Toll样受体4在佐剂性关节炎模型大鼠外周血单核细胞中的表达及意义

目的研究佛氏完全佐剂性关节炎(AIA)大鼠外周血单核细胞(PBMC)对Toll样受体4(Toll-like receptor 4,TLR4)、TLR4 mRNA的表达及其与关节炎评分的关系。方法流式细胞仪测定CD14 TLR4 细胞占CD14 细胞的比例和TLR4-PE的平均荧光强度(MIF);Northern blot法测定PBMC TLR4 mRNA的表达量。比较造模后15 d与正常对照组和造模后25 d时CD14 TLR4 /CD14 、MIF及其TLR4 mRNA表达量的差异,并用Mann-WhiteyU检验分析关节炎评分与TLR4 MIF的相关性。结果在造模后15 d,CD14 TLR4 /CD14 、TLR4 MIF和PB-MC TLR4 mRNA的表达较正常对照组和造模后25 d明显增高(P<0.01),而且TLR4 MIF与关节炎的评分有明显的相关性(15 d:r=0.826,P<0.01;25 d:r=0.688,P<0.05)。但造模后25 d,CD14 TLR4 /CD14 、TLR4 MIF和PBMC TLR4 mRNA的表达与正常对照组和造模前相比无统计学意义(P>0.05)。结论TLR4与AIA发病有密切关系,提示固有免疫反应在类风湿性关节炎(RA)及其他自身免疫性疾病的发病中有重要的作用。

Expression and significance of toll-like receptor 4 by peripheral blood mononuclear cell in rats with adjuvant-induced arthritis

Objective To demonstrate the role of innate immunity reaction in rheumatoid arthritis and other autoimmune diseases by studying the expression of toll-like receptor 4(TLR4) by peripheral blood mononuclear cell(PBMC) in rats with adjuvant-induced arthritis(AIA) and analyzing the relationship between expression of TLR4 by PBMC and score of arthritis severity.Methods Both the proportion of CD14 TLR4 cells in CD14 cells and the mean intensity of fluorescence(MIF) of TLR4 cells were analyzed by flow cytometry.Expression of TLR4 mRNA by PBMC was determined by Northern blot.CD14 TLR4 /CD14 ratio,TLR4 MIF and expression of TLR4 mRNA by PBMC in the model group at 15 days after AIA were compared,respectively,with normal control group and model group at 25 days.The correlation between TLR4 MIF and score of arthritis severity was analyzed by Mann-Whitey U test.Results CD14 TLR4 /CD14 ratio,TLR4 MIF and TLR4 mRNA expression in model group at 15 days after AIA were obviously increased compared with those in control and model groups at 25 days(P<0.01),and a good correlation between TLR4 MIF and score of arthritis severity was observed(15 d: r=0.826,P<0.01;25 d: r=0.688,P<0.05),but no significant difference in model group at 25 days after AIA(P>0.05).It indicated that TLR4 participated in the pathogenesis of rheumatoid arthritis.Conclusion TLR4 expressed by PBMC is closely associated with pathogenesis and pathophysiology of rheumatoid arthritis,which suggests that innate immunity response plays an important role in pathogenesis of RA and other autoimmune diseases.