乙型肝炎病毒基因型检测及其与肝功能的关系

目的 探讨乙型肝炎病毒基因型与肝功能的关系.方法 采用微板核酸分子杂交ELISA法检测93例不同临床类型的乙型肝炎患者的基因型并同时测定肝功能.结果 93例不同临床类型乙型病毒性肝炎患者中,B型24例(25.81%),C型59例(63.44%),D型5例(5.38%),混合型5例(B/D 3例,C/D 2例,占5.38%).93例不同临床类型的乙型肝炎患者中,以C基因型为主,其次为B基因型,部分以D型和混合型存在,无A、E、F型.按照慢性乙型肝炎、亚急性重型肝炎、肝硬化的顺序,C基因型的检出率逐渐增多,B基因型的检出率逐渐减少.肝癌患者C基因型的检出率没有依次增高.C基因型各型乙型肝炎患者的ALT、AST、TBIL均高于B型,ALB低于B型,但差别无统计学意义.结论 西安地区病毒性肝炎以C基因型为主,部分以B、D及混合型存在,未发现A、E、F型.除肝癌外,C型的检出率随临床类型的加重而增多.

Detection of hepatitis B virus genotype and the relationship between hepatitis B virus genotype and the function of liver

Objective To investigate the relationship between hepatitis B virus (HBV) genotype and liver function. Methods The method of microboard nucleate molecular hybridization was employed to detect the genotype in 93 HBV patients of different clinical types and the function of liver. Results Among the 93 HBV patients of different clinical types, there were 24 cases of genotype B (25.81%), 59 cases of genotype C (63.44%), 5 cases of genotype D (5.38%), and 5 cases of mixed type (3 cases of B/D, 2 cases of C/D, 5.38%). Therefore, genotype C took up the largest proportion, followed by genotype B, and then D and mixed genotypes, but there was no genotype A, E or F. The detection rate of genotype C increased according to the sequence of chronic hepatitis B, subacute severe hepatitis and hepatocirrhosis while the detection rate of genotype B decreased gradually. However, the detection rate of genotype C in hepatocellar carcinoma did not rise correspondingly. The levels of ALT, AST and TBIL of genotype C were higher than those of genotype B, but the level of ALB in genotype C was lower than that of genotype B. None of the differences had significance. Conclusion Most of HBV genotypes in Xi'an were C, some of them were B, D and mixed genotypes, but no genotype A, E or F was detected. Except hepatocellar carcinoma, the detection rate of genotype C rose according to the severity of clinical type.