CpG寡核苷酸对人外周血单个核细胞的免疫刺激作用

目的　观察CpGODN、GM CSF、CD40 L对正常人外周血单个核细胞 (PBMC)的免疫刺激作用 ,以期筛选出一种高效的疫苗佐剂。方法　用CpGODN 2 0 0 6、GM CSF、CD40 L刺激正常人PBMC ,48h后采用ELISA法检测培养上清液中IFN γ、IL 12及IL 10水平 ;应用免疫荧光法检测PBMC上CD40、CD86、HLA DR的表达。结果　CpGODN 2 0 0 6能诱导正常人PBMC分泌较高水平的IL 12、IFN γ ,抑制IL 10的分泌 ;上调PBMCCD40、CD86、HLA DR表达的作用最强。结论　CpGODN 2 0 0 6是一种免疫刺激作用强大的新型佐剂 ,可用于多种疫苗的制备。

The immunostimulatory effects of CpG oligonucleotide on human peripheral blood mononuclear cells

Objective To select a more effective vaccine adjuvant by observating the immnunostimulatory effects of CpG ODN 2006, GM-CSF and CD40-ligand on normal human peripheral blood mononuclear cell (PBMC). Methods After PBMCs were incubated with CpG ODN 2006, GM-CSF, and CD40-L for 48 hours, the levels of IFN-γ, IL-12 and IL-10 in culture supernatant were detected by ELISA and the expressions of the CD40, CD86, HLA-DR on PBMC were examined by immunofluorescence assay.Results Among the three adjuvants, CpG ODN 2006 had the most remarkable stimulatory effects on inducing the production of IL-12 and IFN-γ, and on depressing IL-10 production and on up-regulating expression of CD40, CD86, HLA-DR of PBMC from normal human.Conclusion CpG ODN 2006 can effectively activate the immune system of the human body and may be used as a vaccine adjuvant.