硒对大鼠胰腺蛋白质合成和胰淀粉酶、糜蛋白酶活性的影响

本文以低硒的克山病病区粮为基础饲料,研究了改变饲料中硒含量单一因素对[~3H]—亮氨酸参入胰蛋白质的速率和胰淀粉酶、糜蛋白酶活性的影响。结果发现硒缺乏可使大鼠胰腺组织蛋白质合成速度和胰淀粉酶、糜蛋白酶活性下降。结合低硒组大鼠胰腺GSH-Px活性下降,TBA值升高,推测由于缺硒大鼠胰腺中脂质过氧化物和其代谢分解产物丙二醛积蓄,导致生物膜的结构破坏、DNA和RNA的结构和含量改变、能量供给不足,以及对蛋白质、酶分子、氨基酸等的破坏而影响蛋白质合成体系的功能,使蛋白质合成速度下降。由于分泌蛋白质的合成下降,则出现胰腺外分泌功能障碍,胰淀粉酶、糜蛋白酶等活力降低。食物补硒后可以保护正常胰腺的结构和功能。

EFFECTS OF SELENIUM IN DIET ON RAT PANCREAS PROTEIN SYNTHESIS AND ENZYMES ACTIVITY

The study was to investigate comparatively the changes of protein synthesis and amylase, chymotrypsin, lipase activity in pancreas from two groups rats fed with selenium deficient diet from a Keshan disease (KD) endemic area (Se: 0.021ppm) and KD diet supplemented with sodium selenite(Se: 0.264ppm). The rats fed with Xian's cereals were taken as the control group. The results of our observations suggest that the dietary selenium of 0.241ppm level is sufficient to stimulate inocroporation of [~3H]-Leucine in protein by rat pancreas slices, to increase activity of amylase, chymotrypsin and lipase, toi ncrease activity of GSH-Px and to decrease TBA value in rat pancreas. In addition, it increases body weight and decreases mortality in rats too. It seems that selenium, by the ways of stimulating protein synthesis and increasing activity of pancreasec enzymes, can protect the pancreas and lead to gain weight in rats.