负压对供皮区创面再上皮化速度的影响

目的观察负压对猪供皮区创面再上皮化速度的影响。方法5头小猪,每头猪的背部各形成面积为5 cm×10 cm断层皮片供皮创面。实验组采用连续120 mmHg(1 mmHg=0.133 kPa)负压治疗,对照组用凡士林纱布换药。结果负压治疗组创面再上皮化速度显著提高,治疗后4-6 d创面即愈合,较对照组提前2-4 d;创面炎症反应明显减轻;毛囊上皮、再生的基底以及腺上皮细胞增殖活跃,S期及G2 M期细胞比例增多(P<0.01);细胞核增殖抗原表达显著增强。结论负压可以显著提高供皮区创面再上皮化速度。

Effect of negative pressure on the rate of skin donor site re-epithelialization in pig model

Objective To test the effect of negative pressure on the rate of skin donor site re-epithelialization in pig model.Methods Five pigs were selected for this study,and two split-thickness skin graft defects were harvested from the back of each animal.Half of the donor sites were treated with 120 mmHg negative pressure as the experimental group for 10 days and half were treated with vaseline gauze as the control.Changes in each wound were assessed every two days.Results The rate of re-epithelialization increased significantly.The donor sites healed after 4 to 6 days of negative pressure treatment, 2 to 4 days earlier than the control group.The level of inflammatory reaction occurring in wounds treated with negative pressure appeared less severe than that in wounds treated with vaseline gauze.Negative pressure could facilitate cell proliferation.The ratios of S,G2 and M phase increased significantly,and higher expression of PCNA was observed and detected in hair hollicle epithelium,regenerative basal cells and glandular epithelium on day 4 and 8 after negative pressure treatment.Conclusion Negative pressure could significantly increase the rate of skin donor site re-epithelialization