |
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| Affiliation: | Yi Jianhua;Chen Yuhua;Wang Li Department of occuptional hygiene |
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| Abstract: | DNA in the livers of rats was isolated 24 h af-ter oral administration of benzidine, Congo red andEvan's blue, DNA adducts in the livers of ratswere investigated by a ~(32)P-postlabeling assay. Thelevel of DNA adducts in the livers of rats after oraladministration of benzidine, congo red andEvan'blue were 18. 17, 1. 03 and 1. 74μmol/kgDNA respectively. The results showed that themetabolites of the benzidine derivative azo dyes canform adducts with DNA in the livers of rats.These DNA adducts probably are one of the rea-sons that the incidence of the bladder cancer rosein the people group who are only exposed to benzi-dine derivative azo dyes. |
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| Keywords: | carcinogenicity benzidine DNA adducts ~(32)P-postlabeling assay |
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